Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro

Dengue virus (DENV) infection is still a global health problem. The severity of DENV infection is related to the high viral load and cytokine storm caused by excessive inflammation. There is no specific antiviral used for DENV. Meanwhile, the use of anti-inflammatory drugs for DENV is limited to pat...

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Main Authors: Regita Aulia Rosalina, Beti Ernawati Dewi, Fithriyah Fithriyah
Format: Article
Language:English
Published: Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology 2025-03-01
Series:Journal of Advanced Biotechnology and Experimental Therapeutics
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Online Access:https://www.bsmiab.org/jabet/?mno=214390
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author Regita Aulia Rosalina
Beti Ernawati Dewi
Fithriyah Fithriyah
author_facet Regita Aulia Rosalina
Beti Ernawati Dewi
Fithriyah Fithriyah
author_sort Regita Aulia Rosalina
collection DOAJ
description Dengue virus (DENV) infection is still a global health problem. The severity of DENV infection is related to the high viral load and cytokine storm caused by excessive inflammation. There is no specific antiviral used for DENV. Meanwhile, the use of anti-inflammatory drugs for DENV is limited to patients with severe clinical symptoms. Interestingly, favipiravir (FVP) and quinine sulfate (QS) have been reported as repurposing drugs that can inhibit DENV replication. However, their anti-inflammatory activity in DENV infection has not been studied yet. Thus, the current study aimed to evaluate both antiviral and inflammatory activities of FVP and QS in Vero and PBMC cells. The FVP and QS antiviral activities were analyzed through half-maximal inhibitory concentration (IC50) and half-maximal cytotoxicity concentration (CC50) values against DENV serotype-1 on Vero cells. The anti-inflammatory activities of FVP and QS were measured by the relative expression of TNF-α, IL-6, IL-10 cytokines, and the transcription factor NF-κB from DENV-1 infected peripheral blood mononuclear cells (PBMC) in vitro. The results showed that the IC50, CC50, and selectivity index (SI) for FVP were 2.72 μg/mL, 156.78 μg/mL, and 58, respectively. Meanwhile, the IC50, CC50, and SI of QS were 14.97 μg/mL, 85.2 μg/mL, and 5.69. Also, FVP and QS reduced the expression of IL-6 and IL-10 but induced the expression of TNF-α, and the transcription factor NF-κB in PBMC with the presence of antibody-dependent enhancement (ADE). Further, FVP has better DENV-1 antiviral activities compared to QS. However, in comparison with QS, FVP showed lower anti-inflammatory activities. Further studies are needed to explore the antiviral and anti-inflammatory mechanism of FVP and QS in the DENV-infected models. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 150-162]
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spelling doaj-art-7a84c57af853416788f9180af5b343722025-01-30T13:01:08ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602025-03-018115016210.5455/jabet.2025.13214390Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitroRegita Aulia Rosalina0Beti Ernawati Dewi1Fithriyah Fithriyah2Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Salemba Raya No. 6, Jakarta, 10430, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Pegangsaan Timur No. 16, Jakarta, 10320, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Pegangsaan Timur No. 16, Jakarta, 10320, IndonesiaDengue virus (DENV) infection is still a global health problem. The severity of DENV infection is related to the high viral load and cytokine storm caused by excessive inflammation. There is no specific antiviral used for DENV. Meanwhile, the use of anti-inflammatory drugs for DENV is limited to patients with severe clinical symptoms. Interestingly, favipiravir (FVP) and quinine sulfate (QS) have been reported as repurposing drugs that can inhibit DENV replication. However, their anti-inflammatory activity in DENV infection has not been studied yet. Thus, the current study aimed to evaluate both antiviral and inflammatory activities of FVP and QS in Vero and PBMC cells. The FVP and QS antiviral activities were analyzed through half-maximal inhibitory concentration (IC50) and half-maximal cytotoxicity concentration (CC50) values against DENV serotype-1 on Vero cells. The anti-inflammatory activities of FVP and QS were measured by the relative expression of TNF-α, IL-6, IL-10 cytokines, and the transcription factor NF-κB from DENV-1 infected peripheral blood mononuclear cells (PBMC) in vitro. The results showed that the IC50, CC50, and selectivity index (SI) for FVP were 2.72 μg/mL, 156.78 μg/mL, and 58, respectively. Meanwhile, the IC50, CC50, and SI of QS were 14.97 μg/mL, 85.2 μg/mL, and 5.69. Also, FVP and QS reduced the expression of IL-6 and IL-10 but induced the expression of TNF-α, and the transcription factor NF-κB in PBMC with the presence of antibody-dependent enhancement (ADE). Further, FVP has better DENV-1 antiviral activities compared to QS. However, in comparison with QS, FVP showed lower anti-inflammatory activities. Further studies are needed to explore the antiviral and anti-inflammatory mechanism of FVP and QS in the DENV-infected models. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 150-162]https://www.bsmiab.org/jabet/?mno=214390antiviral dengueanti-inflammatoryfavipiravirquinine sulfate
spellingShingle Regita Aulia Rosalina
Beti Ernawati Dewi
Fithriyah Fithriyah
Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
Journal of Advanced Biotechnology and Experimental Therapeutics
antiviral dengue
anti-inflammatory
favipiravir
quinine sulfate
title Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
title_full Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
title_fullStr Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
title_full_unstemmed Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
title_short Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
title_sort antiviral and anti inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro
topic antiviral dengue
anti-inflammatory
favipiravir
quinine sulfate
url https://www.bsmiab.org/jabet/?mno=214390
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AT betiernawatidewi antiviralandantiinflammatoryactivitiesoffavipiravirandquininesulfateagainstdenguevirusserotype1invitro
AT fithriyahfithriyah antiviralandantiinflammatoryactivitiesoffavipiravirandquininesulfateagainstdenguevirusserotype1invitro