IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation
The interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulatio...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/747645 |
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| author | Pier Paolo Piccaluga Claudio Agostinelli Fabio Fuligni Simona Righi Claudio Tripodo Maria Carla Re Alberto Clò Anna Miserocchi Silvia Morini Marisa Gariglio Gian Gaetano Ferri Alberto Rinaldi-Ceroni Ottavio Piccin Marco De Andrea Stefano A. Pileri Santo Landolfo Davide Gibellini |
| author_facet | Pier Paolo Piccaluga Claudio Agostinelli Fabio Fuligni Simona Righi Claudio Tripodo Maria Carla Re Alberto Clò Anna Miserocchi Silvia Morini Marisa Gariglio Gian Gaetano Ferri Alberto Rinaldi-Ceroni Ottavio Piccin Marco De Andrea Stefano A. Pileri Santo Landolfo Davide Gibellini |
| author_sort | Pier Paolo Piccaluga |
| collection | DOAJ |
| description | The interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulation during maturation of B- and T-cells. We explored the role of IFI16 in normal B-cell subsets by analysing its expression and relationship with the major transcription factors involved in germinal center (GC) development and plasma-cell (PC) maturation. IFI16 mRNA was differentially expressed in B-cell subsets with significant decrease in IFI16 mRNA in GC and PCs with respect to naïve and memory subsets. IFI16 mRNA expression is inversely correlated with a few master regulators of B-cell differentiation such as BCL6, XBP1, POU2AF1, and BLIMP1. In contrast, IFI16 expression positively correlated with STAT3, REL, SPIB, RELA, RELB, IRF4, STAT5B, and STAT5A. ARACNE algorithm indicated a direct regulation of IFI16 by BCL6, STAT5B, and RELB, whereas the relationship between IFI16 and the other factors is modulated by intermediate factors. In addition, analysis of the CD40 signaling pathway showed that IFI16 gene expression directly correlated with NF-κB activation, indicating that IFI16 could be considered an upstream modulator of NF-κB in human B-cells. |
| format | Article |
| id | doaj-art-79b1eaf075e94380852eca3625ee107e |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-79b1eaf075e94380852eca3625ee107e2025-08-20T03:35:46ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/747645747645IFI16 Expression Is Related to Selected Transcription Factors during B-Cell DifferentiationPier Paolo Piccaluga0Claudio Agostinelli1Fabio Fuligni2Simona Righi3Claudio Tripodo4Maria Carla Re5Alberto Clò6Anna Miserocchi7Silvia Morini8Marisa Gariglio9Gian Gaetano Ferri10Alberto Rinaldi-Ceroni11Ottavio Piccin12Marco De Andrea13Stefano A. Pileri14Santo Landolfo15Davide Gibellini16Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Hematopathology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Hematopathology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Hematopathology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Hematopathology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Human Pathology, University of Palermo, 90127 Palermo, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Microbiology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Microbiology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Microbiology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Microbiology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Clinical and Experimental Medicine, Medical School of Novara, 28100 Novara, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Otolaryngology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Otolaryngology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Otolaryngology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Public Health and Microbiology, University of Turin, 10126 Turin, ItalyDepartment of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School Unit of Hematopathology, S. Orsola Malpighi Hospital, 40138 Bologna, ItalyDepartment of Public Health and Microbiology, University of Turin, 10126 Turin, ItalyDepartment of Pathology and Diagnostic, University of Verona, 35124 Verona, ItalyThe interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulation during maturation of B- and T-cells. We explored the role of IFI16 in normal B-cell subsets by analysing its expression and relationship with the major transcription factors involved in germinal center (GC) development and plasma-cell (PC) maturation. IFI16 mRNA was differentially expressed in B-cell subsets with significant decrease in IFI16 mRNA in GC and PCs with respect to naïve and memory subsets. IFI16 mRNA expression is inversely correlated with a few master regulators of B-cell differentiation such as BCL6, XBP1, POU2AF1, and BLIMP1. In contrast, IFI16 expression positively correlated with STAT3, REL, SPIB, RELA, RELB, IRF4, STAT5B, and STAT5A. ARACNE algorithm indicated a direct regulation of IFI16 by BCL6, STAT5B, and RELB, whereas the relationship between IFI16 and the other factors is modulated by intermediate factors. In addition, analysis of the CD40 signaling pathway showed that IFI16 gene expression directly correlated with NF-κB activation, indicating that IFI16 could be considered an upstream modulator of NF-κB in human B-cells.http://dx.doi.org/10.1155/2015/747645 |
| spellingShingle | Pier Paolo Piccaluga Claudio Agostinelli Fabio Fuligni Simona Righi Claudio Tripodo Maria Carla Re Alberto Clò Anna Miserocchi Silvia Morini Marisa Gariglio Gian Gaetano Ferri Alberto Rinaldi-Ceroni Ottavio Piccin Marco De Andrea Stefano A. Pileri Santo Landolfo Davide Gibellini IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation Journal of Immunology Research |
| title | IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation |
| title_full | IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation |
| title_fullStr | IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation |
| title_full_unstemmed | IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation |
| title_short | IFI16 Expression Is Related to Selected Transcription Factors during B-Cell Differentiation |
| title_sort | ifi16 expression is related to selected transcription factors during b cell differentiation |
| url | http://dx.doi.org/10.1155/2015/747645 |
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