Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1
Background Acute kidney injury (AKI) is a common complication of cardiac surgery. Human umbilical cord mesenchymal stem cell (hUCMSC)-derived exosomes affect tissue damage and repair, and miR-21-5p reduces apoptosis and inflammation. However, miR-21-5p’s role in cardiac surgery remains unclear.Metho...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2537797 |
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| author | Zhengmin Li Fang He Shuyang Dai Qingqing Yu Chenchen Si Fan Wu Wenxin Zhao Biyao Zhang Poshi Xu |
| author_facet | Zhengmin Li Fang He Shuyang Dai Qingqing Yu Chenchen Si Fan Wu Wenxin Zhao Biyao Zhang Poshi Xu |
| author_sort | Zhengmin Li |
| collection | DOAJ |
| description | Background Acute kidney injury (AKI) is a common complication of cardiac surgery. Human umbilical cord mesenchymal stem cell (hUCMSC)-derived exosomes affect tissue damage and repair, and miR-21-5p reduces apoptosis and inflammation. However, miR-21-5p’s role in cardiac surgery remains unclear.Methods Kidney injury molecule (KIM-1), neutrophil gelatinase-associated lipid carrier protein (NGAL), serum creatinine, and blood urea nitrogen (BUN) levels were tested by enzyme-linked immunosorbent assay. hUCMSCs and exosomes were identified via Oil Red O staining kit, alkaline phosphatase staining kit, flow cytometry, transmission electron microscopy, Western blot, and immunofluorescence. Cell viability and apoptosis were detected by cell counting kit 8 and flow cytometry. Interleukin-1β, interleukin-6, and tumor necrosis factor-alpha levels were tested by ELISA kits. Relativity between miR-21-5p and TEA domain family member 1 (TEAD1) was verified by dual-luciferase reporter gene assay and western blot. TEAD1, BUN, and creatinine levels in vivo were tested via immunohistochemistry and automatic biochemistry analyzer.Results KIM-1, NGAL, serum creatinine, and BUN levels were increased and miR-21-5p expression was inhibited in AKI after cardiac surgery. In AKI after cardiac surgery, hUCMSCs-Exos inhibited apoptosis and pyroptosis and promoted viability via miR-21-5p regulation. hUCMSCs-Exos retarded AKI after cardiac surgery progression by facilitating miR-21-5p targeting of TEAD1. hUCMSCs-Exos promoted miR-21-5p and inhibited TEAD1 expression in vivo. miR-21-5p knockdown facilitated TEAD1 expression in vivo. Results were exactly the same in vivo and in vitro AKI models.Conclusions hUCMSCs-Exos protect against AKI by enhancing miR-21-5p/TEAD1 binding in vivo and in vitro. |
| format | Article |
| id | doaj-art-7ababf72c6f848e38bc3dceb0e6af677 |
| institution | Kabale University |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-7ababf72c6f848e38bc3dceb0e6af6772025-08-20T03:39:18ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492025-12-0147110.1080/0886022X.2025.2537797Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1Zhengmin Li0Fang He1Shuyang Dai2Qingqing Yu3Chenchen Si4Fan Wu5Wenxin Zhao6Biyao Zhang7Poshi Xu8Department of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaOperating Room, Woman & Infants Hospital of Zhengzhou, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaDepartment of Medical Laboratory, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, ChinaBackground Acute kidney injury (AKI) is a common complication of cardiac surgery. Human umbilical cord mesenchymal stem cell (hUCMSC)-derived exosomes affect tissue damage and repair, and miR-21-5p reduces apoptosis and inflammation. However, miR-21-5p’s role in cardiac surgery remains unclear.Methods Kidney injury molecule (KIM-1), neutrophil gelatinase-associated lipid carrier protein (NGAL), serum creatinine, and blood urea nitrogen (BUN) levels were tested by enzyme-linked immunosorbent assay. hUCMSCs and exosomes were identified via Oil Red O staining kit, alkaline phosphatase staining kit, flow cytometry, transmission electron microscopy, Western blot, and immunofluorescence. Cell viability and apoptosis were detected by cell counting kit 8 and flow cytometry. Interleukin-1β, interleukin-6, and tumor necrosis factor-alpha levels were tested by ELISA kits. Relativity between miR-21-5p and TEA domain family member 1 (TEAD1) was verified by dual-luciferase reporter gene assay and western blot. TEAD1, BUN, and creatinine levels in vivo were tested via immunohistochemistry and automatic biochemistry analyzer.Results KIM-1, NGAL, serum creatinine, and BUN levels were increased and miR-21-5p expression was inhibited in AKI after cardiac surgery. In AKI after cardiac surgery, hUCMSCs-Exos inhibited apoptosis and pyroptosis and promoted viability via miR-21-5p regulation. hUCMSCs-Exos retarded AKI after cardiac surgery progression by facilitating miR-21-5p targeting of TEAD1. hUCMSCs-Exos promoted miR-21-5p and inhibited TEAD1 expression in vivo. miR-21-5p knockdown facilitated TEAD1 expression in vivo. Results were exactly the same in vivo and in vitro AKI models.Conclusions hUCMSCs-Exos protect against AKI by enhancing miR-21-5p/TEAD1 binding in vivo and in vitro.https://www.tandfonline.com/doi/10.1080/0886022X.2025.2537797Human umbilical cord mesenchymal stem cellsexosomesmiR-21-5pTEA domain family member 1acute kidney injury |
| spellingShingle | Zhengmin Li Fang He Shuyang Dai Qingqing Yu Chenchen Si Fan Wu Wenxin Zhao Biyao Zhang Poshi Xu Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 Renal Failure Human umbilical cord mesenchymal stem cells exosomes miR-21-5p TEA domain family member 1 acute kidney injury |
| title | Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 |
| title_full | Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 |
| title_fullStr | Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 |
| title_full_unstemmed | Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 |
| title_short | Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1 |
| title_sort | exosomes derived from human umbilical cord mesenchymal stem cells ameliorate aki after cardiac surgery by facilitating mir 21 5p targeting tead1 |
| topic | Human umbilical cord mesenchymal stem cells exosomes miR-21-5p TEA domain family member 1 acute kidney injury |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2537797 |
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