Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes
Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique. Methodology: T...
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| Format: | Article |
| Language: | English |
| Published: |
University of Kufa, Faculty of Nursing
2014-08-01
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| Series: | Kufa Journal for Nursing Sciences |
| Subjects: | |
| Online Access: | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2558 |
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| Summary: | Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique.
Methodology: The study included, collect ( 104 ) stool samples of patients with diarrhea arrivals to Al-Sadar Medical City Hospital, Al- Zahra Maternity and Children Hospital, Al-Manathera hospital and Al-Sajjad Hospital.
Results: The results of the real-time PCR revealed, the presence of E. histolytica parasites in (25) stool samples out of the (104) samples, the rate (24%).
Conclusion: In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E. histolytica parasites in stool samples.
Recommendation: The study of gene expression of Actin and Amebapore-A genes, specific for E. histolytica parasite.
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| ISSN: | 2223-4055 2521-8638 |