Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China
The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is...
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KeAi Communications Co., Ltd.
2018-12-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918619719 |
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| author | Zhong-ling TIAN Munawar Maria Eda Marie Barsalote Pablo Castillo Jing-wu ZHENG |
| author_facet | Zhong-ling TIAN Munawar Maria Eda Marie Barsalote Pablo Castillo Jing-wu ZHENG |
| author_sort | Zhong-ling TIAN |
| collection | DOAJ |
| description | The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer (ITS) and cytochrome c oxidase subunit II (coxII)-16S rRNA genes and scanning electron microscopy (SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxII suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China. |
| format | Article |
| id | doaj-art-7a2f5f0434984b88bdabedb1501bbf45 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-7a2f5f0434984b88bdabedb1501bbf452025-08-20T03:57:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-12-0117122724273310.1016/S2095-3119(18)61971-9Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, ChinaZhong-ling TIAN0Munawar Maria1Eda Marie Barsalote2Pablo Castillo3Jing-wu ZHENG4Laboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China; TIAN Zhong-lingLaboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. ChinaLaboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. ChinaInstitute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, Córdoba 14004, SpainLaboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China; Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, P.R. China; Correspondence ZHENG Jing-wu, Tel/Fax: +86-571-88982580The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer (ITS) and cytochrome c oxidase subunit II (coxII)-16S rRNA genes and scanning electron microscopy (SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxII suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China.http://www.sciencedirect.com/science/article/pii/S2095311918619719Meloidogyne graminicolamorphologymorphometricmolecularricerDNA |
| spellingShingle | Zhong-ling TIAN Munawar Maria Eda Marie Barsalote Pablo Castillo Jing-wu ZHENG Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China Journal of Integrative Agriculture Meloidogyne graminicola morphology morphometric molecular rice rDNA |
| title | Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China |
| title_full | Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China |
| title_fullStr | Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China |
| title_full_unstemmed | Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China |
| title_short | Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China |
| title_sort | morphological and molecular characterization of the rice root knot nematode meloidogyne graminicola golden and birchfeild 1965 occurring in zhejiang china |
| topic | Meloidogyne graminicola morphology morphometric molecular rice rDNA |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918619719 |
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