Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5594370 |
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| author | Lifang Zhang Xinping Li Congying Shi Gaoying Ran Yuting Peng Shuguang Zeng Yan He |
| author_facet | Lifang Zhang Xinping Li Congying Shi Gaoying Ran Yuting Peng Shuguang Zeng Yan He |
| author_sort | Lifang Zhang |
| collection | DOAJ |
| description | Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs. |
| format | Article |
| id | doaj-art-796f269424b04fb08df807d6355d34d3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-796f269424b04fb08df807d6355d34d32025-02-03T06:46:02ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/55943705594370Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCsLifang Zhang0Xinping Li1Congying Shi2Gaoying Ran3Yuting Peng4Shuguang Zeng5Yan He6Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaClinical Application Center, Guangdong Cord Bank, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaSkeletal Biology Research Center, Department of Oral Maxillofacial Surgery, Harvard School of Dental Medicine, Boston, 02114 MA, USAAngiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.http://dx.doi.org/10.1155/2021/5594370 |
| spellingShingle | Lifang Zhang Xinping Li Congying Shi Gaoying Ran Yuting Peng Shuguang Zeng Yan He Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs Stem Cells International |
| title | Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs |
| title_full | Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs |
| title_fullStr | Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs |
| title_full_unstemmed | Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs |
| title_short | Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs |
| title_sort | biocompatibility and angiogenic effect of chitosan graphene oxide hydrogel scaffolds on epcs |
| url | http://dx.doi.org/10.1155/2021/5594370 |
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