Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis
Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03342rr02 |
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| _version_ | 1850152584802729984 |
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| author | John C. Fox Christopher A. Cave James W. Schumm |
| author_facet | John C. Fox Christopher A. Cave James W. Schumm |
| author_sort | John C. Fox |
| collection | DOAJ |
| description | Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15 000 forensic samples. |
| format | Article |
| id | doaj-art-79c2e956214b4b9fb894883f723eecfe |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-79c2e956214b4b9fb894883f723eecfe2025-08-20T02:25:56ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-02-0134231432210.2144/03342rr02Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic AnalysisJohn C. Fox0Christopher A. Cave1James W. Schumm21The Bode Technology Group, Springfield, VA, USA1The Bode Technology Group, Springfield, VA, USA1The Bode Technology Group, Springfield, VA, USAAccurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15 000 forensic samples.https://www.future-science.com/doi/10.2144/03342rr02 |
| spellingShingle | John C. Fox Christopher A. Cave James W. Schumm Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis BioTechniques |
| title | Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis |
| title_full | Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis |
| title_fullStr | Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis |
| title_full_unstemmed | Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis |
| title_short | Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis |
| title_sort | development characterization and validation of a sensitive primate specific quantification assay for forensic analysis |
| url | https://www.future-science.com/doi/10.2144/03342rr02 |
| work_keys_str_mv | AT johncfox developmentcharacterizationandvalidationofasensitiveprimatespecificquantificationassayforforensicanalysis AT christopheracave developmentcharacterizationandvalidationofasensitiveprimatespecificquantificationassayforforensicanalysis AT jameswschumm developmentcharacterizationandvalidationofasensitiveprimatespecificquantificationassayforforensicanalysis |