Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability
Bioremediation is a biological treatment process that uses microorganisms to biodegrade environmental pollutants. In this study, lipase production, emulsification of hydrocarbons, growth in the presence of inhibitors, and decolorization of triphenylmethane dyes by the selected isolate were tested....
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Biotechnology Society of Nepal
2025-07-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://nepjb.com/index.php/NJB/article/view/376 |
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| author | Asmita Shrestha Gyanu Raj Pandey Sudip Silwal Raj Kumar Shrestha Dinesh Tiwari |
| author_facet | Asmita Shrestha Gyanu Raj Pandey Sudip Silwal Raj Kumar Shrestha Dinesh Tiwari |
| author_sort | Asmita Shrestha |
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Bioremediation is a biological treatment process that uses microorganisms to biodegrade environmental pollutants. In this study, lipase production, emulsification of hydrocarbons, growth in the presence of inhibitors, and decolorization of triphenylmethane dyes by the selected isolate were tested. Lipase production was confirmed by the development of a halo region around the colonies on tributyrin agar by the isolate. The isolate was identified as Staphylococcus saprophyticus by 16S rRNA gene sequencing and biochemical features. Among different hydrocarbons used, the maximum emulsification index was found on xylene (83.33 ± 3.33) % and minimum on diesel (26.67 ±3.33) %. The isolate was able to decolorize malachite green, phenol red, fuchsin, and crystal violet with maximum decolorization was observed for malachite green (96.53 ± 0.69) % and minimum with crystal violet (27.04 ± 1.13) %. The isolate could grow in the presence of growth inhibitors like phenol and lead acetate but was unable to grow in the presence of mercuric chloride. This study suggests that the identified isolate, Staphylococcus saprophyticus Li-B5, is suitable for bioremediation because it can emulsify different hydrocarbons, decolorize various dyes, and produce lipase enzymes.
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| format | Article |
| id | doaj-art-7ab1096daba54b37bbf46a49150345ea |
| institution | Kabale University |
| issn | 2091-1130 2467-9313 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Biotechnology Society of Nepal |
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| series | Nepal Journal of Biotechnology |
| spelling | doaj-art-7ab1096daba54b37bbf46a49150345ea2025-08-20T04:00:55ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132025-07-0113110.54796/njb.v13i1.376Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation ApplicabilityAsmita Shrestha0https://orcid.org/0000-0001-7823-4913Gyanu Raj Pandey1https://orcid.org/0000-0003-0400-460XSudip Silwal2https://orcid.org/0000-0002-0493-0024Raj Kumar Shrestha3https://orcid.org/0000-0001-6893-6962Dinesh Tiwari4https://orcid.org/0009-0006-5927-6329Shubham Biotech Nepal Pvt. Ltd., Bharatpur-29, Chitwan, 44200, Nepal; Biotechnological Research and Development Center Pvt. Ltd., Bharatpur-29, Chitwan, 44200, NepalShubham Biotech Nepal Pvt. Ltd., Bharatpur-29, Chitwan, 44200, Nepal; Biotechnological Research and Development Center Pvt. Ltd., Bharatpur-29, Chitwan, 44200, NepalShubham Biotech Nepal Pvt. Ltd., Bharatpur-29, Chitwan, 44200, NepalShubham Biotech Nepal Pvt. Ltd., Bharatpur-29, Chitwan, 44200, NepalShubham Biotech Nepal Pvt. Ltd., Bharatpur-29, Chitwan, 44200, Nepal Bioremediation is a biological treatment process that uses microorganisms to biodegrade environmental pollutants. In this study, lipase production, emulsification of hydrocarbons, growth in the presence of inhibitors, and decolorization of triphenylmethane dyes by the selected isolate were tested. Lipase production was confirmed by the development of a halo region around the colonies on tributyrin agar by the isolate. The isolate was identified as Staphylococcus saprophyticus by 16S rRNA gene sequencing and biochemical features. Among different hydrocarbons used, the maximum emulsification index was found on xylene (83.33 ± 3.33) % and minimum on diesel (26.67 ±3.33) %. The isolate was able to decolorize malachite green, phenol red, fuchsin, and crystal violet with maximum decolorization was observed for malachite green (96.53 ± 0.69) % and minimum with crystal violet (27.04 ± 1.13) %. The isolate could grow in the presence of growth inhibitors like phenol and lead acetate but was unable to grow in the presence of mercuric chloride. This study suggests that the identified isolate, Staphylococcus saprophyticus Li-B5, is suitable for bioremediation because it can emulsify different hydrocarbons, decolorize various dyes, and produce lipase enzymes. https://nepjb.com/index.php/NJB/article/view/376BioremediationStaphylococcus saprophyticusBioemulsifiersLipaseTriphenylmethane dyes |
| spellingShingle | Asmita Shrestha Gyanu Raj Pandey Sudip Silwal Raj Kumar Shrestha Dinesh Tiwari Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability Nepal Journal of Biotechnology Bioremediation Staphylococcus saprophyticus Bioemulsifiers Lipase Triphenylmethane dyes |
| title | Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability |
| title_full | Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability |
| title_fullStr | Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability |
| title_full_unstemmed | Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability |
| title_short | Identification of Lipase Producing Staphylococcus saprophyticus Li-B5 with Potential Bioremediation Applicability |
| title_sort | identification of lipase producing staphylococcus saprophyticus li b5 with potential bioremediation applicability |
| topic | Bioremediation Staphylococcus saprophyticus Bioemulsifiers Lipase Triphenylmethane dyes |
| url | https://nepjb.com/index.php/NJB/article/view/376 |
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