Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process
Low-temperature fracture is often observed during the low-temperature preservation of articular cartilage. However，the biomechanical damaging mechanism of cryopreservation is still unclear. During freezing from 0 ℃ to -60 ℃，Thermol Mechanical Analysis (TMA) was used to investigate the effects of 4 k...
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| Format: | Article |
| Language: | zho |
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Journal of Refrigeration Magazines Agency Co., Ltd.
2014-01-01
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| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2014.04.113 |
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| author | Yu Huaxing Li Daixi Xu Yi Liu Li Zhai Zhen Zhang Yan |
| author_facet | Yu Huaxing Li Daixi Xu Yi Liu Li Zhai Zhen Zhang Yan |
| author_sort | Yu Huaxing |
| collection | DOAJ |
| description | Low-temperature fracture is often observed during the low-temperature preservation of articular cartilage. However，the biomechanical damaging mechanism of cryopreservation is still unclear. During freezing from 0 ℃ to -60 ℃，Thermol Mechanical Analysis (TMA) was used to investigate the effects of 4 kinds of cryoprotecants with different concentrations. The results indicate that: (1) the cartilage produced a great thermal stress due to the extracellular thermal expansion during the cooling process；(2) when adding a higher concentration of ethanol and glycol，the faster the cooling rate，the greater thermal stress the articular cartilage will produce，and thus，slower cooling rate is more suitable for the cryopreservation of articular cartilage；(3) while adding 1, 2 - propylene and glycerin，there is an optimal cooling rate (30C/min) and it produces cartilage a minimal thermal stress. Moreover，cryoprotectants of higher methylation will contribute better protective effect to the thermal expansion among the 4 kinds of alcohols. |
| format | Article |
| id | doaj-art-79a05645ebf441218e00d04d34f0c428 |
| institution | OA Journals |
| issn | 0253-4339 |
| language | zho |
| publishDate | 2014-01-01 |
| publisher | Journal of Refrigeration Magazines Agency Co., Ltd. |
| record_format | Article |
| series | Zhileng xuebao |
| spelling | doaj-art-79a05645ebf441218e00d04d34f0c4282025-08-20T02:02:54ZzhoJournal of Refrigeration Magazines Agency Co., Ltd.Zhileng xuebao0253-43392014-01-013566515555Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing ProcessYu HuaxingLi DaixiXu YiLiu LiZhai ZhenZhang YanLow-temperature fracture is often observed during the low-temperature preservation of articular cartilage. However，the biomechanical damaging mechanism of cryopreservation is still unclear. During freezing from 0 ℃ to -60 ℃，Thermol Mechanical Analysis (TMA) was used to investigate the effects of 4 kinds of cryoprotecants with different concentrations. The results indicate that: (1) the cartilage produced a great thermal stress due to the extracellular thermal expansion during the cooling process；(2) when adding a higher concentration of ethanol and glycol，the faster the cooling rate，the greater thermal stress the articular cartilage will produce，and thus，slower cooling rate is more suitable for the cryopreservation of articular cartilage；(3) while adding 1, 2 - propylene and glycerin，there is an optimal cooling rate (30C/min) and it produces cartilage a minimal thermal stress. Moreover，cryoprotectants of higher methylation will contribute better protective effect to the thermal expansion among the 4 kinds of alcohols.http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2014.04.113cryopreservationthermal strainthermal mechanical analyzerarticular cartilageslow cooling rate |
| spellingShingle | Yu Huaxing Li Daixi Xu Yi Liu Li Zhai Zhen Zhang Yan Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process Zhileng xuebao cryopreservation thermal strain thermal mechanical analyzer articular cartilage slow cooling rate |
| title | Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process |
| title_full | Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process |
| title_fullStr | Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process |
| title_full_unstemmed | Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process |
| title_short | Effects of Cryoprotectant on Thermal Expansion of Articular Cartilage during Freezing Process |
| title_sort | effects of cryoprotectant on thermal expansion of articular cartilage during freezing process |
| topic | cryopreservation thermal strain thermal mechanical analyzer articular cartilage slow cooling rate |
| url | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2014.04.113 |
| work_keys_str_mv | AT yuhuaxing effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess AT lidaixi effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess AT xuyi effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess AT liuli effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess AT zhaizhen effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess AT zhangyan effectsofcryoprotectantonthermalexpansionofarticularcartilageduringfreezingprocess |