Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region
Background: There is very little published data on the antibody response to the Vi capsular polysaccharide (Vi-CPS) of S. Typhi during naturally acquired typhoid fever in an endemic area. Methodology: An indirect ELISA, using tyraminated, purified Vi-CPS, was used to assay anti-Vi-CPS antibodies fro...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2008-08-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/227 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148167617609728 |
|---|---|
| author | Deborah House Vo A. Ho To S. Diep Nguyen T. Chinh Phan V. Bay Ha Vinh Minh Duc Christopher M. Parry Gordon Dougan Nicholas J. White Jeremy J. Farrar Tran Tinh Hien John Wain |
| author_facet | Deborah House Vo A. Ho To S. Diep Nguyen T. Chinh Phan V. Bay Ha Vinh Minh Duc Christopher M. Parry Gordon Dougan Nicholas J. White Jeremy J. Farrar Tran Tinh Hien John Wain |
| author_sort | Deborah House |
| collection | DOAJ |
| description | Background: There is very little published data on the antibody response to the Vi capsular polysaccharide (Vi-CPS) of S. Typhi during naturally acquired typhoid fever in an endemic area.
Methodology: An indirect ELISA, using tyraminated, purified Vi-CPS, was used to assay anti-Vi-CPS antibodies from typhoid fever cases and controls living in the Ho Chi Minh City and Mekong Delta region of Viet Nam.
Results: Antibody response to Vi-CPS is significantly higher in typhoid patients who have been ill for more than two weeks than those who are in the first two weeks of illness. The anti-Vi-CPS response is similar for adults and children. Anti-Vi-CPS antibodies can be detected in the sera of non-typhoid patients. The frequency with which this occurs increases with age, probably reflecting increased exposure to S. Typhi.
Conclusions: Anti-Vi_CPS is elicited in persons infected with S. Typhi but only after a prolonged duration of illness. Vaccine trials have shown anti-Vi-CPS antibodies to be protective; thus early treatment of typhoid patients, i.e. in the first two week of illness before the Vi-CPS response is elicited, may inhibit the development of this protective immune response. |
| format | Article |
| id | doaj-art-7ac5f2f8a22b460a9cba0dd56fdc1d59 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-7ac5f2f8a22b460a9cba0dd56fdc1d592025-08-20T02:27:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-08-0120410.3855/jidc.227Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic regionDeborah House0Vo A. Ho1To S. Diep2Nguyen T. Chinh3Phan V. Bay4Ha Vinh5Minh Duc6Christopher M. Parry7Gordon Dougan8Nicholas J. White9Jeremy J. Farrar10Tran Tinh Hien11John Wain12Department of Biochemistry, Imperial College of Science, Technology and Medicine, LondonDong Thap Provincial Hospital, Cao Lanh, Dong Thap ProvinceHospital for Tropical Diseases, Ho Chi Minh CityDepartment of Infectious Diseases, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh CityDong Thap Provincial Hospital, Cao Lanh, Dong Thap ProvinceHospital for Tropical Diseases, Ho Chi Minh CityDong Thap Provincial Hospital, Cao Lanh, Dong Thap ProvinceCentre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, OxfordDepartment of Biochemistry, Imperial College of Science, Technology and Medicine, LondonCentre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, OxfordCentre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, OxfordHospital for Tropical Diseases, Ho Chi Minh CityCentre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, OxfordBackground: There is very little published data on the antibody response to the Vi capsular polysaccharide (Vi-CPS) of S. Typhi during naturally acquired typhoid fever in an endemic area. Methodology: An indirect ELISA, using tyraminated, purified Vi-CPS, was used to assay anti-Vi-CPS antibodies from typhoid fever cases and controls living in the Ho Chi Minh City and Mekong Delta region of Viet Nam. Results: Antibody response to Vi-CPS is significantly higher in typhoid patients who have been ill for more than two weeks than those who are in the first two weeks of illness. The anti-Vi-CPS response is similar for adults and children. Anti-Vi-CPS antibodies can be detected in the sera of non-typhoid patients. The frequency with which this occurs increases with age, probably reflecting increased exposure to S. Typhi. Conclusions: Anti-Vi_CPS is elicited in persons infected with S. Typhi but only after a prolonged duration of illness. Vaccine trials have shown anti-Vi-CPS antibodies to be protective; thus early treatment of typhoid patients, i.e. in the first two week of illness before the Vi-CPS response is elicited, may inhibit the development of this protective immune response.https://jidc.org/index.php/journal/article/view/227Vi capsuleSalmonella Typhityphoid feverantibodiesViet NamVietnam |
| spellingShingle | Deborah House Vo A. Ho To S. Diep Nguyen T. Chinh Phan V. Bay Ha Vinh Minh Duc Christopher M. Parry Gordon Dougan Nicholas J. White Jeremy J. Farrar Tran Tinh Hien John Wain Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region Journal of Infection in Developing Countries Vi capsule Salmonella Typhi typhoid fever antibodies Viet Nam Vietnam |
| title | Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| title_full | Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| title_fullStr | Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| title_full_unstemmed | Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| title_short | Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| title_sort | antibodies to the vi capsule of salmonella typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region |
| topic | Vi capsule Salmonella Typhi typhoid fever antibodies Viet Nam Vietnam |
| url | https://jidc.org/index.php/journal/article/view/227 |
| work_keys_str_mv | AT deborahhouse antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT voaho antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT tosdiep antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT nguyentchinh antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT phanvbay antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT havinh antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT minhduc antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT christophermparry antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT gordondougan antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT nicholasjwhite antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT jeremyjfarrar antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT trantinhhien antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion AT johnwain antibodiestothevicapsuleofsalmonellatyphiintheserumoftyphoidpatientsandhealthycontrolsubjectsfromatyphoidendemicregion |