Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods

The development of highly sensitive and selective Surface-Enhanced Raman Spectroscopy (SERS) substrates is crucial for biomedical applications. This study presents an advanced SERS substrate by integrating silver nanoparticles (AgNPs) with ZnO nanorods (NRs), leveraging hot electron dynamics to enha...

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Main Authors: N.A. Abdullah, M.Z.A. Razak, T.H.T. Aziz, A.R.M. Zain, M.S.M. Jamil, M.M. Salleh
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Results in Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211715625003698
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author N.A. Abdullah
M.Z.A. Razak
T.H.T. Aziz
A.R.M. Zain
M.S.M. Jamil
M.M. Salleh
author_facet N.A. Abdullah
M.Z.A. Razak
T.H.T. Aziz
A.R.M. Zain
M.S.M. Jamil
M.M. Salleh
author_sort N.A. Abdullah
collection DOAJ
description The development of highly sensitive and selective Surface-Enhanced Raman Spectroscopy (SERS) substrates is crucial for biomedical applications. This study presents an advanced SERS substrate by integrating silver nanoparticles (AgNPs) with ZnO nanorods (NRs), leveraging hot electron dynamics to enhance the SERS signal for non-SERS-active creatinine detection. AgNPs were deposited onto ZnO NRs using silver nitrate (AgNO₃) concentrations ranging from 0.01 M to 0.16 M, resulting in distinct nanoparticle distributions optimized for hot electron generation. The structural and optical properties of the substrates were systematically analyzed using FESEM, EDX mapping, and UV–Vis spectroscopy. The AgZnO nanorods synthesized with 0.13 M AgNO₃ demonstrated the most favorable AgNPs distribution, achieving a high-density configuration that significantly enhanced the Raman signal, yielding an enhancement factor (EF) of 5.07 × 103 for 1.0 × 10−2 M non-SERS-active creatinine molecules, representing a 56.90 % improvement in sensitivity. This enhancement is attributed to the synergistic effect of localized surface plasmon resonance (LSPR) and efficient hot electron transfer, facilitated by the optimized AgNPs distribution. Moreover, the substrate showed strong reproducibility (RSD < 30 %), short-term stability over five days, and uniform SERS activity across the surface. These findings establish a direct correlation between AgNPs arrangement and hot electron-driven SERS enhancement, highlighting the potential of AgZnO nanorods as a highly effective SERS substrate for biomedical applications.
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spelling doaj-art-7aab5bfc36f144ef8429657c6a4c76df2025-08-20T03:39:09ZengElsevierResults in Chemistry2211-71562025-07-011610238610.1016/j.rechem.2025.102386Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorodsN.A. Abdullah0M.Z.A. Razak1T.H.T. Aziz2A.R.M. Zain3M.S.M. Jamil4M.M. Salleh5Institute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaInstitute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaCorresponding author.; Institute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaInstitute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaInstitute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaInstitute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bandar Baru Bangi, Selangor, MalaysiaThe development of highly sensitive and selective Surface-Enhanced Raman Spectroscopy (SERS) substrates is crucial for biomedical applications. This study presents an advanced SERS substrate by integrating silver nanoparticles (AgNPs) with ZnO nanorods (NRs), leveraging hot electron dynamics to enhance the SERS signal for non-SERS-active creatinine detection. AgNPs were deposited onto ZnO NRs using silver nitrate (AgNO₃) concentrations ranging from 0.01 M to 0.16 M, resulting in distinct nanoparticle distributions optimized for hot electron generation. The structural and optical properties of the substrates were systematically analyzed using FESEM, EDX mapping, and UV–Vis spectroscopy. The AgZnO nanorods synthesized with 0.13 M AgNO₃ demonstrated the most favorable AgNPs distribution, achieving a high-density configuration that significantly enhanced the Raman signal, yielding an enhancement factor (EF) of 5.07 × 103 for 1.0 × 10−2 M non-SERS-active creatinine molecules, representing a 56.90 % improvement in sensitivity. This enhancement is attributed to the synergistic effect of localized surface plasmon resonance (LSPR) and efficient hot electron transfer, facilitated by the optimized AgNPs distribution. Moreover, the substrate showed strong reproducibility (RSD < 30 %), short-term stability over five days, and uniform SERS activity across the surface. These findings establish a direct correlation between AgNPs arrangement and hot electron-driven SERS enhancement, highlighting the potential of AgZnO nanorods as a highly effective SERS substrate for biomedical applications.http://www.sciencedirect.com/science/article/pii/S2211715625003698AgNPs distribution densityAgZnO nanorodsHot electrons generationCreatinine detectionNon-SERS-active molecule
spellingShingle N.A. Abdullah
M.Z.A. Razak
T.H.T. Aziz
A.R.M. Zain
M.S.M. Jamil
M.M. Salleh
Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
Results in Chemistry
AgNPs distribution density
AgZnO nanorods
Hot electrons generation
Creatinine detection
Non-SERS-active molecule
title Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
title_full Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
title_fullStr Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
title_full_unstemmed Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
title_short Hot electron-driven SERS enhancement of non-SERS-active creatinine using Ag-decorated ZnO nanorods
title_sort hot electron driven sers enhancement of non sers active creatinine using ag decorated zno nanorods
topic AgNPs distribution density
AgZnO nanorods
Hot electrons generation
Creatinine detection
Non-SERS-active molecule
url http://www.sciencedirect.com/science/article/pii/S2211715625003698
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