Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease
Parkinson’s disease is a condition that affects the central nervous system in the brain and is brought on by a lack of dopamine. Uncontrollable tremors, uncoordinated movement, and stiffness characterize Parkinson’s disease. Until now, the medication for Parkinson’s disease is limited to relieve the...
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| Format: | Article |
| Language: | Indonesian |
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Poltekkes Kemenkes Yogyakarta
2023-05-01
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| Series: | Jurnal Teknologi Laboratorium |
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| Online Access: | https://teknolabjournal.com/index.php/Jtl/article/view/406 |
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| author | Marisca Evalina Gondokesumo Krisyanti Budipramana Putu Dea Angelita Putri Ni Putu Diah Nopitasari Martanty Aditya Liza Yudistira Yusan |
| author_facet | Marisca Evalina Gondokesumo Krisyanti Budipramana Putu Dea Angelita Putri Ni Putu Diah Nopitasari Martanty Aditya Liza Yudistira Yusan |
| author_sort | Marisca Evalina Gondokesumo |
| collection | DOAJ |
| description | Parkinson’s disease is a condition that affects the central nervous system in the brain and is brought on by a lack of dopamine. Uncontrollable tremors, uncoordinated movement, and stiffness characterize Parkinson’s disease. Until now, the medication for Parkinson’s disease is limited to relieve the symptoms and maintain the quality of life; thus, the progression of the disease can be delayed. In order to search for alternative therapy from herbs, Kluwih (Artocarpus camansi) has been used traditionally to relieve convulsants. This research aims to observe 96% ethanol extract of A. camansi leaves in dopamine and locomotor activity in adult male and female zebrafish (Dario rerio). The A. camansi extract concentration was 2.5; 5; 7.5; and 10 mg/ml for 28 days. Zebrafish locomotion was observed for 5 minutes on days 0; 7; 14; 21; and 28. ELISA measured the observations of dopamine after 28 days. The 96% ethanol extract of A. camansi leaves at 5 mg/ml can increase dopamine levels after induced with rotenone, but the dopamine level decreased at 7,5 and 10 mg/ml. The maximal concentration to increase locomotor activity is also at 5 mg/ml, along with dopamine concentration. Our findings revealed that 5 mg/ml of 96% ethanol extract of A. camansi leaves was the optimal dosage to stimulate dopamine release and enhance locomotor activity. |
| format | Article |
| id | doaj-art-7a72afa9f7014984b9cd1c00a34eae87 |
| institution | Kabale University |
| issn | 2338-5634 2580-0191 |
| language | Indonesian |
| publishDate | 2023-05-01 |
| publisher | Poltekkes Kemenkes Yogyakarta |
| record_format | Article |
| series | Jurnal Teknologi Laboratorium |
| spelling | doaj-art-7a72afa9f7014984b9cd1c00a34eae872025-01-03T15:40:13ZindPoltekkes Kemenkes YogyakartaJurnal Teknologi Laboratorium2338-56342580-01912023-05-01121142210.29238/teknolabjournal.v12i1.406358Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s diseaseMarisca Evalina Gondokesumo0Krisyanti Budipramana1Putu Dea Angelita Putri2Ni Putu Diah Nopitasari3Martanty Aditya4Liza Yudistira Yusan5University of SurabayaUniversity of SurabayaUniversity of SurabayaUniversity of SurabayaMa Chung UniversityHang Tuah UniversityParkinson’s disease is a condition that affects the central nervous system in the brain and is brought on by a lack of dopamine. Uncontrollable tremors, uncoordinated movement, and stiffness characterize Parkinson’s disease. Until now, the medication for Parkinson’s disease is limited to relieve the symptoms and maintain the quality of life; thus, the progression of the disease can be delayed. In order to search for alternative therapy from herbs, Kluwih (Artocarpus camansi) has been used traditionally to relieve convulsants. This research aims to observe 96% ethanol extract of A. camansi leaves in dopamine and locomotor activity in adult male and female zebrafish (Dario rerio). The A. camansi extract concentration was 2.5; 5; 7.5; and 10 mg/ml for 28 days. Zebrafish locomotion was observed for 5 minutes on days 0; 7; 14; 21; and 28. ELISA measured the observations of dopamine after 28 days. The 96% ethanol extract of A. camansi leaves at 5 mg/ml can increase dopamine levels after induced with rotenone, but the dopamine level decreased at 7,5 and 10 mg/ml. The maximal concentration to increase locomotor activity is also at 5 mg/ml, along with dopamine concentration. Our findings revealed that 5 mg/ml of 96% ethanol extract of A. camansi leaves was the optimal dosage to stimulate dopamine release and enhance locomotor activity.https://teknolabjournal.com/index.php/Jtl/article/view/406keluwihzebrafishparkinsonrotenonedopamine |
| spellingShingle | Marisca Evalina Gondokesumo Krisyanti Budipramana Putu Dea Angelita Putri Ni Putu Diah Nopitasari Martanty Aditya Liza Yudistira Yusan Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease Jurnal Teknologi Laboratorium keluwih zebrafish parkinson rotenone dopamine |
| title | Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease |
| title_full | Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease |
| title_fullStr | Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease |
| title_full_unstemmed | Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease |
| title_short | Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease |
| title_sort | keluwih artocarpus camansi extract effects in zebrafish models of parkinson s disease |
| topic | keluwih zebrafish parkinson rotenone dopamine |
| url | https://teknolabjournal.com/index.php/Jtl/article/view/406 |
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