Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients

Abstract Mesencephalic astrocyte-derived neurotrophic factor (MANF), a novel endoplasmic reticulum stress-induced neurotrophic factor, exerts a protective role in both nervous and non-nervous systems. However, the relationship between serum MANF content and community-acquired pneumonia (CAP) patient...

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Main Authors: Wen-Nan Wang, Xin-Li Zhang, Jian-Feng Xie, Man-Yi Tao, Lei Zhang, Zhen-Yu Cheng, Lin Fu, Jin Yang, Jun Fei
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97841-3
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author Wen-Nan Wang
Xin-Li Zhang
Jian-Feng Xie
Man-Yi Tao
Lei Zhang
Zhen-Yu Cheng
Lin Fu
Jin Yang
Jun Fei
author_facet Wen-Nan Wang
Xin-Li Zhang
Jian-Feng Xie
Man-Yi Tao
Lei Zhang
Zhen-Yu Cheng
Lin Fu
Jin Yang
Jun Fei
author_sort Wen-Nan Wang
collection DOAJ
description Abstract Mesencephalic astrocyte-derived neurotrophic factor (MANF), a novel endoplasmic reticulum stress-induced neurotrophic factor, exerts a protective role in both nervous and non-nervous systems. However, the relationship between serum MANF content and community-acquired pneumonia (CAP) patients remains unclear. A total of 319 adult patients diagnosed with CAP were enrolled. Serum MANF level was quantified using enzyme-linked immunosorbent assay (ELISA). A prospective cohort study was conducted to investigate the association of serum MANF levels with severity and prognosis. Compared with healthy volunteers, serum MANF content was evidently upregulated in CAP patients. The level of serum MANF was dramatically increased in parallel with the scores of CURB-65, PSI, APACHE II, and SMART-COP in CAP cases. Additionally, multivariate linear and logistic regression analyses revealed that serum MANF level was positively associated with scoring criteria among CAP patients. Besides, serum MANF level on admission was significantly elevated in CAP patients who required mechanical ventilation, ICU admission, and died within 30 days. Moreover, the higher serum MANF level on admission evidently increased the risk of poor prognosis during hospitalization. There was a better predictive power of serum MANF for poorly prognostic outcomes in CAP patients. The expression of serum MANF exhibits the positive correlations with the severity and poor prognosis among CAP patients. Serum MANF may have considerable clinical value and be regarded as a diagnostic and prognostic biomarker for CAP.
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spelling doaj-art-7a286575534341a5a0f69cea745e6a472025-08-20T03:10:14ZengNature PortfolioScientific Reports2045-23222025-04-011511810.1038/s41598-025-97841-3Associations of serum MANF with severity and prognosis in community-acquired pneumonia patientsWen-Nan Wang0Xin-Li Zhang1Jian-Feng Xie2Man-Yi Tao3Lei Zhang4Zhen-Yu Cheng5Lin Fu6Jin Yang7Jun Fei8Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversityDepartment of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversitySecond Department of Thoracic Surgery, Anhui Chest HospitalFifth Department of Tuberculosis, Anhui Chest HospitalFifth Department of Tuberculosis, Anhui Chest HospitalDepartment of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversityDepartment of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversityDepartment of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversityDepartment of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical UniversityAbstract Mesencephalic astrocyte-derived neurotrophic factor (MANF), a novel endoplasmic reticulum stress-induced neurotrophic factor, exerts a protective role in both nervous and non-nervous systems. However, the relationship between serum MANF content and community-acquired pneumonia (CAP) patients remains unclear. A total of 319 adult patients diagnosed with CAP were enrolled. Serum MANF level was quantified using enzyme-linked immunosorbent assay (ELISA). A prospective cohort study was conducted to investigate the association of serum MANF levels with severity and prognosis. Compared with healthy volunteers, serum MANF content was evidently upregulated in CAP patients. The level of serum MANF was dramatically increased in parallel with the scores of CURB-65, PSI, APACHE II, and SMART-COP in CAP cases. Additionally, multivariate linear and logistic regression analyses revealed that serum MANF level was positively associated with scoring criteria among CAP patients. Besides, serum MANF level on admission was significantly elevated in CAP patients who required mechanical ventilation, ICU admission, and died within 30 days. Moreover, the higher serum MANF level on admission evidently increased the risk of poor prognosis during hospitalization. There was a better predictive power of serum MANF for poorly prognostic outcomes in CAP patients. The expression of serum MANF exhibits the positive correlations with the severity and poor prognosis among CAP patients. Serum MANF may have considerable clinical value and be regarded as a diagnostic and prognostic biomarker for CAP.https://doi.org/10.1038/s41598-025-97841-3MANFCommunity-acquired pneumoniaSeverityPrognosisCohort study
spellingShingle Wen-Nan Wang
Xin-Li Zhang
Jian-Feng Xie
Man-Yi Tao
Lei Zhang
Zhen-Yu Cheng
Lin Fu
Jin Yang
Jun Fei
Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
Scientific Reports
MANF
Community-acquired pneumonia
Severity
Prognosis
Cohort study
title Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
title_full Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
title_fullStr Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
title_full_unstemmed Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
title_short Associations of serum MANF with severity and prognosis in community-acquired pneumonia patients
title_sort associations of serum manf with severity and prognosis in community acquired pneumonia patients
topic MANF
Community-acquired pneumonia
Severity
Prognosis
Cohort study
url https://doi.org/10.1038/s41598-025-97841-3
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