Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques
The contact of lasers with biomaterials like tissues, blood, and skin is a central field of research. Invitro irradiation of human blood with laser beams has been studied for decades, to understand the biostimulatory effects on the different blood cells. Laser tissue associations are determined by e...
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Erbil Polytechnic University
2023-09-01
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| Series: | Polytechnic Journal |
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| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/11 |
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| author | Hero O. Hamad Venos S. Abdullah Fouad H. Kamel Nawroz I. Hassan |
| author_facet | Hero O. Hamad Venos S. Abdullah Fouad H. Kamel Nawroz I. Hassan |
| author_sort | Hero O. Hamad |
| collection | DOAJ |
| description | The contact of lasers with biomaterials like tissues, blood, and skin is a central field of research.
Invitro irradiation of human blood with laser beams has been studied for decades, to understand the
biostimulatory effects on the different blood cells. Laser tissue associations are determined by
employing spectroscopic and cutting-edge microscopic technicality. In this study, blood was extracted
under standard laboratory settings from healthy human subjects. The samples were exposed to He -
Ne laser (wavelength ʎ= 650nm, power p= 100mW) and green laser (wavelength ʎ= 530nm, power
p= 500mW).Complete blood count (CBC) and FTIR spectra for non-irradiated samples were compared
to CBC and FTIR spectra of irradiated samples.During exposure to green laser light MCH and PCT
were not affected while under He-Ne laser exposure all CBC parameters were affected. FTIR spectrum
of normal blood laser irradiation indicated that O-H, C=O, C-O, NH, N=O and C-H.As the duration of
exposure to He-Ne laser irradiation on whole blood is decreased for 10, 20, and 30 minutes, the
absorption for all groups, except NH and CO absent peaks for 10 and 20 minutes, yet it is present in
30 minutes.When blood is exposed to green laser, all of the functional groups OH,C=O,CH and N=O
are present, however for control 2 only peaks of CO after irradiation for 30 minutes are absent. The
findings prove that green laser light therapy has a lesser effect on the blood component than He-Ne
lasers. |
| format | Article |
| id | doaj-art-795d5d8a76e14cf186c858dee8fdbacd |
| institution | Kabale University |
| issn | 2707-7799 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Erbil Polytechnic University |
| record_format | Article |
| series | Polytechnic Journal |
| spelling | doaj-art-795d5d8a76e14cf186c858dee8fdbacd2025-08-20T03:35:23ZengErbil Polytechnic UniversityPolytechnic Journal2707-77992023-09-011218997https://doi.org/10.25156/ptj.v12n1y2022.pp89-97Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR TechniquesHero O. Hamad0Venos S. Abdullah1Fouad H. Kamel2Nawroz I. Hassan3Department of Medical Laboratory Technology, College of Erbil Health and Medical Technical, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.Department of Physiotherapy, College of Erbil Health and Medical Technical, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq. Department of Medical Laboratory Technology, College of Erbil Health and Medical Technical, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.Department of Physiotherapy, College of Erbil Health and Medical Technical, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq. The contact of lasers with biomaterials like tissues, blood, and skin is a central field of research. Invitro irradiation of human blood with laser beams has been studied for decades, to understand the biostimulatory effects on the different blood cells. Laser tissue associations are determined by employing spectroscopic and cutting-edge microscopic technicality. In this study, blood was extracted under standard laboratory settings from healthy human subjects. The samples were exposed to He - Ne laser (wavelength ʎ= 650nm, power p= 100mW) and green laser (wavelength ʎ= 530nm, power p= 500mW).Complete blood count (CBC) and FTIR spectra for non-irradiated samples were compared to CBC and FTIR spectra of irradiated samples.During exposure to green laser light MCH and PCT were not affected while under He-Ne laser exposure all CBC parameters were affected. FTIR spectrum of normal blood laser irradiation indicated that O-H, C=O, C-O, NH, N=O and C-H.As the duration of exposure to He-Ne laser irradiation on whole blood is decreased for 10, 20, and 30 minutes, the absorption for all groups, except NH and CO absent peaks for 10 and 20 minutes, yet it is present in 30 minutes.When blood is exposed to green laser, all of the functional groups OH,C=O,CH and N=O are present, however for control 2 only peaks of CO after irradiation for 30 minutes are absent. The findings prove that green laser light therapy has a lesser effect on the blood component than He-Ne lasers.https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/11helium neon (he-ne) laser,green laser,ftir,healthy human blood samples,negativecontrol |
| spellingShingle | Hero O. Hamad Venos S. Abdullah Fouad H. Kamel Nawroz I. Hassan Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques Polytechnic Journal helium neon (he-ne) laser, green laser, ftir, healthy human blood samples, negative control |
| title | Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques |
| title_full | Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques |
| title_fullStr | Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques |
| title_full_unstemmed | Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques |
| title_short | Comparative Study of He – Ne and Green Lasers Effect on Normal Human Blood Invitro Using FTIR Techniques |
| title_sort | comparative study of he ne and green lasers effect on normal human blood invitro using ftir techniques |
| topic | helium neon (he-ne) laser, green laser, ftir, healthy human blood samples, negative control |
| url | https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/11 |
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