Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics
This study aimed to evaluate the therapeutic effect of <i>Terminalia chebula</i> (TC) on Tibetan yak-origin <i>Salmonella</i>-induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and...
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MDPI AG
2025-03-01
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| author | Dengyu Li Kaiqin Zhang Xiaofeng Xue Zhanchun Bai La Yang Jingjing Qi Sizhu Suolang |
| author_facet | Dengyu Li Kaiqin Zhang Xiaofeng Xue Zhanchun Bai La Yang Jingjing Qi Sizhu Suolang |
| author_sort | Dengyu Li |
| collection | DOAJ |
| description | This study aimed to evaluate the therapeutic effect of <i>Terminalia chebula</i> (TC) on Tibetan yak-origin <i>Salmonella</i>-induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and IL-10), and the oxidative stress markers malondialdehyde (MDA), superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), reduced glutathione (GSH-PX), and catalase (CAT) in the serum of mice were measured using ELISA kits. Using microbial diversity sequencing and non-targeted metabolomics detection techniques, the relevant mechanisms of TC treatment in a mouse <i>Salmonella</i> infection model were evaluated. The results showed the following: TC can effectively reduce the diarrhea rate; alleviate weight loss caused by <i>Salmonella</i> invasion; reduce the pro-inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α in serum; and increase the concentrations of the anti-inflammatory cytokines IL-4 and IL-10. TC can improve the body’s antioxidant levels to heal the damage caused by oxidative stress and lipid peroxidation. The histological section results show that TC can significantly improve gastric and intestinal tissue lesions and has no toxic effects on the liver and kidneys. 16S rRNA and ITS sequencing analysis suggests that <i>Lactobacillus</i>, <i>Enterorhabdus</i>, <i>Alistipes</i> (bacterial community), <i>Lodderomyces</i>, <i>Saccharomyces</i>, and <i>Penicillium</i> (fungal community) may be key functional microbial communities in TC. Non-targeted metabolomics also suggests that the antibacterial treatment of dysentery with chebulic acid may be related to regulation of the Ras signaling pathway, long-term potentiation, the MAPK signaling pathway, metabolic pathways, and gut microbiome composition. Conclusion: TC has clear clinical efficacy in treating bacterial diarrhea, presenting anti-inflammatory and antioxidant effects. Its roles in regulating the gut microbiome and metabolic pathways and products were determined as the main reason for its therapeutic effect in a mouse gastroenteritis model caused by <i>Salmonella</i> infection. |
| format | Article |
| id | doaj-art-797ddf02c6c149648e4da7b4c8ef7836 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-797ddf02c6c149648e4da7b4c8ef78362025-08-20T02:57:40ZengMDPI AGAnimals2076-26152025-03-0115575510.3390/ani15050755Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and MetabolomicsDengyu Li0Kaiqin Zhang1Xiaofeng Xue2Zhanchun Bai3La Yang4Jingjing Qi5Sizhu Suolang6College of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences China, Shanghai 200241, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, ChinaThis study aimed to evaluate the therapeutic effect of <i>Terminalia chebula</i> (TC) on Tibetan yak-origin <i>Salmonella</i>-induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and IL-10), and the oxidative stress markers malondialdehyde (MDA), superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), reduced glutathione (GSH-PX), and catalase (CAT) in the serum of mice were measured using ELISA kits. Using microbial diversity sequencing and non-targeted metabolomics detection techniques, the relevant mechanisms of TC treatment in a mouse <i>Salmonella</i> infection model were evaluated. The results showed the following: TC can effectively reduce the diarrhea rate; alleviate weight loss caused by <i>Salmonella</i> invasion; reduce the pro-inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α in serum; and increase the concentrations of the anti-inflammatory cytokines IL-4 and IL-10. TC can improve the body’s antioxidant levels to heal the damage caused by oxidative stress and lipid peroxidation. The histological section results show that TC can significantly improve gastric and intestinal tissue lesions and has no toxic effects on the liver and kidneys. 16S rRNA and ITS sequencing analysis suggests that <i>Lactobacillus</i>, <i>Enterorhabdus</i>, <i>Alistipes</i> (bacterial community), <i>Lodderomyces</i>, <i>Saccharomyces</i>, and <i>Penicillium</i> (fungal community) may be key functional microbial communities in TC. Non-targeted metabolomics also suggests that the antibacterial treatment of dysentery with chebulic acid may be related to regulation of the Ras signaling pathway, long-term potentiation, the MAPK signaling pathway, metabolic pathways, and gut microbiome composition. Conclusion: TC has clear clinical efficacy in treating bacterial diarrhea, presenting anti-inflammatory and antioxidant effects. Its roles in regulating the gut microbiome and metabolic pathways and products were determined as the main reason for its therapeutic effect in a mouse gastroenteritis model caused by <i>Salmonella</i> infection.https://www.mdpi.com/2076-2615/15/5/755<i>Terminalia chebula</i> (TC)gastroenteritisgut microbiomenon-targeted metabolism<i>Salmonella</i> |
| spellingShingle | Dengyu Li Kaiqin Zhang Xiaofeng Xue Zhanchun Bai La Yang Jingjing Qi Sizhu Suolang Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics Animals <i>Terminalia chebula</i> (TC) gastroenteritis gut microbiome non-targeted metabolism <i>Salmonella</i> |
| title | Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics |
| title_full | Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics |
| title_fullStr | Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics |
| title_full_unstemmed | Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics |
| title_short | Treatment-Related Mechanisms of Tibetan Medicine <i>Terminalia chebula</i> (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin <i>Salmonella</i> Determined Using Intestinal Microbiome Analysis and Metabolomics |
| title_sort | treatment related mechanisms of tibetan medicine i terminalia chebula i tc aqueous extract against mouse gastroenteritis caused by yak origin i salmonella i determined using intestinal microbiome analysis and metabolomics |
| topic | <i>Terminalia chebula</i> (TC) gastroenteritis gut microbiome non-targeted metabolism <i>Salmonella</i> |
| url | https://www.mdpi.com/2076-2615/15/5/755 |
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