Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus
IntroductionThe extensively used Lindera communis Hemsl. (Lauraceae) in traditional Chinese medicine has been specifically employed for wound healing and treating skin diseases in cattle and horses, suggesting its potential antibacterial properties. To explore the antibacterial activities of L. comm...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1528398/full |
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| author | Miaomiao Wang Miaomiao Wang Miaomiao Wang Xian Hu Xian Hu Xian Hu Liya Liu Liya Liu Liya Liu Liya Liu Yi Zhong Yi Zhong Yi Zhong Wanlin Li Wanlin Li Wanlin Li Qing Zhang Qing Zhang Qing Zhang Congli Xu Chunlin Long Chunlin Long Chunlin Long Chunlin Long Chunlin Long |
| author_facet | Miaomiao Wang Miaomiao Wang Miaomiao Wang Xian Hu Xian Hu Xian Hu Liya Liu Liya Liu Liya Liu Liya Liu Yi Zhong Yi Zhong Yi Zhong Wanlin Li Wanlin Li Wanlin Li Qing Zhang Qing Zhang Qing Zhang Congli Xu Chunlin Long Chunlin Long Chunlin Long Chunlin Long Chunlin Long |
| author_sort | Miaomiao Wang |
| collection | DOAJ |
| description | IntroductionThe extensively used Lindera communis Hemsl. (Lauraceae) in traditional Chinese medicine has been specifically employed for wound healing and treating skin diseases in cattle and horses, suggesting its potential antibacterial properties. To explore the antibacterial activities of L. communis plants, we investigated the chemicals, antibacterial activities and wound healing and of the n-hexane fraction of L. communis roots (LCH).MethodsOur study included detecting phytochemical constituents, determining minimum inhibitory concentration (MIC) for different extract fractions, analyzing growth curves, assessing membrane integrity, monitoring potential changes in the membrane using scanning electron microscopy, and evaluating wound healing in rat excisional wounds.ResultsBased on our findings, humulene-type sesquiterpenes, guaiane-type sesquiterpenes, and lauric acid were identified from the LCH, responsible for antibacterial and wound healing activities. The results are that LCH affected the growth of methicillin-resistant Staphylococcus aureus (MIC: 0.1 mg/mL) through morphological alterations and disrupting cell surface structures, causing membrane hyperpolarization and altering membrane integrity. This result was subsequently validated through SEM analysis and cytotoxicity against HaCaT cells (IC50 1.83 ± 0.21 mg/mL). LCH also has exhibited remarkable effectiveness in healing rat excisional wounds, reinforcing its traditional use as a wound-healing agent.DiscussionThe findings substantiate the scientific essence of traditional applications, while also exhibiting significant potential as a promising candidate for the development of innovative and readily accessible wound healing agents. |
| format | Article |
| id | doaj-art-79a6efbb81ab448db31d696f2b030956 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-79a6efbb81ab448db31d696f2b0309562025-02-11T09:13:30ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011610.3389/fphar.2025.15283981528398Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureusMiaomiao Wang0Miaomiao Wang1Miaomiao Wang2Xian Hu3Xian Hu4Xian Hu5Liya Liu6Liya Liu7Liya Liu8Liya Liu9Yi Zhong10Yi Zhong11Yi Zhong12Wanlin Li13Wanlin Li14Wanlin Li15Qing Zhang16Qing Zhang17Qing Zhang18Congli Xu19Chunlin Long20Chunlin Long21Chunlin Long22Chunlin Long23Chunlin Long24Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaCollege of Ethnology and Sociology, Minzu University of China, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaMass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaBaoshan Administration of Gaoligongshan National Nature Reserve, Baoshan, Yunnan, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaMass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaInstitute of National Security Studies, Minzu University of China, Beijing, ChinaIntroductionThe extensively used Lindera communis Hemsl. (Lauraceae) in traditional Chinese medicine has been specifically employed for wound healing and treating skin diseases in cattle and horses, suggesting its potential antibacterial properties. To explore the antibacterial activities of L. communis plants, we investigated the chemicals, antibacterial activities and wound healing and of the n-hexane fraction of L. communis roots (LCH).MethodsOur study included detecting phytochemical constituents, determining minimum inhibitory concentration (MIC) for different extract fractions, analyzing growth curves, assessing membrane integrity, monitoring potential changes in the membrane using scanning electron microscopy, and evaluating wound healing in rat excisional wounds.ResultsBased on our findings, humulene-type sesquiterpenes, guaiane-type sesquiterpenes, and lauric acid were identified from the LCH, responsible for antibacterial and wound healing activities. The results are that LCH affected the growth of methicillin-resistant Staphylococcus aureus (MIC: 0.1 mg/mL) through morphological alterations and disrupting cell surface structures, causing membrane hyperpolarization and altering membrane integrity. This result was subsequently validated through SEM analysis and cytotoxicity against HaCaT cells (IC50 1.83 ± 0.21 mg/mL). LCH also has exhibited remarkable effectiveness in healing rat excisional wounds, reinforcing its traditional use as a wound-healing agent.DiscussionThe findings substantiate the scientific essence of traditional applications, while also exhibiting significant potential as a promising candidate for the development of innovative and readily accessible wound healing agents.https://www.frontiersin.org/articles/10.3389/fphar.2025.1528398/fullLindera communisantibacterial activitymethicillin-resistant Staphylococcus aureushexane extractwound healing |
| spellingShingle | Miaomiao Wang Miaomiao Wang Miaomiao Wang Xian Hu Xian Hu Xian Hu Liya Liu Liya Liu Liya Liu Liya Liu Yi Zhong Yi Zhong Yi Zhong Wanlin Li Wanlin Li Wanlin Li Qing Zhang Qing Zhang Qing Zhang Congli Xu Chunlin Long Chunlin Long Chunlin Long Chunlin Long Chunlin Long Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus Frontiers in Pharmacology Lindera communis antibacterial activity methicillin-resistant Staphylococcus aureus hexane extract wound healing |
| title | Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus |
| title_full | Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus |
| title_fullStr | Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus |
| title_short | Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus |
| title_sort | hexane extract from lindera communis roots wound healing properties and membrane disruptive activities against methicillin resistant staphylococcus aureus |
| topic | Lindera communis antibacterial activity methicillin-resistant Staphylococcus aureus hexane extract wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1528398/full |
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