Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment
Purpose. The antitumor activity of a novel alginate (ALG) polymer-based particle that contained paclitaxel (PTX) was evaluated using human primary breast cancer cells. Materials and Methods. PTX was combined with ALG in a nanoparticle as a drug delivery system designed to improve breast cancer tumor...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2016/7549372 |
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| author | Ahmed A. Markeb Nagwa A. El-Maali Douaa M. Sayed Amany Osama Mohamed A. Y. Abdel-Malek Amen H. Zaki Mostafa E. A. Elwanis James J. Driscoll |
| author_facet | Ahmed A. Markeb Nagwa A. El-Maali Douaa M. Sayed Amany Osama Mohamed A. Y. Abdel-Malek Amen H. Zaki Mostafa E. A. Elwanis James J. Driscoll |
| author_sort | Ahmed A. Markeb |
| collection | DOAJ |
| description | Purpose. The antitumor activity of a novel alginate (ALG) polymer-based particle that contained paclitaxel (PTX) was evaluated using human primary breast cancer cells. Materials and Methods. PTX was combined with ALG in a nanoparticle as a drug delivery system designed to improve breast cancer tumor cell killing. PTX-ALG nanoparticles were first synthesized by nanoemulsification polymer cross-linking methods that improved the aqueous solubility. Structural and biophysical properties of the PTX-ALG nanoparticles were then determined by transmission electron microscopy (TEM) and high performance liquid chromatography (HPLC) fluorescence. The effect on cell cycle progression and apoptosis was determined using flow cytometry. Results. PTX-ALG nanoparticles were prepared and characterized by ultraviolet (UV)/visible (VIS), HPLC fluorescence, and TEM. PTX-ALG nanoparticles demonstrated increased hydrophobicity and solubility over PTX alone. Synthetically engineered PTX-ALG nanoparticles promoted cell-cycle arrest, reduced viability, and induced apoptosis in human primary patient breast cancer cells superior to those of PTX alone. Conclusion. Taken together, our results demonstrate that PTX-ALG nanoparticles represent an innovative, nanoscale delivery system for the administration of anticancer agents that may avoid the adverse toxicities with enhanced antitumor effects to improve the treatment of breast cancer patients. |
| format | Article |
| id | doaj-art-795f87ac325345829273f740b34ab865 |
| institution | Kabale University |
| issn | 2090-3170 2090-3189 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-795f87ac325345829273f740b34ab8652025-02-03T01:24:55ZengWileyInternational Journal of Breast Cancer2090-31702090-31892016-01-01201610.1155/2016/75493727549372Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer TreatmentAhmed A. Markeb0Nagwa A. El-Maali1Douaa M. Sayed2Amany Osama3Mohamed A. Y. Abdel-Malek4Amen H. Zaki5Mostafa E. A. Elwanis6James J. Driscoll7Department of Chemistry, Faculty of Science, Assiut University, Assiut 71516, EgyptDepartment of Chemistry, Faculty of Science, Assiut University, Assiut 71516, EgyptDepartment of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut 71516, EgyptDepartment of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptDepartment of Medical Oncology, South Egypt Cancer Institute, Assiut University, Assiut, EgyptDepartment of Radiotherapy, South Egypt Cancer Institute, Assiut University, Assiut, EgyptThe Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, Cincinnati, OH, USAPurpose. The antitumor activity of a novel alginate (ALG) polymer-based particle that contained paclitaxel (PTX) was evaluated using human primary breast cancer cells. Materials and Methods. PTX was combined with ALG in a nanoparticle as a drug delivery system designed to improve breast cancer tumor cell killing. PTX-ALG nanoparticles were first synthesized by nanoemulsification polymer cross-linking methods that improved the aqueous solubility. Structural and biophysical properties of the PTX-ALG nanoparticles were then determined by transmission electron microscopy (TEM) and high performance liquid chromatography (HPLC) fluorescence. The effect on cell cycle progression and apoptosis was determined using flow cytometry. Results. PTX-ALG nanoparticles were prepared and characterized by ultraviolet (UV)/visible (VIS), HPLC fluorescence, and TEM. PTX-ALG nanoparticles demonstrated increased hydrophobicity and solubility over PTX alone. Synthetically engineered PTX-ALG nanoparticles promoted cell-cycle arrest, reduced viability, and induced apoptosis in human primary patient breast cancer cells superior to those of PTX alone. Conclusion. Taken together, our results demonstrate that PTX-ALG nanoparticles represent an innovative, nanoscale delivery system for the administration of anticancer agents that may avoid the adverse toxicities with enhanced antitumor effects to improve the treatment of breast cancer patients.http://dx.doi.org/10.1155/2016/7549372 |
| spellingShingle | Ahmed A. Markeb Nagwa A. El-Maali Douaa M. Sayed Amany Osama Mohamed A. Y. Abdel-Malek Amen H. Zaki Mostafa E. A. Elwanis James J. Driscoll Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment International Journal of Breast Cancer |
| title | Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment |
| title_full | Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment |
| title_fullStr | Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment |
| title_full_unstemmed | Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment |
| title_short | Synthesis, Structural Characterization, and Preclinical Efficacy of a Novel Paclitaxel-Loaded Alginate Nanoparticle for Breast Cancer Treatment |
| title_sort | synthesis structural characterization and preclinical efficacy of a novel paclitaxel loaded alginate nanoparticle for breast cancer treatment |
| url | http://dx.doi.org/10.1155/2016/7549372 |
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