Growth, digestive enzyme and antioxidant enzyme activities of tawny puffer (Takifugu flavidus) larvae
It is well documented that, due to the changing nutritional requirements and shifting from endogenous to exogenous feeding, fishes experience evolutionary adaptations in the morphogenesis of their digestive system during early developmental stages. This fact is also reflected in the ontogeny of dige...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2014-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.12.191 |
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| Summary: | It is well documented that, due to the changing nutritional requirements and shifting from endogenous to exogenous feeding, fishes experience evolutionary adaptations in the morphogenesis of their digestive system during early developmental stages. This fact is also reflected in the ontogeny of digestive enzyme patterns. To maintain health and prevent oxidation-induced lesions and mortalities, there must be effective antioxidant systems operating in fish larvae. The components of these systems involve antioxidant compounds and antioxidant enzymes. It is well known and generally acknowledged that many biological and physiological systems are poorly developed in fish larvae. Therefore, a comprehensive analysis of the ontogenic changes occurring during the early life stages of fish is essential for the design of adequate larval rearing and feeding strategies and also for the formulation of dry diets.The overall aims of this study were to understand the characterization of the growth, digestive enzyme and antioxidant enzyme activities in tawny puffer (Takifugu flavidus) during the early development in order to enhance the growth and quality of early life stages by avoiding nutrient and oxidation problems that may cause pathologies and diseases.Total length, body mass, main digestive enzymes and antioxidant enzymes of tawny puffer larvae from 2 to 30 day-old were determined. The relationship between total length or body mass and day-old could be fixed to the linear and exponential functions, respectively. The general correlation equation of total length and body mass was power function under artificial farming conditions, and the growth (in mass) in relation to total length was allometric. Lipase activity was not detected in tawny puffer larvae. The activities of trypsin and pepsin followed a complementary variation pattern. Trypsin activity showed a rapid decrease from 2 to 6 day-old, and reached to the minimum level (109.17 U/mg) at 6 day-old. Meanwhile, pepsin activity increased significantly (P<0.05) and reached to the maximum level (1.09 U/mg) at 6 day-old. Amylase activity reached to the maximum (about 0.30 U/mg) at 2 and 19 day-old respectively, followed by the minimum (0.11 U/mg) at 15 day-old. Alkaline phosphatase had minimum activity (82.01 U/g) at 2 day-old, followed by the maximum (249.55 U/g) at 15 day-old. The activities of superoxide dismutase (SOD) and catalase (CAT) showed a similar variation tendency. Both the maximum levels (SOD:30.99 U/mg, CAT:646.36 U/g) appeared at 6 day-old. The SOD activity increased gradually from 10 day-old, while the CAT activity showed a wavy change.In conclusion, kinds of digestive and antioxidant enzymes have existed in the body of tawny puffer larvae before the preparation of their food intake, and the activities are closely related to the developmental stage and also are affected by external environment and feeding habit in different degrees. |
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| ISSN: | 1008-9209 2097-5155 |