Scope of mean neutrophil volume as an indicator of neonatal sepsis

Background: Neonatal sepsis is one of the leading causes of mortality in developing countries because of its non-specific presentation. Blood culture is the gold standard for diagnosing sepsis, but culture results take 48 – 72 hours. Therefore, there is a need for an indicator that could be used as...

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Main Authors: Chaitra B E, Raja Parthiban, Shilpa G, Indrani Krishnappa, Impana Gowda
Format: Article
Language:English
Published: Association of Clinical Pathologists of Nepal 2022-09-01
Series:Journal of Pathology of Nepal
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Online Access:https://www.nepjol.info/index.php/JPN/article/view/43997
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author Chaitra B E
Raja Parthiban
Shilpa G
Indrani Krishnappa
Impana Gowda
author_facet Chaitra B E
Raja Parthiban
Shilpa G
Indrani Krishnappa
Impana Gowda
author_sort Chaitra B E
collection DOAJ
description Background: Neonatal sepsis is one of the leading causes of mortality in developing countries because of its non-specific presentation. Blood culture is the gold standard for diagnosing sepsis, but culture results take 48 – 72 hours. Therefore, there is a need for an indicator that could be used as a simple parameter to indicate the possibility of evolving sepsis to the clinician. The efficacy of mean neutrophil volume as an indicator must be evaluated. Materials and methods: The study aims to evaluate the utility of the mean neutrophil volume in the early diagnosis of neonatal sepsis. Following ethical clearance, the study involved analyzing mean neutrophil volume from peripheral smears of 50 newborns clinically diagnosed with neonatal sepsis. The control group included mean neutrophil volume findings from peripheral smears of normal newborns. Maternal or fetal factors like pregnancy-induced hypertension and birth asphyxia that could cause changes in neutrophils were excluded. The mean neutrophil value was calculated after establishing the mean diameter of 100 neutrophils per case using the National Institutes of Health Image J software. Results: The average mean neutrophil volume was found to be significantly increased in the sepsis group (181x104fl) than in the control group (739x103fl). A statistically significant difference (p= < 0.0001) in the mean neutrophil volume was observed. Conclusions: Mean neutrophil volume is a potential indicator to distinguish neonates with and without sepsis and help clinicians in the early diagnosis and management of sepsis.
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institution Kabale University
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publishDate 2022-09-01
publisher Association of Clinical Pathologists of Nepal
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spelling doaj-art-79d5b6fe641b473984cbdb94f886e97a2025-08-21T07:03:01ZengAssociation of Clinical Pathologists of NepalJournal of Pathology of Nepal2091-07972091-09082022-09-011221931193510.3126/jpn.v12i2.4399771062Scope of mean neutrophil volume as an indicator of neonatal sepsisChaitra B E0Raja Parthiban1Shilpa G2Indrani Krishnappa3https://orcid.org/0000-0002-5208-2621Impana Gowda4MVJ Medical College & Research Hospital, Banglalore, IndiaMVJ Medical College & Research Hospital, Banglalore, IndiaMVJ Medical College & Research Hospital, Banglalore, IndiaMVJ Medical College & Research Hospital, Banglalore, IndiaMVJ Medical College & Research Hospital, Banglalore, IndiaBackground: Neonatal sepsis is one of the leading causes of mortality in developing countries because of its non-specific presentation. Blood culture is the gold standard for diagnosing sepsis, but culture results take 48 – 72 hours. Therefore, there is a need for an indicator that could be used as a simple parameter to indicate the possibility of evolving sepsis to the clinician. The efficacy of mean neutrophil volume as an indicator must be evaluated. Materials and methods: The study aims to evaluate the utility of the mean neutrophil volume in the early diagnosis of neonatal sepsis. Following ethical clearance, the study involved analyzing mean neutrophil volume from peripheral smears of 50 newborns clinically diagnosed with neonatal sepsis. The control group included mean neutrophil volume findings from peripheral smears of normal newborns. Maternal or fetal factors like pregnancy-induced hypertension and birth asphyxia that could cause changes in neutrophils were excluded. The mean neutrophil value was calculated after establishing the mean diameter of 100 neutrophils per case using the National Institutes of Health Image J software. Results: The average mean neutrophil volume was found to be significantly increased in the sepsis group (181x104fl) than in the control group (739x103fl). A statistically significant difference (p= < 0.0001) in the mean neutrophil volume was observed. Conclusions: Mean neutrophil volume is a potential indicator to distinguish neonates with and without sepsis and help clinicians in the early diagnosis and management of sepsis.https://www.nepjol.info/index.php/JPN/article/view/43997inflammation; mean neutrophil volume; neonatal sepsis; sepsis;
spellingShingle Chaitra B E
Raja Parthiban
Shilpa G
Indrani Krishnappa
Impana Gowda
Scope of mean neutrophil volume as an indicator of neonatal sepsis
Journal of Pathology of Nepal
inflammation; mean neutrophil volume; neonatal sepsis; sepsis;
title Scope of mean neutrophil volume as an indicator of neonatal sepsis
title_full Scope of mean neutrophil volume as an indicator of neonatal sepsis
title_fullStr Scope of mean neutrophil volume as an indicator of neonatal sepsis
title_full_unstemmed Scope of mean neutrophil volume as an indicator of neonatal sepsis
title_short Scope of mean neutrophil volume as an indicator of neonatal sepsis
title_sort scope of mean neutrophil volume as an indicator of neonatal sepsis
topic inflammation; mean neutrophil volume; neonatal sepsis; sepsis;
url https://www.nepjol.info/index.php/JPN/article/view/43997
work_keys_str_mv AT chaitrabe scopeofmeanneutrophilvolumeasanindicatorofneonatalsepsis
AT rajaparthiban scopeofmeanneutrophilvolumeasanindicatorofneonatalsepsis
AT shilpag scopeofmeanneutrophilvolumeasanindicatorofneonatalsepsis
AT indranikrishnappa scopeofmeanneutrophilvolumeasanindicatorofneonatalsepsis
AT impanagowda scopeofmeanneutrophilvolumeasanindicatorofneonatalsepsis