Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery
ABSTRACT Vanillin is the most in‐demand flavouring compound in the world and because vanillin extracted from vanilla pods cannot meet the global demand, most vanillin on the market today is chemically synthesised. Increasing demands by consumers for natural ingredients have inspired efforts to devel...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Microbial Biotechnology |
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| Online Access: | https://doi.org/10.1111/1751-7915.70152 |
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| author | Ilona A. Ruhl Sean P. Woodworth Stefan J. Haugen Hannah M. Alt Gregg T. Beckham Christopher W. Johnson |
| author_facet | Ilona A. Ruhl Sean P. Woodworth Stefan J. Haugen Hannah M. Alt Gregg T. Beckham Christopher W. Johnson |
| author_sort | Ilona A. Ruhl |
| collection | DOAJ |
| description | ABSTRACT Vanillin is the most in‐demand flavouring compound in the world and because vanillin extracted from vanilla pods cannot meet the global demand, most vanillin on the market today is chemically synthesised. Increasing demands by consumers for natural ingredients have inspired efforts to develop vanillin derived from microbial sources. These efforts have been challenged by low titers, likely caused by the toxicity of vanillin to most microbial biocatalysts. In this study, we engineered a Pseudomonas putida KT2440‐derived strain that accumulated vanillin from ferulic acid to 0.64 g/L. To increase the overall titre, we applied a hydrophobic polystyrene‐based resin to vanillin‐accumulating cultures, which enabled an increase in total vanillin recovery to an apparent titre of 3.35 g/L. This study demonstrates that P. putida can accumulate vanillin from ferulic acid to higher titers when vanillin is removed from the cultivation medium, mitigating its toxicity. |
| format | Article |
| id | doaj-art-7ab58a545abf47b896f2c4742bbb3c43 |
| institution | OA Journals |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj-art-7ab58a545abf47b896f2c4742bbb3c432025-08-20T01:57:01ZengWileyMicrobial Biotechnology1751-79152025-05-01185n/an/a10.1111/1751-7915.70152Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product RecoveryIlona A. Ruhl0Sean P. Woodworth1Stefan J. Haugen2Hannah M. Alt3Gregg T. Beckham4Christopher W. Johnson5Renewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USARenewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USARenewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USARenewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USARenewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USARenewable Resources and Enabling Sciences Center National Renewable Energy Laboratory Golden Colorado USAABSTRACT Vanillin is the most in‐demand flavouring compound in the world and because vanillin extracted from vanilla pods cannot meet the global demand, most vanillin on the market today is chemically synthesised. Increasing demands by consumers for natural ingredients have inspired efforts to develop vanillin derived from microbial sources. These efforts have been challenged by low titers, likely caused by the toxicity of vanillin to most microbial biocatalysts. In this study, we engineered a Pseudomonas putida KT2440‐derived strain that accumulated vanillin from ferulic acid to 0.64 g/L. To increase the overall titre, we applied a hydrophobic polystyrene‐based resin to vanillin‐accumulating cultures, which enabled an increase in total vanillin recovery to an apparent titre of 3.35 g/L. This study demonstrates that P. putida can accumulate vanillin from ferulic acid to higher titers when vanillin is removed from the cultivation medium, mitigating its toxicity.https://doi.org/10.1111/1751-7915.70152bioconversionferulic acidin situ product recoverymetabolic engineeringPseudomonas putida KT2440vanillin |
| spellingShingle | Ilona A. Ruhl Sean P. Woodworth Stefan J. Haugen Hannah M. Alt Gregg T. Beckham Christopher W. Johnson Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery Microbial Biotechnology bioconversion ferulic acid in situ product recovery metabolic engineering Pseudomonas putida KT2440 vanillin |
| title | Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery |
| title_full | Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery |
| title_fullStr | Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery |
| title_full_unstemmed | Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery |
| title_short | Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery |
| title_sort | production of vanillin from ferulic acid by pseudomonas putida kt2440 using metabolic engineering and in situ product recovery |
| topic | bioconversion ferulic acid in situ product recovery metabolic engineering Pseudomonas putida KT2440 vanillin |
| url | https://doi.org/10.1111/1751-7915.70152 |
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