Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine
Abstract Background This study aimed to develop ion-releasing and antibacterial resin-based dental sealants comprising 3 to 6 wt% monocalcium phosphate monohydrate (MCPM, M), 3 to 6 wt% bioactive glass (BAG, B), and 3 to 6 wt% polylysine (PLS, P). The physical properties, mechanical performance, cyt...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-025-05489-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594346177724416 |
|---|---|
| author | Phatpicha Lertwisitphon Yotsavee Worapasphaiboon Nichapa Champakanan Arnit Toneluck Parichart Naruphontjirakul Anne M. Young Rattapha Chinli Phoom Chairatana Supanan Sucharit Piyaphong Panpisut |
| author_facet | Phatpicha Lertwisitphon Yotsavee Worapasphaiboon Nichapa Champakanan Arnit Toneluck Parichart Naruphontjirakul Anne M. Young Rattapha Chinli Phoom Chairatana Supanan Sucharit Piyaphong Panpisut |
| author_sort | Phatpicha Lertwisitphon |
| collection | DOAJ |
| description | Abstract Background This study aimed to develop ion-releasing and antibacterial resin-based dental sealants comprising 3 to 6 wt% monocalcium phosphate monohydrate (MCPM, M), 3 to 6 wt% bioactive glass (BAG, B), and 3 to 6 wt% polylysine (PLS, P). The physical properties, mechanical performance, cytotoxicity, and inhibition of S. mutans biofilm by these materials were subsequently evaluated. Methods Five experimental dental sealants were formulated as follows: F1 (M6B6P6), F2 (M6B6P3), F3 (M3B3P6), F4 (M3B3P3), and F5 (M0B0P0, serving as the control). ClinproXT (CP, 3 M, Saint Paul, MN, USA) was used for commercial comparison. The degree of monomer conversion (DC) was determined using attenuated total reflectance-Fourier transform infrared spectroscopy (n = 5). The biaxial flexural strength (n = 6) and Vickers surface microhardness (n = 5) of the materials were evaluated after a 24-hour immersion in water. The element release over 4 weeks was measured using inductively coupled plasma-optical emission spectrometry (ICP-OES) (n = 3). The cell viability of mouse fibrosarcoma cells exposed to the extract was assessed via an MTT assay (n = 3). Additionally, the inhibition of S. mutans biofilm was tested (n = 3). Statistical analysis was conducted using one-way ANOVA and the Tukey HSD test. Results The lowest DC among experimental sealants was obtained from F1 (66 ± 4%), which was significantly higher than CP (54 ± 2%, p < 0.001). The lowest biaxial flexural strength was obtained from F3 (131 ± 47 MPa). This was comparable to that of CP (140 ± 58 MPa, p = 0.992). The lowest surface microhardness among experimental materials was detected with F2 (19 ± 2 Vickers hardness number), which was higher than that of CP (12 ± 1 Vickers hardness number, p = 0.003). Furthermore, high cell viability of > 90% after exposure to extracts from the experimental materials was detected, which was similar to that observed with CP. Additionally, the experimental materials exhibited higher Ca and P release compared to CP and showed a potential trend for reducing S. mutans biofilm formation. Increasing additive concentrations exhibited minimal effects on material properties, except for enhanced elemental release and a slight reduction in BFM with higher PLS content. Conclusion The experimental sealants provided sufficient physical and mechanical strength and maintained cell viability and bacterial inhibition with higher elemental release than the commercial product. |
| format | Article |
| id | doaj-art-79ab26f560a84931a859ab23dbf44712 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-79ab26f560a84931a859ab23dbf447122025-01-19T12:41:14ZengBMCBMC Oral Health1472-68312025-01-0125111310.1186/s12903-025-05489-2Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysinePhatpicha Lertwisitphon0Yotsavee Worapasphaiboon1Nichapa Champakanan2Arnit Toneluck3Parichart Naruphontjirakul4Anne M. Young5Rattapha Chinli6Phoom Chairatana7Supanan Sucharit8Piyaphong Panpisut9Mahidol Wittayanusorn SchoolMahidol Wittayanusorn SchoolMahidol Wittayanusorn SchoolFaculty of Dentistry, Thammasat UniversityBiological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology ThonburiDivision of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, Royal Free HospitalDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol UniversityMahidol Wittayanusorn SchoolFaculty of Dentistry, Thammasat UniversityAbstract Background This study aimed to develop ion-releasing and antibacterial resin-based