Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing
Achillea species, commonly referred to as yarrow, belong to the family Asteraceae and are typically perennial, outbreeding plants used in traditional medicine. The genus Achillea has undergone hybridization and polyploidization events, resulting in phylogenetically complex relationships. Considering...
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Taylor & Francis Group
2025-08-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23802359.2025.2535628 |
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| author | Hiroki Natsume Makoto Kusaba |
| author_facet | Hiroki Natsume Makoto Kusaba |
| author_sort | Hiroki Natsume |
| collection | DOAJ |
| description | Achillea species, commonly referred to as yarrow, belong to the family Asteraceae and are typically perennial, outbreeding plants used in traditional medicine. The genus Achillea has undergone hybridization and polyploidization events, resulting in phylogenetically complex relationships. Considering the limited scope of genomic studies on this genus, a genomic analysis of a diploid Achillea species is important for advancing our understanding of its evolutionary biology and genetic diversity. In this study, we assembled the complete chloroplast genome sequence of a diploid Achillea species (A. ptarmica) using nanopore long-read sequencing technology. The assembled chloroplast genome (149,252 bp), with a GC content of 38%, was revealed to include a large single-copy region (82,498 bp), a small single-copy region (18,458 bp), and two inverted repeat regions (24,148 bp each). The genome contains 89 protein-coding genes, 8 rRNAs, and 37 tRNAs. A phylogenetic analysis clustered A. ptarmica with other Achillea species (A. millefolium and A. wilsoniana), positioning them within the same clade as the genus Tanacetum. The accuracy of nanopore sequencing was validated by comparing the results for 10 chloroplast genes with the corresponding Sanger sequencing results. Our findings provide valuable genetic resources for further taxonomic, evolutionary, and phylogenetic studies of the genus Achillea. |
| format | Article |
| id | doaj-art-79b2841431b845daa7ead4e97c3aad94 |
| institution | DOAJ |
| issn | 2380-2359 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj-art-79b2841431b845daa7ead4e97c3aad942025-08-20T02:48:43ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592025-08-0110875375710.1080/23802359.2025.2535628Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencingHiroki Natsume0Makoto Kusaba1Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, JapanGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, JapanAchillea species, commonly referred to as yarrow, belong to the family Asteraceae and are typically perennial, outbreeding plants used in traditional medicine. The genus Achillea has undergone hybridization and polyploidization events, resulting in phylogenetically complex relationships. Considering the limited scope of genomic studies on this genus, a genomic analysis of a diploid Achillea species is important for advancing our understanding of its evolutionary biology and genetic diversity. In this study, we assembled the complete chloroplast genome sequence of a diploid Achillea species (A. ptarmica) using nanopore long-read sequencing technology. The assembled chloroplast genome (149,252 bp), with a GC content of 38%, was revealed to include a large single-copy region (82,498 bp), a small single-copy region (18,458 bp), and two inverted repeat regions (24,148 bp each). The genome contains 89 protein-coding genes, 8 rRNAs, and 37 tRNAs. A phylogenetic analysis clustered A. ptarmica with other Achillea species (A. millefolium and A. wilsoniana), positioning them within the same clade as the genus Tanacetum. The accuracy of nanopore sequencing was validated by comparing the results for 10 chloroplast genes with the corresponding Sanger sequencing results. Our findings provide valuable genetic resources for further taxonomic, evolutionary, and phylogenetic studies of the genus Achillea.https://www.tandfonline.com/doi/10.1080/23802359.2025.2535628Asteraceaenanoporephylogenetic analysis |
| spellingShingle | Hiroki Natsume Makoto Kusaba Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing Mitochondrial DNA. Part B. Resources Asteraceae nanopore phylogenetic analysis |
| title | Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing |
| title_full | Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing |
| title_fullStr | Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing |
| title_full_unstemmed | Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing |
| title_short | Complete sequence of the Achillea ptarmica chloroplast genome determined by long-read sequencing |
| title_sort | complete sequence of the achillea ptarmica chloroplast genome determined by long read sequencing |
| topic | Asteraceae nanopore phylogenetic analysis |
| url | https://www.tandfonline.com/doi/10.1080/23802359.2025.2535628 |
| work_keys_str_mv | AT hirokinatsume completesequenceoftheachilleaptarmicachloroplastgenomedeterminedbylongreadsequencing AT makotokusaba completesequenceoftheachilleaptarmicachloroplastgenomedeterminedbylongreadsequencing |