Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis
Loss of water through the immature skin can lead to hypothermia and dehydration in preterm infants. The water and glycerol channel aquaglyceroporin-3 (AQP3) is abundant in fetal epidermis and might influence epidermal water handling and transepidermal water flux around birth. To investigate the role...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2010/789729 |
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| author | Johan Agren Sergey Zelenin Lill-Britt Svensson Lene N. Nejsum Soren Nielsen Anita Aperia Gunnar Sedin |
| author_facet | Johan Agren Sergey Zelenin Lill-Britt Svensson Lene N. Nejsum Soren Nielsen Anita Aperia Gunnar Sedin |
| author_sort | Johan Agren |
| collection | DOAJ |
| description | Loss of water through the immature skin can lead to hypothermia and dehydration in preterm infants. The water and glycerol channel aquaglyceroporin-3 (AQP3) is abundant in fetal epidermis and might influence epidermal water handling and transepidermal water flux around birth. To investigate the role of AQP3 in immature skin, we measured in vivo transepidermal water transport and AQP3 expression in rat pups exposed to clinically relevant fluid homeostasis perturbations. Preterm (E18) rat pups were studied after antenatal corticosteroid exposure (ANS), and neonatal (P1) rat pups after an 18 h fast. Transepidermal water loss (TEWL) and skin hydration were determined, AQP3 mRNA was quantified by RT-PCR, and in-situ hybridization and immunocytochemistry were applied to map AQP3 expression. ANS resulted in an improved skin barrier (lower TEWL and skin hydration), while AQP3 mRNA and protein increased. Fasting led to loss of barrier integrity along with an increase in skin hydration. These alterations were not paralleled by any changes in AQP3. To conclude, antenatal corticosteroids and early postnatal fluid restriction produce differential effects on skin barrier function and epidermal AQP3 expression in the rat. In perinatal rats, AQP3 does not directly determine net water transport through the skin. |
| format | Article |
| id | doaj-art-7a078ff9f70a4360b3b2cafcb5acb32f |
| institution | Kabale University |
| issn | 1687-6105 1687-6113 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Dermatology Research and Practice |
| spelling | doaj-art-7a078ff9f70a4360b3b2cafcb5acb32f2025-08-20T03:35:48ZengWileyDermatology Research and Practice1687-61051687-61132010-01-01201010.1155/2010/789729789729Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat EpidermisJohan Agren0Sergey Zelenin1Lill-Britt Svensson2Lene N. Nejsum3Soren Nielsen4Anita Aperia5Gunnar Sedin6Department of Women's and Children's Health, Uppsala University, 751 85 Uppsala, SwedenDepartment of Woman and Child Health, Karolinska Institute, 171 77 Stockholm, SwedenDepartment of Woman and Child Health, Karolinska Institute, 171 77 Stockholm, SwedenThe Water and Salt Research Center, Aarhus University, 8000 Aarhus, DenmarkThe Water and Salt Research Center, Aarhus University, 8000 Aarhus, DenmarkDepartment of Woman and Child Health, Karolinska Institute, 171 77 Stockholm, SwedenDepartment of Women's and Children's Health, Uppsala University, 751 85 Uppsala, SwedenLoss of water through the immature skin can lead to hypothermia and dehydration in preterm infants. The water and glycerol channel aquaglyceroporin-3 (AQP3) is abundant in fetal epidermis and might influence epidermal water handling and transepidermal water flux around birth. To investigate the role of AQP3 in immature skin, we measured in vivo transepidermal water transport and AQP3 expression in rat pups exposed to clinically relevant fluid homeostasis perturbations. Preterm (E18) rat pups were studied after antenatal corticosteroid exposure (ANS), and neonatal (P1) rat pups after an 18 h fast. Transepidermal water loss (TEWL) and skin hydration were determined, AQP3 mRNA was quantified by RT-PCR, and in-situ hybridization and immunocytochemistry were applied to map AQP3 expression. ANS resulted in an improved skin barrier (lower TEWL and skin hydration), while AQP3 mRNA and protein increased. Fasting led to loss of barrier integrity along with an increase in skin hydration. These alterations were not paralleled by any changes in AQP3. To conclude, antenatal corticosteroids and early postnatal fluid restriction produce differential effects on skin barrier function and epidermal AQP3 expression in the rat. In perinatal rats, AQP3 does not directly determine net water transport through the skin.http://dx.doi.org/10.1155/2010/789729 |
| spellingShingle | Johan Agren Sergey Zelenin Lill-Britt Svensson Lene N. Nejsum Soren Nielsen Anita Aperia Gunnar Sedin Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis Dermatology Research and Practice |
| title | Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis |
| title_full | Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis |
| title_fullStr | Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis |
| title_full_unstemmed | Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis |
| title_short | Antenatal Corticosteroids and Postnatal Fluid Restriction Produce Differential Effects on AQP3 Expression, Water Handling, and Barrier Function in Perinatal Rat Epidermis |
| title_sort | antenatal corticosteroids and postnatal fluid restriction produce differential effects on aqp3 expression water handling and barrier function in perinatal rat epidermis |
| url | http://dx.doi.org/10.1155/2010/789729 |
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