Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes
Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique. Methodology: T...
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| Format: | Article |
| Language: | English |
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University of Kufa, Faculty of Nursing
2014-08-01
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| Series: | Kufa Journal for Nursing Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2558 |
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| author | Jameel Jerri Yousif |
| author_facet | Jameel Jerri Yousif |
| author_sort | Jameel Jerri Yousif |
| collection | DOAJ |
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Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique.
Methodology: The study included, collect ( 104 ) stool samples of patients with diarrhea arrivals to Al-Sadar Medical City Hospital, Al- Zahra Maternity and Children Hospital, Al-Manathera hospital and Al-Sajjad Hospital.
Results: The results of the real-time PCR revealed, the presence of E. histolytica parasites in (25) stool samples out of the (104) samples, the rate (24%).
Conclusion: In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E. histolytica parasites in stool samples.
Recommendation: The study of gene expression of Actin and Amebapore-A genes, specific for E. histolytica parasite.
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| format | Article |
| id | doaj-art-79f960e2730e4ad0901d900ffd8aabeb |
| institution | OA Journals |
| issn | 2223-4055 2521-8638 |
| language | English |
| publishDate | 2014-08-01 |
| publisher | University of Kufa, Faculty of Nursing |
| record_format | Article |
| series | Kufa Journal for Nursing Sciences |
| spelling | doaj-art-79f960e2730e4ad0901d900ffd8aabeb2025-08-20T02:17:33ZengUniversity of Kufa, Faculty of NursingKufa Journal for Nursing Sciences2223-40552521-86382014-08-014210.36321/kjns.vi20142.2558Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A GenesJameel Jerri Yousif0University of Kufa Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique. Methodology: The study included, collect ( 104 ) stool samples of patients with diarrhea arrivals to Al-Sadar Medical City Hospital, Al- Zahra Maternity and Children Hospital, Al-Manathera hospital and Al-Sajjad Hospital. Results: The results of the real-time PCR revealed, the presence of E. histolytica parasites in (25) stool samples out of the (104) samples, the rate (24%). Conclusion: In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E. histolytica parasites in stool samples. Recommendation: The study of gene expression of Actin and Amebapore-A genes, specific for E. histolytica parasite. https://journal.uokufa.edu.iq/index.php/kjns/article/view/2558Real-Time PCREntamoeba histolyticaActinAmebapore-A |
| spellingShingle | Jameel Jerri Yousif Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes Kufa Journal for Nursing Sciences Real-Time PCR Entamoeba histolytica Actin Amebapore-A |
| title | Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes |
| title_full | Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes |
| title_fullStr | Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes |
| title_full_unstemmed | Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes |
| title_short | Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes |
| title_sort | molecular identification of entamoeba histolytica parasite by using actin and amebapore a genes |
| topic | Real-Time PCR Entamoeba histolytica Actin Amebapore-A |
| url | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2558 |
| work_keys_str_mv | AT jameeljerriyousif molecularidentificationofentamoebahistolyticaparasitebyusingactinandamebaporeagenes |