Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates
Klebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. Extended spectrum β-lactamases (ESBLs) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. This study was undertaken to compare the virulence characterist...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2017/7279830 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849408345440518144 |
|---|---|
| author | Mustafa Muhammad Gharrah Areej Mostafa El-Mahdy Rasha Fathy Barwa |
| author_facet | Mustafa Muhammad Gharrah Areej Mostafa El-Mahdy Rasha Fathy Barwa |
| author_sort | Mustafa Muhammad Gharrah |
| collection | DOAJ |
| description | Klebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. Extended spectrum β-lactamases (ESBLs) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. This study was undertaken to compare the virulence characteristics and genetic relatedness between ESBL and non-ESBL producing K. pneumoniae. Methods. Antibiotic sensitivity test of all isolates was determined by disc diffusion assay. Phenotypic and genotypic detection of ESBL were done. Various virulence factors and some virulence factor-associated genes were screened. Random amplified polymorphic DNA (RAPD) was employed to investigate the genetic fingerprints of ESBL from non-ESBL producing K. pneumoniae. Results. 50% of isolates were ESBL producers. A significant association was observed between ESBL production and biofilm (strong and moderate), serum resistance, and iss gene. Moreover, significant association between non-ESBL producers and hypermucoviscosity was identified. Dendogram analysis of RAPD profile classified K. pneumoniae isolates into four clusters (a, b, c, and d). Seventy-six percent of ESBL producers belonged to cluster a. In conclusion, this study suggests a correlation between ESBL production and some virulence factors. Therefore, success of treatment depends mainly on increased clinicians awareness and enhanced testing by laboratories to reduce the spread of these isolates. |
| format | Article |
| id | doaj-art-7a03abd360644afea2a0e25538e84388 |
| institution | Kabale University |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-7a03abd360644afea2a0e25538e843882025-08-20T03:35:48ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982017-01-01201710.1155/2017/72798307279830Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing IsolatesMustafa Muhammad Gharrah0Areej Mostafa El-Mahdy1Rasha Fathy Barwa2Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptMicrobiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptMicrobiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptKlebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. Extended spectrum β-lactamases (ESBLs) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. This study was undertaken to compare the virulence characteristics and genetic relatedness between ESBL and non-ESBL producing K. pneumoniae. Methods. Antibiotic sensitivity test of all isolates was determined by disc diffusion assay. Phenotypic and genotypic detection of ESBL were done. Various virulence factors and some virulence factor-associated genes were screened. Random amplified polymorphic DNA (RAPD) was employed to investigate the genetic fingerprints of ESBL from non-ESBL producing K. pneumoniae. Results. 50% of isolates were ESBL producers. A significant association was observed between ESBL production and biofilm (strong and moderate), serum resistance, and iss gene. Moreover, significant association between non-ESBL producers and hypermucoviscosity was identified. Dendogram analysis of RAPD profile classified K. pneumoniae isolates into four clusters (a, b, c, and d). Seventy-six percent of ESBL producers belonged to cluster a. In conclusion, this study suggests a correlation between ESBL production and some virulence factors. Therefore, success of treatment depends mainly on increased clinicians awareness and enhanced testing by laboratories to reduce the spread of these isolates.http://dx.doi.org/10.1155/2017/7279830 |
| spellingShingle | Mustafa Muhammad Gharrah Areej Mostafa El-Mahdy Rasha Fathy Barwa Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates Interdisciplinary Perspectives on Infectious Diseases |
| title | Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates |
| title_full | Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates |
| title_fullStr | Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates |
| title_full_unstemmed | Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates |
| title_short | Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates |
| title_sort | association between virulence factors and extended spectrum beta lactamase producing klebsiella pneumoniae compared to nonproducing isolates |
| url | http://dx.doi.org/10.1155/2017/7279830 |
| work_keys_str_mv | AT mustafamuhammadgharrah associationbetweenvirulencefactorsandextendedspectrumbetalactamaseproducingklebsiellapneumoniaecomparedtononproducingisolates AT areejmostafaelmahdy associationbetweenvirulencefactorsandextendedspectrumbetalactamaseproducingklebsiellapneumoniaecomparedtononproducingisolates AT rashafathybarwa associationbetweenvirulencefactorsandextendedspectrumbetalactamaseproducingklebsiellapneumoniaecomparedtononproducingisolates |