Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking
PCR-based genome walking is one of the prevalent techniques implemented to acquire unknown flanking genomic DNAs. The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. Therefore, this technique has advance...
Saved in:
| Main Authors: | Hongjing Wu, Hao Pan, Haixing Li |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-01-01
|
| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5161&type=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences
by: Yao-Guang Liu, et al.
Published: (2007-11-01) -
Directional Genome Walking Using PCR
by: R.N. Mishra, et al.
Published: (2002-10-01) -
Computation-assisted SiteFinding- PCR for isolating flanking sequence tags in rice
by: Hongru Wang, et al.
Published: (2011-12-01) -
Modified Inverse PCR Method for Cloning the Flanking Sequences from Human Cell Pools
by: Zhu-Hong Li, et al.
Published: (1999-10-01) -
Use of data-biased random walks on graphs for the retrieval of context-specific networks from genomic data.
by: Kakajan Komurov, et al.
Published: (2010-08-01)