Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography

Ascorbic acid and photosynthetic pigments content in leaves of the apple tree (Malus domestica Borkh.) subjected to drought and white clover (Trifolium repens L.) exposed to high ozone concentrations were measured by high performance liquid chromatography in order to detect oxidative stress. Measur...

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Main Authors: Helena ŠIRCELJ, Franc BATIČ
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 1998-09-01
Series:Acta Agriculturae Slovenica
Subjects:
Online Access:https://journals.uni-lj.si/aas/article/view/16014
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author Helena ŠIRCELJ
Franc BATIČ
author_facet Helena ŠIRCELJ
Franc BATIČ
author_sort Helena ŠIRCELJ
collection DOAJ
description Ascorbic acid and photosynthetic pigments content in leaves of the apple tree (Malus domestica Borkh.) subjected to drought and white clover (Trifolium repens L.) exposed to high ozone concentrations were measured by high performance liquid chromatography in order to detect oxidative stress. Measurements showed a significant difference between stressed and control plants. Water deficit increased ascorbic acid and zeaxanthin, and decreased total chlorophyll (a+b) and b-carotene in apple tree leaves. High concentrations of ozone increased ascorbic acid, total carotenoids, neoxanthin, zeaxanthin and b-carotene, and decreased violaxanthin and antheraxanthin in white clover leaves. Observed changes in ascorbic acid and photosynthetic pigment content in leaves of tested apple tree and white clover plants indicate that oxidative stress may have occured under stress conditions used in our experiments.
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spelling doaj-art-7a7082dce0084ba298e02c10b2f7a9c52025-08-20T02:59:11ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-09-0171110.14720/aas.1998.71.1.1601422407Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatographyHelena ŠIRCELJ0Franc BATIČ1University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia Ascorbic acid and photosynthetic pigments content in leaves of the apple tree (Malus domestica Borkh.) subjected to drought and white clover (Trifolium repens L.) exposed to high ozone concentrations were measured by high performance liquid chromatography in order to detect oxidative stress. Measurements showed a significant difference between stressed and control plants. Water deficit increased ascorbic acid and zeaxanthin, and decreased total chlorophyll (a+b) and b-carotene in apple tree leaves. High concentrations of ozone increased ascorbic acid, total carotenoids, neoxanthin, zeaxanthin and b-carotene, and decreased violaxanthin and antheraxanthin in white clover leaves. Observed changes in ascorbic acid and photosynthetic pigment content in leaves of tested apple tree and white clover plants indicate that oxidative stress may have occured under stress conditions used in our experiments. https://journals.uni-lj.si/aas/article/view/16014ascorbic acidphotosinthetic pigmentshigh performance liquid chromatographyoxidative stressozonedrought
spellingShingle Helena ŠIRCELJ
Franc BATIČ
Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
Acta Agriculturae Slovenica
ascorbic acid
photosinthetic pigments
high performance liquid chromatography
oxidative stress
ozone
drought
title Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
title_full Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
title_fullStr Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
title_full_unstemmed Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
title_short Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
title_sort detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography
topic ascorbic acid
photosinthetic pigments
high performance liquid chromatography
oxidative stress
ozone
drought
url https://journals.uni-lj.si/aas/article/view/16014
work_keys_str_mv AT helenasircelj detectingoxidativestressinleavesofappletreeandwhitecloverbyanalysisofascorbicacidandpigmentsusinghighperformanceliquidchromatography
AT francbatic detectingoxidativestressinleavesofappletreeandwhitecloverbyanalysisofascorbicacidandpigmentsusinghighperformanceliquidchromatography