Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke
The involvement of Group 3 innate lymphoid cells (ILC3s) and dendritic cells (DCs) in chronic lung inflammation has been increasingly regarded as the key to understand the inflammatory mechanisms of smoke-related chronic obstructive pulmonary disease (COPD). However, the mechanism underlying the eng...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | COPD |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15412555.2024.2389909 |
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| author | Caixia Liang Ying Shen Yifang Xu Yi Liang Shilin Qiu Haijuan Tang Xiaoning Zhong |
| author_facet | Caixia Liang Ying Shen Yifang Xu Yi Liang Shilin Qiu Haijuan Tang Xiaoning Zhong |
| author_sort | Caixia Liang |
| collection | DOAJ |
| description | The involvement of Group 3 innate lymphoid cells (ILC3s) and dendritic cells (DCs) in chronic lung inflammation has been increasingly regarded as the key to understand the inflammatory mechanisms of smoke-related chronic obstructive pulmonary disease (COPD). However, the mechanism underlying the engagement of both remains unclear. Our study aimed to explore NCR−ILC3 differentiation in the lungs of mice exposed to cigarette smoke (CS) and to further investigate whether DCs activated by CS exposure contribute to the differentiation of ILCs into NCR−ILC3s. The study involved both in vivo and in vitro experiments. In the former, the frequencies of lung NCR−ILC3s and NKp46−IL-17A+ ILCs and the expression of DCs, CD40, CD86, IL-23, and IL-1β quantified by flow cytometry were compared between CS-exposed mice and air-exposed mice. In the latter, NKp46−IL-17A+ ILC frequencies quantified by flow cytometry were compared after two cocultures, one involving lung CD45+Lin−CD127+ ILCs sorted from air-exposed mice and DCs sifted by CD11c magnetic beads from CS-exposed mice and another including identical CD45+Lin−CD127+ ILCs and DCs from air-exposed mice. The results indicated significant increases in the frequencies of NCR−ILC3s and NKp46−IL-17A+ ILCs; in the expression of DCs, CD40, CD86, IL-23, and IL-1β in CS-exposed mice; and in the frequency of NKp46−IL-17A+ ILCs after the coculture with DCs from CS-exposed mice. In conclusion, CS exposure increases the frequency of lung ILCs and NCR−ILC3s. CS-induced DC activation enhances the differentiation of ILCs into NCR−ILC3s, which likely acts as a mediating step in the involvement of NCR-ILC3s in chronic lung inflammation. |
| format | Article |
| id | doaj-art-7a9d4202bc224ccd8a584783ab149ef6 |
| institution | OA Journals |
| issn | 1541-2555 1541-2563 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | COPD |
| spelling | doaj-art-7a9d4202bc224ccd8a584783ab149ef62025-08-20T01:57:20ZengTaylor & Francis GroupCOPD1541-25551541-25632024-12-0121110.1080/15412555.2024.2389909Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette SmokeCaixia Liang0Ying Shen1Yifang Xu2Yi Liang3Shilin Qiu4Haijuan Tang5Xiaoning Zhong6Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaGeneral Practice School, Guangxi Medical University, Nanning, P.R. ChinaDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. ChinaThe involvement of Group 3 innate lymphoid cells (ILC3s) and dendritic cells (DCs) in chronic lung inflammation has been increasingly regarded as the key to understand the inflammatory mechanisms of smoke-related chronic obstructive pulmonary disease (COPD). However, the mechanism underlying the engagement of both remains unclear. Our study aimed to explore NCR−ILC3 differentiation in the lungs of mice exposed to cigarette smoke (CS) and to further investigate whether DCs activated by CS exposure contribute to the differentiation of ILCs into NCR−ILC3s. The study involved both in vivo and in vitro experiments. In the former, the frequencies of lung NCR−ILC3s and NKp46−IL-17A+ ILCs and the expression of DCs, CD40, CD86, IL-23, and IL-1β quantified by flow cytometry were compared between CS-exposed mice and air-exposed mice. In the latter, NKp46−IL-17A+ ILC frequencies quantified by flow cytometry were compared after two cocultures, one involving lung CD45+Lin−CD127+ ILCs sorted from air-exposed mice and DCs sifted by CD11c magnetic beads from CS-exposed mice and another including identical CD45+Lin−CD127+ ILCs and DCs from air-exposed mice. The results indicated significant increases in the frequencies of NCR−ILC3s and NKp46−IL-17A+ ILCs; in the expression of DCs, CD40, CD86, IL-23, and IL-1β in CS-exposed mice; and in the frequency of NKp46−IL-17A+ ILCs after the coculture with DCs from CS-exposed mice. In conclusion, CS exposure increases the frequency of lung ILCs and NCR−ILC3s. CS-induced DC activation enhances the differentiation of ILCs into NCR−ILC3s, which likely acts as a mediating step in the involvement of NCR-ILC3s in chronic lung inflammation.https://www.tandfonline.com/doi/10.1080/15412555.2024.2389909Cigarette smoke exposuredendritic cellsILCsNCR−ILC3sIL-17a |
| spellingShingle | Caixia Liang Ying Shen Yifang Xu Yi Liang Shilin Qiu Haijuan Tang Xiaoning Zhong Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke COPD Cigarette smoke exposure dendritic cells ILCs NCR−ILC3s IL-17a |
| title | Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke |
| title_full | Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke |
| title_fullStr | Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke |
| title_full_unstemmed | Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke |
| title_short | Dendritic Cells Promote the Differentiation of ILCs into NCR−ILC3s in the Lungs of Mice Exposed to Cigarette Smoke |
| title_sort | dendritic cells promote the differentiation of ilcs into ncr ilc3s in the lungs of mice exposed to cigarette smoke |
| topic | Cigarette smoke exposure dendritic cells ILCs NCR−ILC3s IL-17a |
| url | https://www.tandfonline.com/doi/10.1080/15412555.2024.2389909 |
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