Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes
Lactic acid bacteria are commonly present in various sources and possess significant probiotic properties. They can inhibit pathogenic bacteria and fungi simultaneously, making them promising candidates as bio-preservatives. This study investigated two potential probiotic strains: <i>Lactiplan...
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2025-02-01
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| author | Yuting Hou Yaoke Duan Guofang Wu Jianbo Zhang Xuan Luo Miao Zhang Huili Pang Yuxuan Hao Yanping Wang Yimin Cai Lei Wang Zhongfang Tan |
| author_facet | Yuting Hou Yaoke Duan Guofang Wu Jianbo Zhang Xuan Luo Miao Zhang Huili Pang Yuxuan Hao Yanping Wang Yimin Cai Lei Wang Zhongfang Tan |
| author_sort | Yuting Hou |
| collection | DOAJ |
| description | Lactic acid bacteria are commonly present in various sources and possess significant probiotic properties. They can inhibit pathogenic bacteria and fungi simultaneously, making them promising candidates as bio-preservatives. This study investigated two potential probiotic strains: <i>Lactiplantibacillus plantarum</i> LR5-2 (isolated from fermented meat products) and <i>Lacticaseibacillus rhamnosus</i> SQ63 (isolated from infant feces). The study evaluated their aggregation ability, anti-pathogenic activity, safety, and tolerance to gastrointestinal conditions, phenol, and bile salts. Additionally, their biological control potential against <i>Penicillium expansum</i> on fresh grapes was assessed. The results demonstrated that both strains exhibited high survival rates under extreme gastrointestinal conditions, enhanced Auto-aggregation, co-aggregation, and hydrophobicity. They displayed strong antioxidant activity and significant antibacterial effects against 11 pathogenic fungi and foodborne pathogens. Biosafety testing revealed that both strains are sensitive to most antibiotics, do not produce biogenic amines, and exhibit no hemolytic or DNase activity. In grapes, <i>L. plantarum</i> LR5-2 and <i>L. rhamnosus</i> SQ63 significantly reduced the incidence and disease index of <i>P. expansum</i> infection. In conclusion, the characterization analysis and bio-preservation experiments revealed that LR5-2 and SQ63 have strong potential as probiotics and bio-preservatives. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2025-02-01 |
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| series | Foods |
| spelling | doaj-art-79c96be04fd44dadb39f464c90d4e2d82025-08-20T02:12:25ZengMDPI AGFoods2304-81582025-02-0114349310.3390/foods14030493Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh GrapesYuting Hou0Yaoke Duan1Guofang Wu2Jianbo Zhang3Xuan Luo4Miao Zhang5Huili Pang6Yuxuan Hao7Yanping Wang8Yimin Cai9Lei Wang10Zhongfang Tan11Henan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaPlateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, ChinaPlateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, ChinaPlateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaPlateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaLactic acid bacteria are commonly present in various sources and possess significant probiotic properties. They can inhibit pathogenic bacteria and fungi simultaneously, making them promising candidates as bio-preservatives. This study investigated two potential probiotic strains: <i>Lactiplantibacillus plantarum</i> LR5-2 (isolated from fermented meat products) and <i>Lacticaseibacillus rhamnosus</i> SQ63 (isolated from infant feces). The study evaluated their aggregation ability, anti-pathogenic activity, safety, and tolerance to gastrointestinal conditions, phenol, and bile salts. Additionally, their biological control potential against <i>Penicillium expansum</i> on fresh grapes was assessed. The results demonstrated that both strains exhibited high survival rates under extreme gastrointestinal conditions, enhanced Auto-aggregation, co-aggregation, and hydrophobicity. They displayed strong antioxidant activity and significant antibacterial effects against 11 pathogenic fungi and foodborne pathogens. Biosafety testing revealed that both strains are sensitive to most antibiotics, do not produce biogenic amines, and exhibit no hemolytic or DNase activity. In grapes, <i>L. plantarum</i> LR5-2 and <i>L. rhamnosus</i> SQ63 significantly reduced the incidence and disease index of <i>P. expansum</i> infection. In conclusion, the characterization analysis and bio-preservation experiments revealed that LR5-2 and SQ63 have strong potential as probiotics and bio-preservatives.https://www.mdpi.com/2304-8158/14/3/493lactic acid bacteriaprobioticsantimicrobial propertiesbiological controlgrape |
| spellingShingle | Yuting Hou Yaoke Duan Guofang Wu Jianbo Zhang Xuan Luo Miao Zhang Huili Pang Yuxuan Hao Yanping Wang Yimin Cai Lei Wang Zhongfang Tan Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes Foods lactic acid bacteria probiotics antimicrobial properties biological control grape |
| title | Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes |
| title_full | Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes |
| title_fullStr | Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes |
| title_full_unstemmed | Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes |
| title_short | Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against <i>Penicillium expansum</i> on Fresh Grapes |
| title_sort | antibacterial activity probiotic potential and biocontrol efficacy of two lactic acid bacteria against i penicillium expansum i on fresh grapes |
| topic | lactic acid bacteria probiotics antimicrobial properties biological control grape |
| url | https://www.mdpi.com/2304-8158/14/3/493 |
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