Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis
ABSTRACT The presented research is focused on evaluating the influence of adding clinoptilolite (Z) on the multi‐component co‐digestion (AcoD) of sewage sludge (SS), citrus waste represented by orange peel (OP) and brewery spent grain (BSG). The experiments were conducted under mesophilic conditions...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-05-01
|
| Series: | GCB Bioenergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/gcbb.70029 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849311110584336384 |
|---|---|
| author | Aleksandra Szaja Agnieszka Montusiewicz Agnieszka Cydzik‐Kwiatkowska Sylwia Pasieczna‐Patkowska Magdalena Lebiocka |
| author_facet | Aleksandra Szaja Agnieszka Montusiewicz Agnieszka Cydzik‐Kwiatkowska Sylwia Pasieczna‐Patkowska Magdalena Lebiocka |
| author_sort | Aleksandra Szaja |
| collection | DOAJ |
| description | ABSTRACT The presented research is focused on evaluating the influence of adding clinoptilolite (Z) on the multi‐component co‐digestion (AcoD) of sewage sludge (SS), citrus waste represented by orange peel (OP) and brewery spent grain (BSG). The experiments were conducted under mesophilic conditions at 37°C in batch mode; 7 series with differing feedstock configurations were performed. The positive effect of Z was shown in each configuration, most strongly with SS:OP:BSG:Z. The production of methane was enhanced by 81%, in comparison to the co‐digestion of SS:OP:BSG without Z, reaching 362.4 mL CH4/g volatile solids (VS). The kinetics was also improved as the methane production rate increased to a similar extent, amounting to 17.3 mL CH4/g VS d. Moreover, it was observed that the lag phase was shortened and the contents of both limonene and phenols were reduced. The composition of the microbial communities was significantly altered by the addition of Z in AcoD of SS:OP and SS:OP:BSG. Therein, a unique microbiome structure with the highest percentage of unidentified sequences was found. The beneficial effect of Z was multifaceted and related to the reduction of the stress caused by the presence of limonene and phenols as well as the syntrophic relation between the microorganisms. |
| format | Article |
| id | doaj-art-7a6aac21cd62471c96f4ab04faef9559 |
| institution | Kabale University |
| issn | 1757-1693 1757-1707 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj-art-7a6aac21cd62471c96f4ab04faef95592025-08-20T03:53:32ZengWileyGCB Bioenergy1757-16931757-17072025-05-01175n/an/a10.1111/gcbb.70029Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome AnalysisAleksandra Szaja0Agnieszka Montusiewicz1Agnieszka Cydzik‐Kwiatkowska2Sylwia Pasieczna‐Patkowska3Magdalena Lebiocka4Faculty of Environmental Engineering Lublin University of Technology Lublin PolandFaculty of Environmental Engineering Lublin University of Technology Lublin PolandDepartment of Environmental Biotechnology, Faculty of Geoengineering University of Warmia and Mazury Olsztyn PolandDepartment of Chemical Technology, Faculty of Chemistry Maria Curie Skłodowska University Lublin PolandFaculty of Environmental Engineering Lublin University of Technology Lublin PolandABSTRACT The presented research is focused on evaluating the influence of adding clinoptilolite (Z) on the multi‐component co‐digestion (AcoD) of sewage sludge (SS), citrus waste represented by orange peel (OP) and brewery spent grain (BSG). The experiments were conducted under mesophilic conditions at 37°C in batch mode; 7 series with differing feedstock configurations were performed. The positive effect of Z was shown in each configuration, most strongly with SS:OP:BSG:Z. The production of methane was enhanced by 81%, in comparison to the co‐digestion of SS:OP:BSG without Z, reaching 362.4 mL CH4/g volatile solids (VS). The kinetics was also improved as the methane production rate increased to a similar extent, amounting to 17.3 mL CH4/g VS d. Moreover, it was observed that the lag phase was shortened and the contents of both limonene and phenols were reduced. The composition of the microbial communities was significantly altered by the addition of Z in AcoD of SS:OP and SS:OP:BSG. Therein, a unique microbiome structure with the highest percentage of unidentified sequences was found. The beneficial effect of Z was multifaceted and related to the reduction of the stress caused by the presence of limonene and phenols as well as the syntrophic relation between the microorganisms.https://doi.org/10.1111/gcbb.70029brewery spent graincitrus wasteco‐digestionDNA analysissewage sludgezeolite |
| spellingShingle | Aleksandra Szaja Agnieszka Montusiewicz Agnieszka Cydzik‐Kwiatkowska Sylwia Pasieczna‐Patkowska Magdalena Lebiocka Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis GCB Bioenergy brewery spent grain citrus waste co‐digestion DNA analysis sewage sludge zeolite |
| title | Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis |
| title_full | Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis |
| title_fullStr | Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis |
| title_full_unstemmed | Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis |
| title_short | Enhancing Methane Production Through Co‐Digestion of Sewage Sludge, Citrus Waste and Brewery Spend Grain With Natural Zeolite: Mechanisms and Microbiome Analysis |
| title_sort | enhancing methane production through co digestion of sewage sludge citrus waste and brewery spend grain with natural zeolite mechanisms and microbiome analysis |
| topic | brewery spent grain citrus waste co‐digestion DNA analysis sewage sludge zeolite |
| url | https://doi.org/10.1111/gcbb.70029 |
| work_keys_str_mv | AT aleksandraszaja enhancingmethaneproductionthroughcodigestionofsewagesludgecitruswasteandbreweryspendgrainwithnaturalzeolitemechanismsandmicrobiomeanalysis AT agnieszkamontusiewicz enhancingmethaneproductionthroughcodigestionofsewagesludgecitruswasteandbreweryspendgrainwithnaturalzeolitemechanismsandmicrobiomeanalysis AT agnieszkacydzikkwiatkowska enhancingmethaneproductionthroughcodigestionofsewagesludgecitruswasteandbreweryspendgrainwithnaturalzeolitemechanismsandmicrobiomeanalysis AT sylwiapasiecznapatkowska enhancingmethaneproductionthroughcodigestionofsewagesludgecitruswasteandbreweryspendgrainwithnaturalzeolitemechanismsandmicrobiomeanalysis AT magdalenalebiocka enhancingmethaneproductionthroughcodigestionofsewagesludgecitruswasteandbreweryspendgrainwithnaturalzeolitemechanismsandmicrobiomeanalysis |