Recent Advances in Amyloids Structural Studies and Thin Film Applications

Amyloids are protein-based biomaterials composed of fibrils with cross-β cores. Previously only associated with degenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, and diabetes, amyloids remain active and functional both in vivo and in vitro conditions, enabling a variety of app...

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Main Authors: Eugenia Pechkova, Stefano Fiordoro, Alberto Izzotti, Christian Riekel
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/30/14/2908
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author Eugenia Pechkova
Stefano Fiordoro
Alberto Izzotti
Christian Riekel
author_facet Eugenia Pechkova
Stefano Fiordoro
Alberto Izzotti
Christian Riekel
author_sort Eugenia Pechkova
collection DOAJ
description Amyloids are protein-based biomaterials composed of fibrils with cross-β cores. Previously only associated with degenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, and diabetes, amyloids remain active and functional both in vivo and in vitro conditions, enabling a variety of applications in medicine, nanotechnology, and biotechnology. This review aims to review the most advanced methods for amyloid fibril structural studies, with special attention on amyloid thin films. Selected advances of biomedical and biotechnological relevance will be outlined, and perspectives for future studies in the context of ongoing methodological progress will be discussed.
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spelling doaj-art-7a6ee605ee3647b7aefce5cdbb9907a72025-08-20T02:47:07ZengMDPI AGMolecules1420-30492025-07-013014290810.3390/molecules30142908Recent Advances in Amyloids Structural Studies and Thin Film ApplicationsEugenia Pechkova0Stefano Fiordoro1Alberto Izzotti2Christian Riekel3Laboratories of Biophysics and Nanotechnology, Department of Experimental Medicine (DIMES), University of Genova Medical School, Via A. Pastore, 3, 16132 Genova, ItalyDepartment of Health Science (DISSAL), University of Genova Medical School, 16132 Genova, ItalyDepartment of Experimental Medicine (DIMES), University of Genova Medical School, 16132 Genova, ItalyEuropean Synchrotron Radiation Facility, CS40220, F-38043 Grenoble Cedex 9, FranceAmyloids are protein-based biomaterials composed of fibrils with cross-β cores. Previously only associated with degenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, and diabetes, amyloids remain active and functional both in vivo and in vitro conditions, enabling a variety of applications in medicine, nanotechnology, and biotechnology. This review aims to review the most advanced methods for amyloid fibril structural studies, with special attention on amyloid thin films. Selected advances of biomedical and biotechnological relevance will be outlined, and perspectives for future studies in the context of ongoing methodological progress will be discussed.https://www.mdpi.com/1420-3049/30/14/2908amyloid fibrilsamyloid thin filmhigh brilliance X-ray diffractioncryo-electron microscopy
spellingShingle Eugenia Pechkova
Stefano Fiordoro
Alberto Izzotti
Christian Riekel
Recent Advances in Amyloids Structural Studies and Thin Film Applications
Molecules
amyloid fibrils
amyloid thin film
high brilliance X-ray diffraction
cryo-electron microscopy
title Recent Advances in Amyloids Structural Studies and Thin Film Applications
title_full Recent Advances in Amyloids Structural Studies and Thin Film Applications
title_fullStr Recent Advances in Amyloids Structural Studies and Thin Film Applications
title_full_unstemmed Recent Advances in Amyloids Structural Studies and Thin Film Applications
title_short Recent Advances in Amyloids Structural Studies and Thin Film Applications
title_sort recent advances in amyloids structural studies and thin film applications
topic amyloid fibrils
amyloid thin film
high brilliance X-ray diffraction
cryo-electron microscopy
url https://www.mdpi.com/1420-3049/30/14/2908
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AT albertoizzotti recentadvancesinamyloidsstructuralstudiesandthinfilmapplications
AT christianriekel recentadvancesinamyloidsstructuralstudiesandthinfilmapplications