Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes

Abstract Background Recently developed dynamic whole-body PET/CT (D-WB PET/CT) protocols allow for measurements of potentially more precise metabolic parameters than the commonly used semiquantitative SUV. Most notable is the metabolic rate of FDG uptake (MRFDG), which reflects quantitative glucose...

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Main Authors: Jonathan M. Baier, Kristian L. Funck, Anna Dons-Jensen, Ole L. Munk, Lars P. Tolbod, Esben Laugesen, Per L. Poulsen, Lars C. Gormsen, André H. Dias
Format: Article
Language:English
Published: SpringerOpen 2025-05-01
Series:EJNMMI Research
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Online Access:https://doi.org/10.1186/s13550-025-01249-z
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author Jonathan M. Baier
Kristian L. Funck
Anna Dons-Jensen
Ole L. Munk
Lars P. Tolbod
Esben Laugesen
Per L. Poulsen
Lars C. Gormsen
André H. Dias
author_facet Jonathan M. Baier
Kristian L. Funck
Anna Dons-Jensen
Ole L. Munk
Lars P. Tolbod
Esben Laugesen
Per L. Poulsen
Lars C. Gormsen
André H. Dias
author_sort Jonathan M. Baier
collection DOAJ
description Abstract Background Recently developed dynamic whole-body PET/CT (D-WB PET/CT) protocols allow for measurements of potentially more precise metabolic parameters than the commonly used semiquantitative SUV. Most notable is the metabolic rate of FDG uptake (MRFDG), which reflects quantitative glucose uptake into tissues and organs. However, data on the reproducibility of MRFDG measurements are scarce, particularly in patients with perturbed glucose homeostasis such as type 2 diabetes. We therefore aimed to evaluate the test-retest repeatability of both MRFDG and SUV in these patients. Results Fifteen participants (mean age 71 ± 7 years; 2 females) with type 2 diabetes underwent a short 20-minute [18F]FDG D-WB PET/CT after 6 h fasting on two consecutive days. Both SUV and MRFDG images were reconstructed from D-WB PET/CT data obtained 60–80 min post-injection of [18F]FDG. MRFDG and SUV data were measured in organs and tissues, and repeatability was assessed with Bland-Altman analysis, intraclass correlation coefficients (ICC), repeatability coefficients (RPC) and coefficients of variation (wCV). There was high repeatability of both SUVmean and MRFDG−mean in all measured organs (ICC range: 0.65–0.95 for SUVmean and 0.66–0.94 for MRFDG−mean). SUVmean generally demonstrated higher reliability (ICC) and lower variability (%RPC and %wCV) when compared to MRFDG−mean. However, MRFDG test-retest variation was < 19% in most analysed tissues, demonstrating that MRFDG may be used as a precise marker of treatment response. Conclusion This study demonstrates that MRFDG calculated from D-WB PET/CT exhibit high repeatability, comparable to SUVs across most organs in patients with type 2 diabetes.
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spelling doaj-art-7a106ad4dafb4cc98208bdc86f6969a82025-08-20T01:51:36ZengSpringerOpenEJNMMI Research2191-219X2025-05-0115111110.1186/s13550-025-01249-zTest-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetesJonathan M. Baier0Kristian L. Funck1Anna Dons-Jensen2Ole L. Munk3Lars P. Tolbod4Esben Laugesen5Per L. Poulsen6Lars C. Gormsen7André H. Dias8Dept. of Clinical Medicine, Aarhus UniversitySteno Diabetes Center Aarhus, Aarhus University HospitalSteno Diabetes Center Aarhus, Aarhus University HospitalDept. of Clinical Medicine, Aarhus UniversityDept. of Clinical Medicine, Aarhus UniversitySteno Diabetes Center Aarhus, Aarhus University HospitalDept. of Clinical Medicine, Aarhus UniversityDept. of Clinical Medicine, Aarhus UniversityDepartment of Nuclear Medicine & PET-Centre, Aarhus University HospitalAbstract Background Recently developed dynamic whole-body PET/CT (D-WB PET/CT) protocols allow for measurements of potentially more precise metabolic parameters than the commonly used semiquantitative SUV. Most notable is the metabolic rate of FDG uptake (MRFDG), which reflects quantitative glucose uptake into tissues and organs. However, data on the reproducibility of MRFDG measurements are scarce, particularly in patients with perturbed glucose homeostasis such as type 2 diabetes. We therefore aimed to evaluate the test-retest repeatability of both MRFDG and SUV in these patients. Results Fifteen participants (mean age 71 ± 7 years; 2 females) with type 2 diabetes underwent a short 20-minute [18F]FDG D-WB PET/CT after 6 h fasting on two consecutive days. Both SUV and MRFDG images were reconstructed from D-WB PET/CT data obtained 60–80 min post-injection of [18F]FDG. MRFDG and SUV data were measured in organs and tissues, and repeatability was assessed with Bland-Altman analysis, intraclass correlation coefficients (ICC), repeatability coefficients (RPC) and coefficients of variation (wCV). There was high repeatability of both SUVmean and MRFDG−mean in all measured organs (ICC range: 0.65–0.95 for SUVmean and 0.66–0.94 for MRFDG−mean). SUVmean generally demonstrated higher reliability (ICC) and lower variability (%RPC and %wCV) when compared to MRFDG−mean. However, MRFDG test-retest variation was < 19% in most analysed tissues, demonstrating that MRFDG may be used as a precise marker of treatment response. Conclusion This study demonstrates that MRFDG calculated from D-WB PET/CT exhibit high repeatability, comparable to SUVs across most organs in patients with type 2 diabetes.https://doi.org/10.1186/s13550-025-01249-zTest-retestPET/CTDynamic whole-body PETParametric imagingFDGDiabetes
spellingShingle Jonathan M. Baier
Kristian L. Funck
Anna Dons-Jensen
Ole L. Munk
Lars P. Tolbod
Esben Laugesen
Per L. Poulsen
Lars C. Gormsen
André H. Dias
Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
EJNMMI Research
Test-retest
PET/CT
Dynamic whole-body PET
Parametric imaging
FDG
Diabetes
title Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
title_full Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
title_fullStr Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
title_full_unstemmed Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
title_short Test-retest repeatability of quantitative organ and tissue uptake using 20-minute dynamic multiparametric whole-body [18F]FDG PET/CT in patients with type 2 diabetes
title_sort test retest repeatability of quantitative organ and tissue uptake using 20 minute dynamic multiparametric whole body 18f fdg pet ct in patients with type 2 diabetes
topic Test-retest
PET/CT
Dynamic whole-body PET
Parametric imaging
FDG
Diabetes
url https://doi.org/10.1186/s13550-025-01249-z
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