Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles
In a previous study, we showed that administration of antigen-incorporated virus-like particles (VLPs) derived from simian virus 40 (SV40) induces production of cytotoxic T lymphocytes (CTL), as well as antibodies against the incorporated antigen(s), without the need for an adjuvant; however, it rem...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025001094 |
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| author | Takeharu Watanabe Masayuki Hayashi Masaaki Arai Sho Matsushita Hiroshi Handa Masaaki Kawano |
| author_facet | Takeharu Watanabe Masayuki Hayashi Masaaki Arai Sho Matsushita Hiroshi Handa Masaaki Kawano |
| author_sort | Takeharu Watanabe |
| collection | DOAJ |
| description | In a previous study, we showed that administration of antigen-incorporated virus-like particles (VLPs) derived from simian virus 40 (SV40) induces production of cytotoxic T lymphocytes (CTL), as well as antibodies against the incorporated antigen(s), without the need for an adjuvant; however, it remains unclear how immune cells recognize and respond to the SV40 capsid because SV40 VLPs did not upregulate expression of maturation markers on dendritic cells. In this study, administration of chicken ovalbumin (OVA) incorporated within SV40 VLPs induced interferon (IFN)-γ production in response to the OVA CTL epitope. IFN-γ production in response to the OVA CTL epitope was not inhibited in B cell-depleted mice, but it was inhibited in cluster of differentiation (CD)4+ T cell-depleted mice. Administration of SV40 VLPs upregulated expression of CD63/CD68/CD83/CD86/CD196, and induced secretion of C-C chemokine ligand (CCL)3 and CCL4, by B cells, as well as secretion of CCL4 by T cells and tumor necrosis factor-α by bone marrow-derived dendritic cells. |
| format | Article |
| id | doaj-art-79a7a22618904cf39105093c1714997f |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-79a7a22618904cf39105093c1714997f2025-02-02T05:28:02ZengElsevierHeliyon2405-84402025-01-01112e41729Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particlesTakeharu Watanabe0Masayuki Hayashi1Masaaki Arai2Sho Matsushita3Hiroshi Handa4Masaaki Kawano5Vaccine Sohyaku Group Modality Laboratories Sohyaku. Innovation Research Division Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, 227-0033, JapanVaccine Sohyaku Group Modality Laboratories Sohyaku. Innovation Research Division Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, 227-0033, JapanVaccine Sohyaku Group Modality Laboratories Sohyaku. Innovation Research Division Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, 227-0033, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan; Allergy Center, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, JapanDepartment of Molecular Pharmacology, Tokyo Medical University, Shinjuku, Tokyo, 160-8402, Japan; Center for Future Medical Research, Institute of Medical Science, Tokyo Medical University, Shinjuku, Tokyo, 160-8402, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan; Allergy Center, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan; Division of Analytical Science, Biomedical Research Center, Faculty of Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan; Corresponding author. Division of Analytical Science, Biomedical Research Center, Faculty of Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan.In a previous study, we showed that administration of antigen-incorporated virus-like particles (VLPs) derived from simian virus 40 (SV40) induces production of cytotoxic T lymphocytes (CTL), as well as antibodies against the incorporated antigen(s), without the need for an adjuvant; however, it remains unclear how immune cells recognize and respond to the SV40 capsid because SV40 VLPs did not upregulate expression of maturation markers on dendritic cells. In this study, administration of chicken ovalbumin (OVA) incorporated within SV40 VLPs induced interferon (IFN)-γ production in response to the OVA CTL epitope. IFN-γ production in response to the OVA CTL epitope was not inhibited in B cell-depleted mice, but it was inhibited in cluster of differentiation (CD)4+ T cell-depleted mice. Administration of SV40 VLPs upregulated expression of CD63/CD68/CD83/CD86/CD196, and induced secretion of C-C chemokine ligand (CCL)3 and CCL4, by B cells, as well as secretion of CCL4 by T cells and tumor necrosis factor-α by bone marrow-derived dendritic cells.http://www.sciencedirect.com/science/article/pii/S2405844025001094Simian virus 40Virus-like particlesImmune responsesCD86CCL3CCL4 |
| spellingShingle | Takeharu Watanabe Masayuki Hayashi Masaaki Arai Sho Matsushita Hiroshi Handa Masaaki Kawano Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles Heliyon Simian virus 40 Virus-like particles Immune responses CD86 CCL3 CCL4 |
| title | Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles |
| title_full | Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles |
| title_fullStr | Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles |
| title_full_unstemmed | Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles |
| title_short | Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles |
| title_sort | interferon γ production in response to the cytotoxic t lymphocyte epitope within an antigen incorporated in simian virus 40 virus like particles |
| topic | Simian virus 40 Virus-like particles Immune responses CD86 CCL3 CCL4 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025001094 |
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