Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing

Abstract Antibacterial therapy targeting the regulation of macrophage polarization may be a useful approach for normalizing the immune environment and accelerating wound healing. Inspired by black phosphorus‐based nanoplatforms, more stable yet less‐explored violet phosphorus nanosheets (VPNSs) are...

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Main Authors: Zhuo Dai, Qiang Li, Meng Dang, Xiaoye Li, Ao He, Weijun Xiu, Minjin Wang, Yu Zhang, Meng Ding, Heng Dong, Yongbin Mou
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202407545
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author Zhuo Dai
Qiang Li
Meng Dang
Xiaoye Li
Ao He
Weijun Xiu
Minjin Wang
Yu Zhang
Meng Ding
Heng Dong
Yongbin Mou
author_facet Zhuo Dai
Qiang Li
Meng Dang
Xiaoye Li
Ao He
Weijun Xiu
Minjin Wang
Yu Zhang
Meng Ding
Heng Dong
Yongbin Mou
author_sort Zhuo Dai
collection DOAJ
description Abstract Antibacterial therapy targeting the regulation of macrophage polarization may be a useful approach for normalizing the immune environment and accelerating wound healing. Inspired by black phosphorus‐based nanoplatforms, more stable yet less‐explored violet phosphorus nanosheets (VPNSs) are expected to provide a superior solution for effectively combating bacterial infections. In this study, an average thickness of 5–7 nm VPNSs are fabricated through the liquid‐phase exfoliation method to serve as an immunoregulatory dressing for the treatment of infected wounds. VPNSs attenuated excessive reactive oxygen species (ROS) and reduced the accumulation of proinflammatory M1 macrophages, showing notable antioxidant and anti‐inflammatory properties. Comprehensive RNA sequencing further elucidated the potential immunoregulatory mechanisms of VPNSs, including modulation of the inflammatory response and enzyme regulator activity. Additionally, the inherent photothermal properties of the VPNSs contributed significantly to their antibacterial efficacy. When combined with near‐infrared laser irradiation, VPNSs showed remarkable effectiveness in reducing infection‐related complications and expediting wound healing in infected skin wound models. The rapid promotion of wound healing through ROS clearance, the regulation of macrophage polarization, and hyperthermia generation underscores the potential of the violet‐phosphorus‐based nanoplatforms as clinically viable agents for treating infected wounds. This study suggests that VPNSs are promising candidates for clinical anti‐infective and anti‐inflammatory applications.
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spelling doaj-art-7aa034a99fde4e868a742c8347f583652025-08-20T02:50:00ZengWileyAdvanced Science2198-38442024-12-011146n/an/a10.1002/advs.202407545Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound HealingZhuo Dai0Qiang Li1Meng Dang2Xiaoye Li3Ao He4Weijun Xiu5Minjin Wang6Yu Zhang7Meng Ding8Heng Dong9Yongbin Mou10Nanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaInstitute for Health Innovation and Technology Biomedical Engineering Department National University of Singapore 21 Lower Kent Ridge Road Singapore 119276 SingaporeNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaNanjing Stomatological Hospital, Affiliated Hospital of Medical School Institute of Stomatology Nanjing University 30 Zhongyang Road Nanjing Jiangsu 210008 ChinaAbstract Antibacterial therapy targeting the regulation of macrophage polarization may be a useful approach for normalizing the immune environment and accelerating wound healing. Inspired by black phosphorus‐based nanoplatforms, more stable yet less‐explored violet phosphorus nanosheets (VPNSs) are expected to provide a superior solution for effectively combating bacterial infections. In this study, an average thickness of 5–7 nm VPNSs are fabricated through the liquid‐phase exfoliation method to serve as an immunoregulatory dressing for the treatment of infected wounds. VPNSs attenuated excessive reactive oxygen species (ROS) and reduced the accumulation of proinflammatory M1 macrophages, showing notable antioxidant and anti‐inflammatory properties. Comprehensive RNA sequencing further elucidated the potential immunoregulatory mechanisms of VPNSs, including modulation of the inflammatory response and enzyme regulator activity. Additionally, the inherent photothermal properties of the VPNSs contributed significantly to their antibacterial efficacy. When combined with near‐infrared laser irradiation, VPNSs showed remarkable effectiveness in reducing infection‐related complications and expediting wound healing in infected skin wound models. The rapid promotion of wound healing through ROS clearance, the regulation of macrophage polarization, and hyperthermia generation underscores the potential of the violet‐phosphorus‐based nanoplatforms as clinically viable agents for treating infected wounds. This study suggests that VPNSs are promising candidates for clinical anti‐infective and anti‐inflammatory applications.https://doi.org/10.1002/advs.202407545antibacterialantioxidantmacrophage polarizationviolet phosphorus nanosheetswound healing
spellingShingle Zhuo Dai
Qiang Li
Meng Dang
Xiaoye Li
Ao He
Weijun Xiu
Minjin Wang
Yu Zhang
Meng Ding
Heng Dong
Yongbin Mou
Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
Advanced Science
antibacterial
antioxidant
macrophage polarization
violet phosphorus nanosheets
wound healing
title Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
title_full Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
title_fullStr Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
title_full_unstemmed Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
title_short Radical‐Scavenging Violet Phosphorus Nanosheets for Attenuating Hyperinflammation and Promoting Infected Wound Healing
title_sort radical scavenging violet phosphorus nanosheets for attenuating hyperinflammation and promoting infected wound healing
topic antibacterial
antioxidant
macrophage polarization
violet phosphorus nanosheets
wound healing
url https://doi.org/10.1002/advs.202407545
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