Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway
Objectives. To observe the effect of avβ3 single-stranded (ss) DNA on proliferation and migration of vascular smooth muscle cells (VSMCs) and its potential mechanism. Background. Percutaneous transluminal coronary angioplasty (PTCA) is currently the preferred method for the treatment of coronary hea...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2020/6869856 |
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| author | Hong-Bing Wu Zhi-Wei Wang Feng Shi Zong-Li Ren Luo-Cheng Li Xiao-Ping Hu Rui Hu Bo-Wen Li |
| author_facet | Hong-Bing Wu Zhi-Wei Wang Feng Shi Zong-Li Ren Luo-Cheng Li Xiao-Ping Hu Rui Hu Bo-Wen Li |
| author_sort | Hong-Bing Wu |
| collection | DOAJ |
| description | Objectives. To observe the effect of avβ3 single-stranded (ss) DNA on proliferation and migration of vascular smooth muscle cells (VSMCs) and its potential mechanism. Background. Percutaneous transluminal coronary angioplasty (PTCA) is currently the preferred method for the treatment of coronary heart disease. However, vascular restenosis still occurs after PTCA treatment, severely affecting the clinical efficacy of PTCA. Integrin avβ3, which is widely expressed on various cell surfaces, plays an important role in the proliferation and migration of VSMCs. Methods. In this experiment, we used systematic evolution of ligands by exponential enrichment (SELEX) to screen out avβ3 ssDNA, which has high affinity and specificity to the avβ3 protein. MTT, Transwell, and cell scratch assays were carried out to examine the effect of avβ3 ssDNA on the proliferation and migration of VSMCs. Flow cytometry was performed to detect apoptosis and cell cycle progression. The effect of avβ3 ssDNA on the Ras-phosphatidylinositol-4,5-bisphosphate 3-kinase/mitogen-activated protein kinase (PI3K/MAPK) signaling pathway was evaluated by quantitative reverse transcription polymerase chain reaction and western blot. Results. In the present study, we found that avβ3 ssDNA significantly decreased the expression of osteopontin, focal adhesion kinase, Ras, p-PI3K, and p-MAPK at both mRNA and protein levels (P<0.05). Avβ3 ssDNA also inhibited VSMC proliferation and migration while promoting apoptosis (P<0.05), as demonstrated by the upregulation of the proapoptotic proteins Bax and active caspase 3 (P<0.05). Conclusions. The findings suggest that avβ3 ssDNA inhibited the proliferation and migration of VSMCs by suppressing the activation of Ras-PI3K/MAPK signaling. |
| format | Article |
| id | doaj-art-7997f64993a64ac2a7b5352d1896ef46 |
| institution | Kabale University |
| issn | 1755-5914 1755-5922 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-7997f64993a64ac2a7b5352d1896ef462025-08-20T03:35:48ZengWileyCardiovascular Therapeutics1755-59141755-59222020-01-01202010.1155/2020/68698566869856Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK PathwayHong-Bing Wu0Zhi-Wei Wang1Feng Shi2Zong-Li Ren3Luo-Cheng Li4Xiao-Ping Hu5Rui Hu6Bo-Wen Li7Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaObjectives. To observe the effect of avβ3 single-stranded (ss) DNA on proliferation and migration of vascular smooth muscle cells (VSMCs) and its potential mechanism. Background. Percutaneous transluminal coronary angioplasty (PTCA) is currently the preferred method for the treatment of coronary heart disease. However, vascular restenosis still occurs after PTCA treatment, severely affecting the clinical efficacy of PTCA. Integrin avβ3, which is widely expressed on various cell surfaces, plays an important role in the proliferation and migration of VSMCs. Methods. In this experiment, we used systematic evolution of ligands by exponential enrichment (SELEX) to screen out avβ3 ssDNA, which has high affinity and specificity to the avβ3 protein. MTT, Transwell, and cell scratch assays were carried out to examine the effect of avβ3 ssDNA on the proliferation and migration of VSMCs. Flow cytometry was performed to detect apoptosis and cell cycle progression. The effect of avβ3 ssDNA on the Ras-phosphatidylinositol-4,5-bisphosphate 3-kinase/mitogen-activated protein kinase (PI3K/MAPK) signaling pathway was evaluated by quantitative reverse transcription polymerase chain reaction and western blot. Results. In the present study, we found that avβ3 ssDNA significantly decreased the expression of osteopontin, focal adhesion kinase, Ras, p-PI3K, and p-MAPK at both mRNA and protein levels (P<0.05). Avβ3 ssDNA also inhibited VSMC proliferation and migration while promoting apoptosis (P<0.05), as demonstrated by the upregulation of the proapoptotic proteins Bax and active caspase 3 (P<0.05). Conclusions. The findings suggest that avβ3 ssDNA inhibited the proliferation and migration of VSMCs by suppressing the activation of Ras-PI3K/MAPK signaling.http://dx.doi.org/10.1155/2020/6869856 |
| spellingShingle | Hong-Bing Wu Zhi-Wei Wang Feng Shi Zong-Li Ren Luo-Cheng Li Xiao-Ping Hu Rui Hu Bo-Wen Li Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway Cardiovascular Therapeutics |
| title | Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway |
| title_full | Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway |
| title_fullStr | Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway |
| title_full_unstemmed | Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway |
| title_short | Avβ3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway |
| title_sort | avβ3 single stranded dna aptamer attenuates vascular smooth muscle cell proliferation and migration via ras pi3k mapk pathway |
| url | http://dx.doi.org/10.1155/2020/6869856 |
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