Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max
In order to analyze the structure of leginsulin gene in leguminous plant, the leginsulin gene in soybean was amplified and cloneded from genomic DNA. Comparison of the DNA sequence with that of cDNA showed that the cloned fragment was 841 bp in size and contained one intron of 368 bp in length exist...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2000-01-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.01.0107 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849412718264582144 |
|---|---|
| author | TAN Jian-zhong LOU Cheng-fu HIRANO Hisashi |
| author_facet | TAN Jian-zhong LOU Cheng-fu HIRANO Hisashi |
| author_sort | TAN Jian-zhong |
| collection | DOAJ |
| description | In order to analyze the structure of leginsulin gene in leguminous plant, the leginsulin gene in soybean was amplified and cloneded from genomic DNA. Comparison of the DNA sequence with that of cDNA showed that the cloned fragment was 841 bp in size and contained one intron of 368 bp in length existed in the code region of signal peptide; there was one nucleotide difference in the non-code region of 3′ termination, but no difference in the code region of leginsulin between the genomic DNA and cDNA sequence. |
| format | Article |
| id | doaj-art-7abc09c16f0c4ef480483c2f23752abc |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-7abc09c16f0c4ef480483c2f23752abc2025-08-20T03:34:21ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552000-01-012610711010.3785/1008-9209.2000.01.010710089209Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine maxTAN Jian-zhongLOU Cheng-fuHIRANO HisashiIn order to analyze the structure of leginsulin gene in leguminous plant, the leginsulin gene in soybean was amplified and cloneded from genomic DNA. Comparison of the DNA sequence with that of cDNA showed that the cloned fragment was 841 bp in size and contained one intron of 368 bp in length existed in the code region of signal peptide; there was one nucleotide difference in the non-code region of 3′ termination, but no difference in the code region of leginsulin between the genomic DNA and cDNA sequence.https://www.academax.com/doi/10.3785/1008-9209.2000.01.0107soybeanleginsulinDNA sequencing |
| spellingShingle | TAN Jian-zhong LOU Cheng-fu HIRANO Hisashi Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max 浙江大学学报. 农业与生命科学版 soybean leginsulin DNA sequencing |
| title | Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max |
| title_full | Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max |
| title_fullStr | Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max |
| title_full_unstemmed | Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max |
| title_short | Cloning and sequencing of special seed protein (Leginsulin) gene in soybean Mlycine max |
| title_sort | cloning and sequencing of special seed protein leginsulin gene in soybean mlycine max |
| topic | soybean leginsulin DNA sequencing |
| url | https://www.academax.com/doi/10.3785/1008-9209.2000.01.0107 |
| work_keys_str_mv | AT tanjianzhong cloningandsequencingofspecialseedproteinleginsulingeneinsoybeanmlycinemax AT louchengfu cloningandsequencingofspecialseedproteinleginsulingeneinsoybeanmlycinemax AT hiranohisashi cloningandsequencingofspecialseedproteinleginsulingeneinsoybeanmlycinemax |