Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland
Abstract Mycorrhiza interplays with the microbiome in adaptation to environmental fluctuation, yet how arbuscular mycorrhizal fungi (AMF) and the associated microbiome respond to nitrogen addition remains poorly understood. Here, we addressed this gap by conducting amplicon sequencing of AMF 18S rRN...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-08-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08681-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849761472559710208 |
|---|---|
| author | Jian-Xia Yang Yang Peng Jun-Jie Yang Yun-Hai Zhang Qiang Dong Qiu-Shi Li Xing-Guo Han Cheng Gao |
| author_facet | Jian-Xia Yang Yang Peng Jun-Jie Yang Yun-Hai Zhang Qiang Dong Qiu-Shi Li Xing-Guo Han Cheng Gao |
| author_sort | Jian-Xia Yang |
| collection | DOAJ |
| description | Abstract Mycorrhiza interplays with the microbiome in adaptation to environmental fluctuation, yet how arbuscular mycorrhizal fungi (AMF) and the associated microbiome respond to nitrogen addition remains poorly understood. Here, we addressed this gap by conducting amplicon sequencing of AMF 18S rRNA and bacterial 16S rRNA operons, along with shotgun metagenome sequencing, using soil samples collected from a semiarid grassland that has received nitrogen inputs for 11 years at different levels. We found that the nitrogen response of the AMF community was characterized by a negative association whereby increasing nitrogen addition leads to higher beta diversity and lower alpha diversity. Multiple co-inertia analyses revealed a coordinated response of the AMF community, bacterial community, and bacterial functions to nitrogen addition, which as a whole was strongly related to soil phosphorus availability. Besides, through network analysis of AMF with bacteria and bacterial functional genes, we found that nitrogen addition selected Actinobacteria and enriched functions of transporters, amino acid synthesis and metabolism, and replication repair, whereas there was no evidence for the enrichment of phosphorus mineralization functions. |
| format | Article |
| id | doaj-art-79faf925da564cf881aca336c4e4e050 |
| institution | DOAJ |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-79faf925da564cf881aca336c4e4e0502025-08-20T03:06:01ZengNature PortfolioCommunications Biology2399-36422025-08-018111110.1038/s42003-025-08681-wNitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grasslandJian-Xia Yang0Yang Peng1Jun-Jie Yang2Yun-Hai Zhang3Qiang Dong4Qiu-Shi Li5Xing-Guo Han6Cheng Gao7State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesSchool of Grassland Science, Beijing Forestry UniversityState Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of SciencesState Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesCollege of Life Sciences, Hebei UniversityState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesAbstract Mycorrhiza interplays with the microbiome in adaptation to environmental fluctuation, yet how arbuscular mycorrhizal fungi (AMF) and the associated microbiome respond to nitrogen addition remains poorly understood. Here, we addressed this gap by conducting amplicon sequencing of AMF 18S rRNA and bacterial 16S rRNA operons, along with shotgun metagenome sequencing, using soil samples collected from a semiarid grassland that has received nitrogen inputs for 11 years at different levels. We found that the nitrogen response of the AMF community was characterized by a negative association whereby increasing nitrogen addition leads to higher beta diversity and lower alpha diversity. Multiple co-inertia analyses revealed a coordinated response of the AMF community, bacterial community, and bacterial functions to nitrogen addition, which as a whole was strongly related to soil phosphorus availability. Besides, through network analysis of AMF with bacteria and bacterial functional genes, we found that nitrogen addition selected Actinobacteria and enriched functions of transporters, amino acid synthesis and metabolism, and replication repair, whereas there was no evidence for the enrichment of phosphorus mineralization functions.https://doi.org/10.1038/s42003-025-08681-w |
| spellingShingle | Jian-Xia Yang Yang Peng Jun-Jie Yang Yun-Hai Zhang Qiang Dong Qiu-Shi Li Xing-Guo Han Cheng Gao Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland Communications Biology |
| title | Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| title_full | Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| title_fullStr | Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| title_full_unstemmed | Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| title_short | Nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| title_sort | nitrogen addition alters arbuscular mycorrhizal fungi and soil bacteria networks without promoting phosphorus mineralization in a semiarid grassland |
| url | https://doi.org/10.1038/s42003-025-08681-w |
| work_keys_str_mv | AT jianxiayang nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT yangpeng nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT junjieyang nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT yunhaizhang nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT qiangdong nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT qiushili nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT xingguohan nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland AT chenggao nitrogenadditionaltersarbuscularmycorrhizalfungiandsoilbacterianetworkswithoutpromotingphosphorusmineralizationinasemiaridgrassland |