dental sealants comprising 3 to 6 wt% monocalcium phosphate monohydrate (MCPM, M), 3 to 6 wt% bioactive glass (BAG, B), and 3 to 6 wt% polylysine (PLS, P). The physical properties, mechanical performance, cytotoxicity, and inhibition of S. mutans biofilm by these materials were subsequently evaluated. Methods Five experimental dental sealants were formulated as follows: F1 (M6B6P6), F2 (M6B6P3), F3 (M3B3P6), F4 (M3B3P3), and F5 (M0B0P0, serving as the control). ClinproXT (CP, 3 M, Saint Paul, MN, USA) was used for commercial comparison. The degree of monomer conversion (DC) was determined using attenuated total reflectance-Fourier transform infrared spectroscopy (n = 5). The biaxial flexural strength (n = 6) and Vickers surface microhardness (n = 5) of the materials were evaluated after a 24-hour immersion in water. The element release over 4 weeks was measured using inductively coupled plasma-optical emission spectrometry (ICP-OES) (n = 3). The cell viability of mouse fibrosarcoma cells exposed to the extract was assessed via an MTT assay (n = 3). Additionally, the inhibition of S. mutans biofilm was tested (n = 3). Statistical analysis was conducted using one-way ANOVA and the Tukey HSD test. Results The lowest DC among experimental sealants was obtained from F1 (66 ± 4%), which was significantly higher than CP (54 ± 2%, p < 0.001). The lowest biaxial flexural strength was obtained from F3 (131 ± 47 MPa). This was comparable to that of CP (140 ± 58 MPa, p = 0.992). The lowest surface microhardness among experimental materials was detected with F2 (19 ± 2 Vickers hardness number), which was higher than that of CP (12 ± 1 Vickers hardness number, p = 0.003). Furthermore, high cell viability of > 90% after exposure to extracts from the experimental materials was detected, which was similar to that observed with CP. Additionally, the experimental materials exhibited higher Ca and P release compared to CP and showed a potential trend for reducing S. mutans biofilm formation. Increasing additive concentrations exhibited minimal effects on material properties, except for enhanced elemental release and a slight reduction in BFM with higher PLS content. Conclusion The experimental sealants provided sufficient physical and mechanical strength and maintained cell viability and bacterial inhibition with higher elemental release than the commercial product.https://doi.org/10.1186/s12903-025-05489-2Resin sealantCalcium phosphatePolylysineBioactive glassDegree of monomer conversionBiaxial flexural strength |
| spellingShingle | Phatpicha Lertwisitphon Yotsavee Worapasphaiboon Nichapa Champakanan Arnit Toneluck Parichart Naruphontjirakul Anne M. Young Rattapha Chinli Phoom Chairatana Supanan Sucharit Piyaphong Panpisut Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine BMC Oral Health Resin sealant Calcium phosphate Polylysine Bioactive glass Degree of monomer conversion Biaxial flexural strength |
| title | Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine |
| title_full | Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine |
| title_fullStr | Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine |
| title_full_unstemmed | Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine |
| title_short | Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine |
| title_sort | enhancing elemental release and antibacterial properties of resin based dental sealants with calcium phosphate bioactive glass and polylysine |
| topic | Resin sealant Calcium phosphate Polylysine Bioactive glass Degree of monomer conversion Biaxial flexural strength |
| url | https://doi.org/10.1186/s12903-025-05489-2 |
| work_keys_str_mv | AT phatpichalertwisitphon enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT yotsaveeworapasphaiboon enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT nichapachampakanan enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT arnittoneluck enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT parichartnaruphontjirakul enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT annemyoung enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT rattaphachinli enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT phoomchairatana enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT supanansucharit enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine AT piyaphongpanpisut enhancingelementalreleaseandantibacterialpropertiesofresinbaseddentalsealantswithcalciumphosphatebioactiveglassandpolylysine |