Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria
Livestock are a source of zoonotic pathogens and bacteria harbouring antimicrobial resistance (AMR) that can pose a threat to public health. This study assessed the burden of antimicrobial resistance (AMR) and genomic diversity of <i>Salmonella</i> and <i>Escherichia coli</i>...
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MDPI AG
2025-05-01
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| author | Victoria Olusola Adetunji Alistair Davies Tom Chisnall Mwapu Dika Ndahi Idowu Oluwabunmi Fagbamila Eme Ekeng Ini Adebiyi Olutayo Israel Falodun Roderick M. Card |
| author_facet | Victoria Olusola Adetunji Alistair Davies Tom Chisnall Mwapu Dika Ndahi Idowu Oluwabunmi Fagbamila Eme Ekeng Ini Adebiyi Olutayo Israel Falodun Roderick M. Card |
| author_sort | Victoria Olusola Adetunji |
| collection | DOAJ |
| description | Livestock are a source of zoonotic pathogens and bacteria harbouring antimicrobial resistance (AMR) that can pose a threat to public health. This study assessed the burden of antimicrobial resistance (AMR) and genomic diversity of <i>Salmonella</i> and <i>Escherichia coli</i> at 25 poultry farms in Oyo State, Nigeria. The farm-level occurrence of <i>Salmonella</i> was 48%, with 12 serovars identified, including S. Kentucky Sequence Type 198. Notably, 70% of <i>Salmonella</i> isolates were resistant to fluoroquinolones, and multidrug resistance was observed in S. Kentucky and S. Derby. The study also found a 52% farm-level occurrence of extended-spectrum beta-lactamase-producing <i>E. coli</i>, with four bla<sub>CTX-M</sub> variants detected (bla<sub>CTX-M-15</sub>, bla<sub>CTX-M-27</sub>, bla<sub>CTX-M-55</sub>, and bla<sub>CTX-M-65</sub>). A genomic analysis revealed the dissemination of bacterial clones between farms, indicating potential routes of transmission. The high occurrence of AMR in both <i>Salmonella</i> and <i>E. coli</i> presents a potential public health risk, mainly through the consumption of contaminated poultry products. The study highlights the need for improved farm biosecurity and appropriate antimicrobial use to reduce the spread of resistant strains and combat AMR. These findings contribute to the Nigerian National Action Plan for AMR and underscore the importance of ongoing research and interventions in the poultry sector. |
| format | Article |
| id | doaj-art-798679c12d0b4ea7903b18bfb274e35b |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-798679c12d0b4ea7903b18bfb274e35b2025-08-20T03:16:19ZengMDPI AGMicroorganisms2076-26072025-05-01136117410.3390/microorganisms13061174Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, NigeriaVictoria Olusola Adetunji0Alistair Davies1Tom Chisnall2Mwapu Dika Ndahi3Idowu Oluwabunmi Fagbamila4Eme Ekeng5Ini Adebiyi6Olutayo Israel Falodun7Roderick M. Card8Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan 200005, NigeriaDepartment of Bacteriology, Animal and Plant Health Agency, Woodham Lane, Addlestone KT15 3NB, UKDepartment of Bacteriology, Animal and Plant Health Agency, Woodham Lane, Addlestone KT15 3NB, UKDepartment of Veterinary and Pest Control Services, Federal Ministry of Agriculture and Food Security, Area 11, Abuja 900103, NigeriaNational Veterinary Research Institute, Vom 930001, NigeriaNigeria Centre for Disease Control Laboratory, Abuja 900104, NigeriaUniversity College Hospital, Ibadan 200285, NigeriaDepartment of Microbiology, University of Ibadan, Ibadan 200005, NigeriaDepartment of Bacteriology, Animal and Plant Health Agency, Woodham Lane, Addlestone KT15 3NB, UKLivestock are a source of zoonotic pathogens and bacteria harbouring antimicrobial resistance (AMR) that can pose a threat to public health. This study assessed the burden of antimicrobial resistance (AMR) and genomic diversity of <i>Salmonella</i> and <i>Escherichia coli</i> at 25 poultry farms in Oyo State, Nigeria. The farm-level occurrence of <i>Salmonella</i> was 48%, with 12 serovars identified, including S. Kentucky Sequence Type 198. Notably, 70% of <i>Salmonella</i> isolates were resistant to fluoroquinolones, and multidrug resistance was observed in S. Kentucky and S. Derby. The study also found a 52% farm-level occurrence of extended-spectrum beta-lactamase-producing <i>E. coli</i>, with four bla<sub>CTX-M</sub> variants detected (bla<sub>CTX-M-15</sub>, bla<sub>CTX-M-27</sub>, bla<sub>CTX-M-55</sub>, and bla<sub>CTX-M-65</sub>). A genomic analysis revealed the dissemination of bacterial clones between farms, indicating potential routes of transmission. The high occurrence of AMR in both <i>Salmonella</i> and <i>E. coli</i> presents a potential public health risk, mainly through the consumption of contaminated poultry products. The study highlights the need for improved farm biosecurity and appropriate antimicrobial use to reduce the spread of resistant strains and combat AMR. These findings contribute to the Nigerian National Action Plan for AMR and underscore the importance of ongoing research and interventions in the poultry sector.https://www.mdpi.com/2076-2607/13/6/1174commercial poultry farms<i>Salmonella</i><i>Escherichia coli</i>antimicrobial resistanceNigeria |
| spellingShingle | Victoria Olusola Adetunji Alistair Davies Tom Chisnall Mwapu Dika Ndahi Idowu Oluwabunmi Fagbamila Eme Ekeng Ini Adebiyi Olutayo Israel Falodun Roderick M. Card Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria Microorganisms commercial poultry farms <i>Salmonella</i> <i>Escherichia coli</i> antimicrobial resistance Nigeria |
| title | Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria |
| title_full | Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria |
| title_fullStr | Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria |
| title_full_unstemmed | Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria |
| title_short | Genomic Diversity and Antibiotic Resistance of <i>Escherichia coli</i> and <i>Salmonella</i> from Poultry Farms in Oyo State, Nigeria |
| title_sort | genomic diversity and antibiotic resistance of i escherichia coli i and i salmonella i from poultry farms in oyo state nigeria |
| topic | commercial poultry farms <i>Salmonella</i> <i>Escherichia coli</i> antimicrobial resistance Nigeria |
| url | https://www.mdpi.com/2076-2607/13/6/1174 |
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