Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy
MDR bacteria are an emerging global threat to public health, and the role of dogs in the rise of antimicrobial resistance is under investigation. This study investigated the fecal shedding of extended-spectrum β-lactamase (ESBL)-, AmpC- and carbapenemase (CP)-producing <i>Escherichia coli</...
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2024-11-01
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| author | Alessia Facchin Gabriele Ratti Joel Filipe Martina Penati Alessia L. Gazzonis Greta Masiero Paola Dall’Ara Giovanni L. Alborali Stefania Lauzi |
| author_facet | Alessia Facchin Gabriele Ratti Joel Filipe Martina Penati Alessia L. Gazzonis Greta Masiero Paola Dall’Ara Giovanni L. Alborali Stefania Lauzi |
| author_sort | Alessia Facchin |
| collection | DOAJ |
| description | MDR bacteria are an emerging global threat to public health, and the role of dogs in the rise of antimicrobial resistance is under investigation. This study investigated the fecal shedding of extended-spectrum β-lactamase (ESBL)-, AmpC- and carbapenemase (CP)-producing <i>Escherichia coli</i> and associated risk factors in dogs admitted to the Veterinary Teaching Hospital of Lodi, University of Milan, or other veterinary clinics and kennels in Northen Italy. Feces collected in 2020–2022 were microbiologically and molecularly analyzed. ESBL-/AmpC-/CP-producing <i>E. coli</i> was detected in 14/100 (14%) dogs. Eleven (11%), five (5%) and one (1%) dogs carried ESBL-, AmpC- and CP-producing <i>E. coli</i> phenotypes, respectively, supported by the PCR detection of <i>bla</i><sub>CTX-M</sub> and/or <i>bla</i><sub>TEM</sub> in ESBL-producing <i>E. coli</i>; <i>bla</i><sub>CMY-2</sub> and the presence of putative low-level AmpC production in AmpC-producing <i>E. coli</i>; and <i>bla</i><sub>OXA-48</sub> in CP-producing <i>E. coli</i>. Different combinations of resistance genes and genetic features were observed. Multidrug resistance was observed in 13/14 (92.9%) <i>E. coli</i> isolates. Binary logistic regression analysis showed that ESBL-/AmpC-/CP-producing <i>E. coli</i> fecal shedding tended to be associated with antibiotic treatment <i>(p</i> = 0.058; OR = 3.87). The detection of ESBL-/AmpC-producing <i>E. coli</i>, along with the presence of a carbapenemase-resistant <i>E. coli</i> isolate from domestic dogs, although still limited, emphasizes the need for antimicrobial stewardship and specific surveillance programs, particularly for CP-producing bacteria in companion animals. |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-7a5d8bc93eac43c59fb12f9657aaab3c2025-08-20T01:55:40ZengMDPI AGAnimals2076-26152024-11-011423335910.3390/ani14233359Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from ItalyAlessia Facchin0Gabriele Ratti1Joel Filipe2Martina Penati3Alessia L. Gazzonis4Greta Masiero5Paola Dall’Ara6Giovanni L. Alborali7Stefania Lauzi8Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi 7/9, 25124 Brescia, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyMDR bacteria are an emerging global threat to public health, and the role of dogs in the rise of antimicrobial resistance is under investigation. This study investigated the fecal shedding of extended-spectrum β-lactamase (ESBL)-, AmpC- and carbapenemase (CP)-producing <i>Escherichia coli</i> and associated risk factors in dogs admitted to the Veterinary Teaching Hospital of Lodi, University of Milan, or other veterinary clinics and kennels in Northen Italy. Feces collected in 2020–2022 were microbiologically and molecularly analyzed. ESBL-/AmpC-/CP-producing <i>E. coli</i> was detected in 14/100 (14%) dogs. Eleven (11%), five (5%) and one (1%) dogs carried ESBL-, AmpC- and CP-producing <i>E. coli</i> phenotypes, respectively, supported by the PCR detection of <i>bla</i><sub>CTX-M</sub> and/or <i>bla</i><sub>TEM</sub> in ESBL-producing <i>E. coli</i>; <i>bla</i><sub>CMY-2</sub> and the presence of putative low-level AmpC production in AmpC-producing <i>E. coli</i>; and <i>bla</i><sub>OXA-48</sub> in CP-producing <i>E. coli</i>. Different combinations of resistance genes and genetic features were observed. Multidrug resistance was observed in 13/14 (92.9%) <i>E. coli</i> isolates. Binary logistic regression analysis showed that ESBL-/AmpC-/CP-producing <i>E. coli</i> fecal shedding tended to be associated with antibiotic treatment <i>(p</i> = 0.058; OR = 3.87). The detection of ESBL-/AmpC-producing <i>E. coli</i>, along with the presence of a carbapenemase-resistant <i>E. coli</i> isolate from domestic dogs, although still limited, emphasizes the need for antimicrobial stewardship and specific surveillance programs, particularly for CP-producing bacteria in companion animals.https://www.mdpi.com/2076-2615/14/23/3359canineantimicrobial resistanceESBLAmpCcarbapenemase |
| spellingShingle | Alessia Facchin Gabriele Ratti Joel Filipe Martina Penati Alessia L. Gazzonis Greta Masiero Paola Dall’Ara Giovanni L. Alborali Stefania Lauzi Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy Animals canine antimicrobial resistance ESBL AmpC carbapenemase |
| title | Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy |
| title_full | Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy |
| title_fullStr | Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy |
| title_full_unstemmed | Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy |
| title_short | Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-Producing <i>Escherichia coli</i> in Dogs from Italy |
| title_sort | fecal carriage and risk factors associated with extended spectrum β lactamase ampc carbapenemase producing i escherichia coli i in dogs from italy |
| topic | canine antimicrobial resistance ESBL AmpC carbapenemase |
| url | https://www.mdpi.com/2076-2615/14/23/3359 |
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