Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema

<b>Background:</b> Diabetic macular edema (DME) is the leading cause of vision impairment in diabetic patients, with intravitreal anti-vascular endothelial growth factor (anti-VEGF) injections being the first-line therapy. However, one-third of patients exhibit persistent DME despite tre...

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Main Authors: Ece Ergin, Ana Maria Dascalu, Daniela Stana, Laura Carina Tribus, Andreea Letitia Arsene, Marina Ionela Nedea, Dragos Serban, Claudiu Eduard Nistor, Corneliu Tudor, Dan Dumitrescu, Paul Lorin Stoica, Bogdan Mihai Cristea
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Language:English
Published: MDPI AG 2025-05-01
Series:Biomedicines
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Online Access:https://www.mdpi.com/2227-9059/13/6/1308
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author Ece Ergin
Ana Maria Dascalu
Daniela Stana
Laura Carina Tribus
Andreea Letitia Arsene
Marina Ionela Nedea
Dragos Serban
Claudiu Eduard Nistor
Corneliu Tudor
Dan Dumitrescu
Paul Lorin Stoica
Bogdan Mihai Cristea
author_facet Ece Ergin
Ana Maria Dascalu
Daniela Stana
Laura Carina Tribus
Andreea Letitia Arsene
Marina Ionela Nedea
Dragos Serban
Claudiu Eduard Nistor
Corneliu Tudor
Dan Dumitrescu
Paul Lorin Stoica
Bogdan Mihai Cristea
author_sort Ece Ergin
collection DOAJ
description <b>Background:</b> Diabetic macular edema (DME) is the leading cause of vision impairment in diabetic patients, with intravitreal anti-vascular endothelial growth factor (anti-VEGF) injections being the first-line therapy. However, one-third of patients exhibit persistent DME despite treatment, suggesting additional pathogenic factors. This study aimed to evaluate the predictive value of complete blood count (CBC)-based inflammation indexes and optical coherence tomography (OCT) parameters in determining early anti-VEGF treatment effectiveness in DME. <b>Methods:</b> One hundred and four naïve patients with DME, treated with 0.05 mL of intravitreal aflibercept were retrospectively analyzed. Blood parameters analyzed included neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). Baseline OCT biomarkers included subretinal fluid (SRF), intraretinal cysts (IRC), hyperreflective retinal spots (HRS), and disorganization of retinal inner layers (DRIL). Treatment response was defined as a minimum 10% reduction in central macular thickness (CMT) at one month post-injection. <b>Results:</b> NLR, MLR, PLR, and SII were significantly higher in non-responders (<i>p</i> < 0.001), but their predictive value was fair, with an area under the ROC curve ranging between 0.704 (MLR) and 0.788 (SII). A multivariate model including SII, initial CMT, and the presence of IRC showed an excellent prediction value for early anatomical response (AUC ROC of 0.911). At the same time, lower PLR, DRIL, SRF, and the absence of HRF were correlated with early gain in BCVA. <b>Conclusions:</b> CBC-derived inflammation indices and OCT biomarkers have prognostic value in predicting early response to anti-VEGF therapy in DME in terms of functional and anatomical outcomes. These findings could help identify poor responders and guide personalized treatment strategies.
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spelling doaj-art-7a32c05991b04804968df2016e50b4982025-08-20T03:27:05ZengMDPI AGBiomedicines2227-90592025-05-01136130810.3390/biomedicines13061308Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular EdemaEce Ergin0Ana Maria Dascalu1Daniela Stana2Laura Carina Tribus3Andreea Letitia Arsene4Marina Ionela Nedea5Dragos Serban6Claudiu Eduard Nistor7Corneliu Tudor8Dan Dumitrescu9Paul Lorin Stoica10Bogdan Mihai Cristea11Doctoral School, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaDoctoral School, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaOphthalmology Department, Emergency University Hospital Bucharest, 050098 Bucharest, RomaniaFaculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaDoctoral School, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaDoctoral School, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, RomaniaFaculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, 020021 Bucharest, Romania<b>Background:</b> Diabetic macular edema (DME) is the leading cause of vision impairment in diabetic patients, with intravitreal anti-vascular endothelial growth factor (anti-VEGF) injections being the first-line therapy. However, one-third of patients exhibit persistent DME despite treatment, suggesting additional pathogenic factors. This study aimed to evaluate the predictive value of complete blood count (CBC)-based inflammation indexes and optical coherence tomography (OCT) parameters in determining early anti-VEGF treatment effectiveness in DME. <b>Methods:</b> One hundred and four naïve patients with DME, treated with 0.05 mL of intravitreal aflibercept were retrospectively analyzed. Blood parameters analyzed included neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). Baseline OCT biomarkers included subretinal fluid (SRF), intraretinal cysts (IRC), hyperreflective retinal spots (HRS), and disorganization of retinal inner layers (DRIL). Treatment response was defined as a minimum 10% reduction in central macular thickness (CMT) at one month post-injection. <b>Results:</b> NLR, MLR, PLR, and SII were significantly higher in non-responders (<i>p</i> < 0.001), but their predictive value was fair, with an area under the ROC curve ranging between 0.704 (MLR) and 0.788 (SII). A multivariate model including SII, initial CMT, and the presence of IRC showed an excellent prediction value for early anatomical response (AUC ROC of 0.911). At the same time, lower PLR, DRIL, SRF, and the absence of HRF were correlated with early gain in BCVA. <b>Conclusions:</b> CBC-derived inflammation indices and OCT biomarkers have prognostic value in predicting early response to anti-VEGF therapy in DME in terms of functional and anatomical outcomes. These findings could help identify poor responders and guide personalized treatment strategies.https://www.mdpi.com/2227-9059/13/6/1308diabetic macular edemaintravitreal anti-VEGFneutrophil-to-lymphocyte ratiosystemic inflammation indexplatelet-to-lymphocyte ratioOCT biomarkers
spellingShingle Ece Ergin
Ana Maria Dascalu
Daniela Stana
Laura Carina Tribus
Andreea Letitia Arsene
Marina Ionela Nedea
Dragos Serban
Claudiu Eduard Nistor
Corneliu Tudor
Dan Dumitrescu
Paul Lorin Stoica
Bogdan Mihai Cristea
Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
Biomedicines
diabetic macular edema
intravitreal anti-VEGF
neutrophil-to-lymphocyte ratio
systemic inflammation index
platelet-to-lymphocyte ratio
OCT biomarkers
title Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
title_full Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
title_fullStr Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
title_full_unstemmed Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
title_short Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Edema
title_sort predictive role of complete blood count derived inflammation indices and optical coherence tomography biomarkers for early response to intravitreal anti vegf in diabetic macular edema
topic diabetic macular edema
intravitreal anti-VEGF
neutrophil-to-lymphocyte ratio
systemic inflammation index
platelet-to-lymphocyte ratio
OCT biomarkers
url https://www.mdpi.com/2227-9059/13/6/1308
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