Isolation and purification of different high-purity cell populations from pig muscle tissue
Cultured meat technology is an emerging approach to meat production that generates edible meat tissue by cultivating animal-derived stem cells. Muscle stem cells (MuSCs) are essential seed cells for cultured meat production. However, due to the complexity of muscle tissue, obtaining highly pure MuSC...
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Maximum Academic Press
2025-01-01
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| Series: | Food Materials Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/fmr-0025-0001 |
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| author | Zenan Hu Zheng Liu Renpeng Guo Shijie Ding Guanghong Zhou |
| author_facet | Zenan Hu Zheng Liu Renpeng Guo Shijie Ding Guanghong Zhou |
| author_sort | Zenan Hu |
| collection | DOAJ |
| description | Cultured meat technology is an emerging approach to meat production that generates edible meat tissue by cultivating animal-derived stem cells. Muscle stem cells (MuSCs) are essential seed cells for cultured meat production. However, due to the complexity of muscle tissue, obtaining highly pure MuSCs and maintaining their purity during passaging remains a significant challenge. Our research addressed the issue by reevaluating the cell sorting strategy for porcine MuSCs and other cell types. A new combination of markers—CD31, CD45, JAM1, ITGA5, and ITGA7—were introduced here, sorting the muscle mononuclear cells into three distinct groups. Immunofluorescence staining and RNA-sequencing indicated three distinct cell types—MuSCs, smooth muscle cells (SMCs), and fibro-adipogenic progenitors (FAPs)—each displayed high expression levels of their characteristic markers. Additionally, after successive passaging, MuSCs obtained through this refined approach exhibited higher cell purity and improved myogenic properties compared to previous methods. Overall, this study presents a method for simultaneously obtaining MuSCs, SMCs, and FAPs with high purity from porcine muscle tissue, providing a high-quality source of seed cells for cultured meat production. |
| format | Article |
| id | doaj-art-7a21345e765b487891297c33a74a4e85 |
| institution | OA Journals |
| issn | 2771-4683 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Food Materials Research |
| spelling | doaj-art-7a21345e765b487891297c33a74a4e852025-08-20T02:12:30ZengMaximum Academic PressFood Materials Research2771-46832025-01-015111110.48130/fmr-0025-0001fmr-0025-0001Isolation and purification of different high-purity cell populations from pig muscle tissueZenan Hu0Zheng Liu1Renpeng Guo2Shijie Ding3Guanghong Zhou4State Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR ChinaState Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR ChinaState Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR ChinaJoes Future Food Technology Co. Ltd., Nanjing 211225, Jiangsu, PR ChinaState Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR ChinaCultured meat technology is an emerging approach to meat production that generates edible meat tissue by cultivating animal-derived stem cells. Muscle stem cells (MuSCs) are essential seed cells for cultured meat production. However, due to the complexity of muscle tissue, obtaining highly pure MuSCs and maintaining their purity during passaging remains a significant challenge. Our research addressed the issue by reevaluating the cell sorting strategy for porcine MuSCs and other cell types. A new combination of markers—CD31, CD45, JAM1, ITGA5, and ITGA7—were introduced here, sorting the muscle mononuclear cells into three distinct groups. Immunofluorescence staining and RNA-sequencing indicated three distinct cell types—MuSCs, smooth muscle cells (SMCs), and fibro-adipogenic progenitors (FAPs)—each displayed high expression levels of their characteristic markers. Additionally, after successive passaging, MuSCs obtained through this refined approach exhibited higher cell purity and improved myogenic properties compared to previous methods. Overall, this study presents a method for simultaneously obtaining MuSCs, SMCs, and FAPs with high purity from porcine muscle tissue, providing a high-quality source of seed cells for cultured meat production.https://www.maxapress.com/article/doi/10.48130/fmr-0025-0001cultured meatfacsrna-seqmuscssmcsfaps |
| spellingShingle | Zenan Hu Zheng Liu Renpeng Guo Shijie Ding Guanghong Zhou Isolation and purification of different high-purity cell populations from pig muscle tissue Food Materials Research cultured meat facs rna-seq muscs smcs faps |
| title | Isolation and purification of different high-purity cell populations from pig muscle tissue |
| title_full | Isolation and purification of different high-purity cell populations from pig muscle tissue |
| title_fullStr | Isolation and purification of different high-purity cell populations from pig muscle tissue |
| title_full_unstemmed | Isolation and purification of different high-purity cell populations from pig muscle tissue |
| title_short | Isolation and purification of different high-purity cell populations from pig muscle tissue |
| title_sort | isolation and purification of different high purity cell populations from pig muscle tissue |
| topic | cultured meat facs rna-seq muscs smcs faps |
| url | https://www.maxapress.com/article/doi/10.48130/fmr-0025-0001 |
| work_keys_str_mv | AT zenanhu isolationandpurificationofdifferenthighpuritycellpopulationsfrompigmuscletissue AT zhengliu isolationandpurificationofdifferenthighpuritycellpopulationsfrompigmuscletissue AT renpengguo isolationandpurificationofdifferenthighpuritycellpopulationsfrompigmuscletissue AT shijieding isolationandpurificationofdifferenthighpuritycellpopulationsfrompigmuscletissue AT guanghongzhou isolationandpurificationofdifferenthighpuritycellpopulationsfrompigmuscletissue |