Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1
In order to preliminarily explore the structure, immune and anti-aging activity of capsular polysaccharide (CPS) produced by Lactobacillus plantarum W1. In this study, CPS was extracted and prepared by enzymatic hydrolysis. The absolute molecular weight, functional groups and monosaccharide composit...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2025-02-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030207 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823865252536647680 |
|---|---|
| author | Yongjiao GAO Kun WANG Jing ZHAO Feng ZUO |
| author_facet | Yongjiao GAO Kun WANG Jing ZHAO Feng ZUO |
| author_sort | Yongjiao GAO |
| collection | DOAJ |
| description | In order to preliminarily explore the structure, immune and anti-aging activity of capsular polysaccharide (CPS) produced by Lactobacillus plantarum W1. In this study, CPS was extracted and prepared by enzymatic hydrolysis. The absolute molecular weight, functional groups and monosaccharide composition of CPS were analyzed by gel permeation chromatography system, Fourier transform infrared spectroscopy and high performance liquid chromatography. The immune and anti-aging activities of CPS were investigated using RAW264.7 cells and Caenorhabditis elegans (C. elegans) models. The results showed that CPS was a heteropolysaccharide with an average molecular weight of 5.49×104 Da, consisting of glucose and galactose in a molar ratio of 3.12:1. Furthermore, CPS had been found to enhance the proliferation and phagocytosis of RAW264.7 cells, as well as promote the secretion of NO and cytokines, thereby exerting an immunomodulatory effect. At a concentration of 125 μg/mL, the secretion levels of TNF-α, IL-6 and IL-1β in RAW264.7 cells reached their maximum values, which were 1.26 times, 1.11 times and 1.96 times higher than those in the control group, respectively. Concurrently, CPS significantly enhanced the antioxidant system of C. elegans (P<0.05), prolonged their lifespan, and improved their stress tolerance. The average lifespan of nematodes under normal, oxidative stress and heat shock conditions reached its maximum at 125 μg/mL, with lifespan extension rates of 21.05%±0.21%, 42.86%±0.27% and 50.00%±0.27%, respectively. These findings indicate that CPS exhibits significant biological activity and has the potential to be utilized as functional excipients in food products. |
| format | Article |
| id | doaj-art-7acc5231e0d140c29561970b1c5e4c62 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-7acc5231e0d140c29561970b1c5e4c622025-02-08T08:06:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-01464909910.13386/j.issn1002-0306.20240302072024030207-4Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1Yongjiao GAO0Kun WANG1Jing ZHAO2Feng ZUO3College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaIn order to preliminarily explore the structure, immune and anti-aging activity of capsular polysaccharide (CPS) produced by Lactobacillus plantarum W1. In this study, CPS was extracted and prepared by enzymatic hydrolysis. The absolute molecular weight, functional groups and monosaccharide composition of CPS were analyzed by gel permeation chromatography system, Fourier transform infrared spectroscopy and high performance liquid chromatography. The immune and anti-aging activities of CPS were investigated using RAW264.7 cells and Caenorhabditis elegans (C. elegans) models. The results showed that CPS was a heteropolysaccharide with an average molecular weight of 5.49×104 Da, consisting of glucose and galactose in a molar ratio of 3.12:1. Furthermore, CPS had been found to enhance the proliferation and phagocytosis of RAW264.7 cells, as well as promote the secretion of NO and cytokines, thereby exerting an immunomodulatory effect. At a concentration of 125 μg/mL, the secretion levels of TNF-α, IL-6 and IL-1β in RAW264.7 cells reached their maximum values, which were 1.26 times, 1.11 times and 1.96 times higher than those in the control group, respectively. Concurrently, CPS significantly enhanced the antioxidant system of C. elegans (P<0.05), prolonged their lifespan, and improved their stress tolerance. The average lifespan of nematodes under normal, oxidative stress and heat shock conditions reached its maximum at 125 μg/mL, with lifespan extension rates of 21.05%±0.21%, 42.86%±0.27% and 50.00%±0.27%, respectively. These findings indicate that CPS exhibits significant biological activity and has the potential to be utilized as functional excipients in food products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030207capsular polysaccharidemacrophageimmune activitycaenorhabditis elegans |
| spellingShingle | Yongjiao GAO Kun WANG Jing ZHAO Feng ZUO Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 Shipin gongye ke-ji capsular polysaccharide macrophage immune activity caenorhabditis elegans |
| title | Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 |
| title_full | Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 |
| title_fullStr | Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 |
| title_full_unstemmed | Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 |
| title_short | Structure, Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1 |
| title_sort | structure immune and anti aging activities of capsular polysaccharide produced by lactobacillus plantarum w1 |
| topic | capsular polysaccharide macrophage immune activity caenorhabditis elegans |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030207 |
| work_keys_str_mv | AT yongjiaogao structureimmuneandantiagingactivitiesofcapsularpolysaccharideproducedbylactobacillusplantarumw1 AT kunwang structureimmuneandantiagingactivitiesofcapsularpolysaccharideproducedbylactobacillusplantarumw1 AT jingzhao structureimmuneandantiagingactivitiesofcapsularpolysaccharideproducedbylactobacillusplantarumw1 AT fengzuo structureimmuneandantiagingactivitiesofcapsularpolysaccharideproducedbylactobacillusplantarumw1 |