Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis
Galangin has activity modulating cell cycle arrest on luminal cancer cells and has high selectivity and low cytotoxicity for normal cells. This research intends to know galangin's prospective targets for promoting cell cycle arrest in luminal breast cancer via experimental in vitro, network ph...
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Bogor Agricultural University
2025-03-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/59628 |
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| author | Diyah Novi Sekarini Yohanes Surya Jati Nur Ayunie Zulkepli Dyaningtyas Dewi Pamungkas Putri |
| author_facet | Diyah Novi Sekarini Yohanes Surya Jati Nur Ayunie Zulkepli Dyaningtyas Dewi Pamungkas Putri |
| author_sort | Diyah Novi Sekarini |
| collection | DOAJ |
| description |
Galangin has activity modulating cell cycle arrest on luminal cancer cells and has high selectivity and low cytotoxicity for normal cells. This research intends to know galangin's prospective targets for promoting cell cycle arrest in luminal breast cancer via experimental in vitro, network pharmacology, and bioinformatics validation. In this research, MCF-7, a luminal model cell, was treated with galangin dose-dependent. Consequently, galangin exhibited a cytotoxic impact, with IC50 values of 117.86 μM. After that, SwissTargetPrediction, UALCAN, ShinyGO, and OncoLnc were used for bioinformatics validations, and Cytoscape software and the STRING website were used for computational analysis. Eight overlapping galangin target genes against luminal breast cancer were found. According to the analysis of the protein-protein interaction (PPI) network, eight hub genes-including CDK1, PLK1, TOP2A, ESR1, AURKB, NEK2, MMP9, and CA12-had the highest degree of freedom. Cell cycle regulation has been discovered to be tightly associated with overexpression of CDK1, PLK1, AURKB, and NEK2. By influencing the cell cycle, galangin inhibits the growth of luminal breast cancer, as determined by GO and KEGG enrichment analyses. In conclusion, by triggering cell cycle arrest, galangin may be used as a prospective chemotherapeutic treatment.
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| format | Article |
| id | doaj-art-7a300f1820a447538b6870eaaa540cdd |
| institution | DOAJ |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-7a300f1820a447538b6870eaaa540cdd2025-08-20T03:10:31ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942025-03-0132410.4308/hjb.32.4.969-979Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics AnalysisDiyah Novi Sekarini0Yohanes Surya Jati1Nur Ayunie Zulkepli2Dyaningtyas Dewi Pamungkas Putri3Department of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaCentre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, Selangor Malaysia. Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, Selangor MalaysiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. Pharmacology and Toxicology Laboratory, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. Laboratory of Advanced Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia Galangin has activity modulating cell cycle arrest on luminal cancer cells and has high selectivity and low cytotoxicity for normal cells. This research intends to know galangin's prospective targets for promoting cell cycle arrest in luminal breast cancer via experimental in vitro, network pharmacology, and bioinformatics validation. In this research, MCF-7, a luminal model cell, was treated with galangin dose-dependent. Consequently, galangin exhibited a cytotoxic impact, with IC50 values of 117.86 μM. After that, SwissTargetPrediction, UALCAN, ShinyGO, and OncoLnc were used for bioinformatics validations, and Cytoscape software and the STRING website were used for computational analysis. Eight overlapping galangin target genes against luminal breast cancer were found. According to the analysis of the protein-protein interaction (PPI) network, eight hub genes-including CDK1, PLK1, TOP2A, ESR1, AURKB, NEK2, MMP9, and CA12-had the highest degree of freedom. Cell cycle regulation has been discovered to be tightly associated with overexpression of CDK1, PLK1, AURKB, and NEK2. By influencing the cell cycle, galangin inhibits the growth of luminal breast cancer, as determined by GO and KEGG enrichment analyses. In conclusion, by triggering cell cycle arrest, galangin may be used as a prospective chemotherapeutic treatment. https://journal.ipb.ac.id/index.php/hayati/article/view/59628 |
| spellingShingle | Diyah Novi Sekarini Yohanes Surya Jati Nur Ayunie Zulkepli Dyaningtyas Dewi Pamungkas Putri Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis Hayati Journal of Biosciences |
| title | Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis |
| title_full | Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis |
| title_fullStr | Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis |
| title_full_unstemmed | Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis |
| title_short | Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis |
| title_sort | cell cycle target protein induced by galangin treatment in luminal cells confirmed by bioinformatics analysis |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/59628 |
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