Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption

Macroporous resin is an efficient separation technology that plays a crucial role in the separation and purification of traditional Chinese medicine (TCM). However, the application of macroporous resins in TCM pharmaceuticals is hindered by serious fouling caused by the complex materials used in TCM...

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Main Authors: Ruihong Wang, Hongbo Liu, Zhishu Tang, Huaxu Zhu, Huan Liu, Ran Guo, Zhongxing Song, Hongbo Xu, Bo Li, Guolong Li, Yue Zhang
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/21/5153
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author Ruihong Wang
Hongbo Liu
Zhishu Tang
Huaxu Zhu
Huan Liu
Ran Guo
Zhongxing Song
Hongbo Xu
Bo Li
Guolong Li
Yue Zhang
author_facet Ruihong Wang
Hongbo Liu
Zhishu Tang
Huaxu Zhu
Huan Liu
Ran Guo
Zhongxing Song
Hongbo Xu
Bo Li
Guolong Li
Yue Zhang
author_sort Ruihong Wang
collection DOAJ
description Macroporous resin is an efficient separation technology that plays a crucial role in the separation and purification of traditional Chinese medicine (TCM). However, the application of macroporous resins in TCM pharmaceuticals is hindered by serious fouling caused by the complex materials used in TCM. This study examines the impact of ultrafiltration (UF) membrane technology on the macroporous resin adsorption behavior of TCM extracts. In this paper, <i>Bupleurum chinense</i> DC. (<i>B. chinense</i>) water extracts were included as an example to study the effect of UF pretreatment on the macroporous resin adsorption of total saponins. The study results indicated that the adsorption of total saponins constituents from the water extracts of <i>B. chinense</i> on the macroporous resin followed the pseudo-second-order kinetic model and the Langmuir model. The thermodynamic parameters of adsorption, including enthalpy changes and Gibbs free energies, were negative, while entropy changes were positive. These results demonstrated that the total saponin components form a monolayer adsorption layer by spontaneous thermal adsorption on the macroporous resin, and that the adsorption rate is not determined by the rate of intraparticle diffusion. Following treatment with a UF membrane with an average molecular weight cut-off of 50 kDa, the protein, starch, pectin, tannin, and other impurities in the water extracts of <i>B. chinense</i> were reduced, while the total saponin content was retained at 82.32%. The adsorption kinetic model of the saponin constituents on the macroporous resin remained unchanged and was consistent with both the second-order kinetic model and the Langmuir model; the adsorption rate of the second-order kinetic model increased by 1.3 times and in the Langmuir model at 25 °C, the adsorption performance improved by 1.16 times compared to the original extracts. This study revealed that UF technology as a pretreatment method can reduce the fouling of macroporous resin by TCM extracts and improve the adsorption performance of macroporous resin.
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spelling doaj-art-7a7a6a1ac0d845f0a6a75d7a352230fa2025-08-20T02:14:16ZengMDPI AGMolecules1420-30492024-10-012921515310.3390/molecules29215153Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin AdsorptionRuihong Wang0Hongbo Liu1Zhishu Tang2Huaxu Zhu3Huan Liu4Ran Guo5Zhongxing Song6Hongbo Xu7Bo Li8Guolong Li9Yue Zhang10State Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaState Key Laboratory of Research and Development for Characteristic Resources of Qin Medicine (Cultivation), Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaMacroporous resin is an efficient separation technology that plays a crucial role in the separation and purification of traditional Chinese medicine (TCM). However, the application of macroporous resins in TCM pharmaceuticals is hindered by serious fouling caused by the complex materials used in TCM. This study examines the impact of ultrafiltration (UF) membrane technology on the macroporous resin adsorption behavior of TCM extracts. In this paper, <i>Bupleurum chinense</i> DC. (<i>B. chinense</i>) water extracts were included as an example to study the effect of UF pretreatment on the macroporous resin adsorption of total saponins. The study results indicated that the adsorption of total saponins constituents from the water extracts of <i>B. chinense</i> on the macroporous resin followed the pseudo-second-order kinetic model and the Langmuir model. The thermodynamic parameters of adsorption, including enthalpy changes and Gibbs free energies, were negative, while entropy changes were positive. These results demonstrated that the total saponin components form a monolayer adsorption layer by spontaneous thermal adsorption on the macroporous resin, and that the adsorption rate is not determined by the rate of intraparticle diffusion. Following treatment with a UF membrane with an average molecular weight cut-off of 50 kDa, the protein, starch, pectin, tannin, and other impurities in the water extracts of <i>B. chinense</i> were reduced, while the total saponin content was retained at 82.32%. The adsorption kinetic model of the saponin constituents on the macroporous resin remained unchanged and was consistent with both the second-order kinetic model and the Langmuir model; the adsorption rate of the second-order kinetic model increased by 1.3 times and in the Langmuir model at 25 °C, the adsorption performance improved by 1.16 times compared to the original extracts. This study revealed that UF technology as a pretreatment method can reduce the fouling of macroporous resin by TCM extracts and improve the adsorption performance of macroporous resin.https://www.mdpi.com/1420-3049/29/21/5153traditional Chinese medicineultrafiltrationadsorptionmacroporous resin
spellingShingle Ruihong Wang
Hongbo Liu
Zhishu Tang
Huaxu Zhu
Huan Liu
Ran Guo
Zhongxing Song
Hongbo Xu
Bo Li
Guolong Li
Yue Zhang
Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
Molecules
traditional Chinese medicine
ultrafiltration
adsorption
macroporous resin
title Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
title_full Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
title_fullStr Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
title_full_unstemmed Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
title_short Effect of the Total Saponins of <i>Bupleurum chinense</i> DC. Water Extracts Following Ultrafiltration Pretreatment on Macroporous Resin Adsorption
title_sort effect of the total saponins of i bupleurum chinense i dc water extracts following ultrafiltration pretreatment on macroporous resin adsorption
topic traditional Chinese medicine
ultrafiltration
adsorption
macroporous resin
url https://www.mdpi.com/1420-3049/29/21/5153
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