Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis
Abstract Long intergenic non-coding RNA 00,511 (LINC00511) is considered an oncogene for various cancers. However, the association between LINC00511 single nucleotide polymorphisms (SNPs) and colorectal cancer (CRC) remains unclear. Our study aims to study whether LINC00511 SNPs could predict CRC su...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-10938-7 |
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| author | Eman F. Sanad Ahmad A. Hady Mohamed Ali Shorouk Eldash Nermeen H. Elmorshedy Farah Ayman Hams M. Khattab Amr Maher Hadeel Ashree Mahitab Abdelhady Mazen Mohamed Ahmed Adel Sajed Khalil Omar Alyan Ahmed Samir Alhassan A. Bakr Nadia M. Hamdy |
| author_facet | Eman F. Sanad Ahmad A. Hady Mohamed Ali Shorouk Eldash Nermeen H. Elmorshedy Farah Ayman Hams M. Khattab Amr Maher Hadeel Ashree Mahitab Abdelhady Mazen Mohamed Ahmed Adel Sajed Khalil Omar Alyan Ahmed Samir Alhassan A. Bakr Nadia M. Hamdy |
| author_sort | Eman F. Sanad |
| collection | DOAJ |
| description | Abstract Long intergenic non-coding RNA 00,511 (LINC00511) is considered an oncogene for various cancers. However, the association between LINC00511 single nucleotide polymorphisms (SNPs) and colorectal cancer (CRC) remains unclear. Our study aims to study whether LINC00511 SNPs could predict CRC susceptibility or prognosis, an important step-toward precision-health, based on an Egyptian CRC patient cohort. A total of 200 CRC patients and 200 cancer-free controls were genotyped for three LINC00511 SNPs − rs9906859, rs1558535, and rs17780195 using qRT-PCR. Studied SNPs were in strong linkage disequilibrium and moderately correlated in all groups. Genotype association concerning tumor stage, revealed rs1558535 AT and rs17780195 AG variants correlated significantly with CRC advanced stages (adjusted OR: 3.99 and 2.72), respectively. Logistic regression showed that rs1558535 and rs9906859 genotypes were associated with CRC. Haplotype analysis disclosed that ‘Trs155535Ars17780195Crs9906859’ mutant-wild-mutant haplotype has 1.5-fold increased CRC risk (OR: 1.46, 95% CI: 1.07–1.99). ‘Trs155535Ars17780195Trs9906859’ haplotype conferred fivefold lower CRC risk (OR: 0.20, 95% CI: 0.09–0.47). Epistasis analysis showed individuals heterozygote and homozygote or homozygote and heterozygote for rs1558535 and rs9906859 are at high risk for CRC. Both rs1558535 and rs17780195 were associated with late stages of CRC. A strong interaction was observed between rs1558535 and rs9906859 in predicting CRC risk. |
| format | Article |
| id | doaj-art-7a4ef39960dd4acb89a3e6da32022068 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
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| spelling | doaj-art-7a4ef39960dd4acb89a3e6da320220682025-08-20T03:45:48ZengNature PortfolioScientific Reports2045-23222025-08-0115111910.1038/s41598-025-10938-7Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosisEman F. Sanad0Ahmad A. Hady1Mohamed Ali2Shorouk Eldash3Nermeen H. Elmorshedy4Farah Ayman5Hams M. Khattab6Amr Maher7Hadeel Ashree8Mahitab Abdelhady9Mazen Mohamed10Ahmed Adel11Sajed Khalil12Omar Alyan13Ahmed Samir14Alhassan A. Bakr15Nadia M. Hamdy16Biochemistry Department, Faculty of Pharmacy, Ain Shams University Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Mansoura UniversityClinical Pharmacy Department, Faculty of Pharmacy, Ain Shams UniversityPharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE)Drug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityDrug Design Program Graduation Project Students, Faculty of Pharmacy, Ain Shams UniversityBiochemistry Department, Faculty of Pharmacy, Ain Shams UniversityAbstract Long intergenic non-coding RNA 00,511 (LINC00511) is considered an oncogene for various cancers. However, the association between LINC00511 single nucleotide polymorphisms (SNPs) and colorectal cancer (CRC) remains unclear. Our study aims to study whether LINC00511 SNPs could predict CRC susceptibility or prognosis, an important step-toward precision-health, based on an Egyptian CRC patient cohort. A total of 200 CRC patients and 200 cancer-free controls were genotyped for three LINC00511 SNPs − rs9906859, rs1558535, and rs17780195 using qRT-PCR. Studied SNPs were in strong linkage disequilibrium and moderately correlated in all groups. Genotype association concerning tumor stage, revealed rs1558535 AT and rs17780195 AG variants correlated significantly with CRC advanced stages (adjusted OR: 3.99 and 2.72), respectively. Logistic regression showed that rs1558535 and rs9906859 genotypes were associated with CRC. Haplotype analysis disclosed that ‘Trs155535Ars17780195Crs9906859’ mutant-wild-mutant haplotype has 1.5-fold increased CRC risk (OR: 1.46, 95% CI: 1.07–1.99). ‘Trs155535Ars17780195Trs9906859’ haplotype conferred fivefold lower CRC risk (OR: 0.20, 95% CI: 0.09–0.47). Epistasis analysis showed individuals heterozygote and homozygote or homozygote and heterozygote for rs1558535 and rs9906859 are at high risk for CRC. Both rs1558535 and rs17780195 were associated with late stages of CRC. A strong interaction was observed between rs1558535 and rs9906859 in predicting CRC risk.https://doi.org/10.1038/s41598-025-10938-7Long intergenic non-coding RNA 005 (LINC00511)Single nucleotide polymorphism (SNPs)Colorectal cancer (CRC) |
| spellingShingle | Eman F. Sanad Ahmad A. Hady Mohamed Ali Shorouk Eldash Nermeen H. Elmorshedy Farah Ayman Hams M. Khattab Amr Maher Hadeel Ashree Mahitab Abdelhady Mazen Mohamed Ahmed Adel Sajed Khalil Omar Alyan Ahmed Samir Alhassan A. Bakr Nadia M. Hamdy Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis Scientific Reports Long intergenic non-coding RNA 005 (LINC00511) Single nucleotide polymorphism (SNPs) Colorectal cancer (CRC) |
| title | Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| title_full | Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| title_fullStr | Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| title_full_unstemmed | Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| title_short | Long intergenic non-coding RNA 00511 (LINC00511) genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| title_sort | long intergenic non coding rna 00511 linc00511 genetic variations and haplotype implication for colorectal cancer susceptibility and prognosis |
| topic | Long intergenic non-coding RNA 005 (LINC00511) Single nucleotide polymorphism (SNPs) Colorectal cancer (CRC) |
| url | https://doi.org/10.1038/s41598-025-10938-7 |
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