Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis

Xuetongsu (XTS, Schisanlactone E) is one of the main active compounds and considered as the star molecule isolated from Kadsura heteroclita (Roxb.) Craib. In order to improve XTS anti-tumour bioactivities, a series of novel XTS derivatives were designed and synthesised by introducing an amide bond a...

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Main Authors: Qi Jiang, Hui Zhong, Cong Wu, Jia Li, Jingmin Chen, Xudong Zhou, Bin Li, Huanghe Yu, Wei Wang, Wenbing Sheng
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
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Online Access:https://www.tandfonline.com/doi/10.1080/14756366.2025.2482140
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author Qi Jiang
Hui Zhong
Cong Wu
Jia Li
Jingmin Chen
Xudong Zhou
Bin Li
Huanghe Yu
Wei Wang
Wenbing Sheng
author_facet Qi Jiang
Hui Zhong
Cong Wu
Jia Li
Jingmin Chen
Xudong Zhou
Bin Li
Huanghe Yu
Wei Wang
Wenbing Sheng
author_sort Qi Jiang
collection DOAJ
description Xuetongsu (XTS, Schisanlactone E) is one of the main active compounds and considered as the star molecule isolated from Kadsura heteroclita (Roxb.) Craib. In order to improve XTS anti-tumour bioactivities, a series of novel XTS derivatives were designed and synthesised by introducing an amide bond at the parent. Anti-proliferative assays on four different human tumour cell lines (BGC-823, HepG-2, HCT-116, and MCF-7) showed that the anti-tumour activities of most derivatives increased greatly compared to the parent XTS, and especially, compounds A-7, A-14, and A-18 exhibited multiple anti-tumour effects. Among them, compound A-7 has the best biological activities on the four tumour cell lines with the IC50 values ranging from 13.86 to 20.71 μM, which could significantly increase the fraction of apoptotic cells according to flow cytometry experience. Further study demonstrated that A-7 could induce apoptosis on HepG-2 cells through influencing the key apoptotic related proteins, such as Bcl-2, Bax, and cleaved Caspase-3.
format Article
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institution OA Journals
issn 1475-6366
1475-6374
language English
publishDate 2025-12-01
publisher Taylor & Francis Group
record_format Article
series Journal of Enzyme Inhibition and Medicinal Chemistry
spelling doaj-art-79728f3e436149dfb4f3bfa9303d25172025-08-20T02:16:21ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742025-12-0140110.1080/14756366.2025.2482140Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosisQi Jiang0Hui Zhong1Cong Wu2Jia Li3Jingmin Chen4Xudong Zhou5Bin Li6Huanghe Yu7Wei Wang8Wenbing Sheng9School of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaXuetongsu (XTS, Schisanlactone E) is one of the main active compounds and considered as the star molecule isolated from Kadsura heteroclita (Roxb.) Craib. In order to improve XTS anti-tumour bioactivities, a series of novel XTS derivatives were designed and synthesised by introducing an amide bond at the parent. Anti-proliferative assays on four different human tumour cell lines (BGC-823, HepG-2, HCT-116, and MCF-7) showed that the anti-tumour activities of most derivatives increased greatly compared to the parent XTS, and especially, compounds A-7, A-14, and A-18 exhibited multiple anti-tumour effects. Among them, compound A-7 has the best biological activities on the four tumour cell lines with the IC50 values ranging from 13.86 to 20.71 μM, which could significantly increase the fraction of apoptotic cells according to flow cytometry experience. Further study demonstrated that A-7 could induce apoptosis on HepG-2 cells through influencing the key apoptotic related proteins, such as Bcl-2, Bax, and cleaved Caspase-3.https://www.tandfonline.com/doi/10.1080/14756366.2025.2482140Xuetongsuanti-tumor bioactivityapoptosisapoptotic related proteinsmolecular docking
spellingShingle Qi Jiang
Hui Zhong
Cong Wu
Jia Li
Jingmin Chen
Xudong Zhou
Bin Li
Huanghe Yu
Wei Wang
Wenbing Sheng
Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
Journal of Enzyme Inhibition and Medicinal Chemistry
Xuetongsu
anti-tumor bioactivity
apoptosis
apoptotic related proteins
molecular docking
title Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
title_full Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
title_fullStr Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
title_full_unstemmed Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
title_short Design, synthesis and biological activity of novel Xuetongsu derivatives as potential anticancer agents by inducing apoptosis
title_sort design synthesis and biological activity of novel xuetongsu derivatives as potential anticancer agents by inducing apoptosis
topic Xuetongsu
anti-tumor bioactivity
apoptosis
apoptotic related proteins
molecular docking
url https://www.tandfonline.com/doi/10.1080/14756366.2025.2482140
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