Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs

Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by...

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Main Authors: Lifang Zhang, Xinping Li, Congying Shi, Gaoying Ran, Yuting Peng, Shuguang Zeng, Yan He
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2021/5594370
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author Lifang Zhang
Xinping Li
Congying Shi
Gaoying Ran
Yuting Peng
Shuguang Zeng
Yan He
author_facet Lifang Zhang
Xinping Li
Congying Shi
Gaoying Ran
Yuting Peng
Shuguang Zeng
Yan He
author_sort Lifang Zhang
collection DOAJ
description Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.
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institution Kabale University
issn 1687-966X
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language English
publishDate 2021-01-01
publisher Wiley
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series Stem Cells International
spelling doaj-art-796f269424b04fb08df807d6355d34d32025-02-03T06:46:02ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/55943705594370Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCsLifang Zhang0Xinping Li1Congying Shi2Gaoying Ran3Yuting Peng4Shuguang Zeng5Yan He6Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaClinical Application Center, Guangdong Cord Bank, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaDepartment of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, 510000 Guangzhou, Guangdong, ChinaSkeletal Biology Research Center, Department of Oral Maxillofacial Surgery, Harvard School of Dental Medicine, Boston, 02114 MA, USAAngiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.http://dx.doi.org/10.1155/2021/5594370
spellingShingle Lifang Zhang
Xinping Li
Congying Shi
Gaoying Ran
Yuting Peng
Shuguang Zeng
Yan He
Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
Stem Cells International
title Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_full Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_fullStr Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_full_unstemmed Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_short Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_sort biocompatibility and angiogenic effect of chitosan graphene oxide hydrogel scaffolds on epcs
url http://dx.doi.org/10.1155/2021/5594370
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