Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis
Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03342rr02 |
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| Summary: | Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15 000 forensic samples. |
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| ISSN: | 0736-6205 1940-9818 |