Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets
In the present study an isocratic reversed-phase high-performance liquid chromatography method was developed for the estimation of zolpidem in bulk drug and pharmaceutical dosage forms. The quantification was carried out on C18 columns. A mixture of acetonitrile-ammonium acetate (pH=8.0, 0.02 M) (60...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/357890 |
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| author | E. Konoz A. H. Mohsen Sarrafi R. Abdolahnejad M. Bahrami-Zonoz |
| author_facet | E. Konoz A. H. Mohsen Sarrafi R. Abdolahnejad M. Bahrami-Zonoz |
| author_sort | E. Konoz |
| collection | DOAJ |
| description | In the present study an isocratic reversed-phase high-performance liquid chromatography method was developed for the estimation of zolpidem in bulk drug and pharmaceutical dosage forms. The quantification was carried out on C18 columns. A mixture of acetonitrile-ammonium acetate (pH=8.0, 0.02 M) (60 : 40 v/v) was used as the mobile phase, at flow rate of 1.0 mL/min and the determination wavelength at 245 nm. The retention time of zolpidem was found to be 3–5 min. The validation of the proposed method was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness. The linear dynamic range was from 2.5 to 30 μg mL−1. Regression equation was found to be y=0.1416x+0.0183 with correlation coefficient r=0.9996. The percentage recovery obtained for zolpidem was greater than 96.5%. Limit of quantification and limit of detection were found to be 2.5 μg mL−1 and 0.83 μg mL−1, respectively. The developed method can be used for routine quality control analysis of zolpidem in tablet formulations. |
| format | Article |
| id | doaj-art-7946958a1c2f4fc983c97dc4bf758c70 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-7946958a1c2f4fc983c97dc4bf758c702025-02-03T01:04:58ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/357890357890Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and TabletsE. Konoz0A. H. Mohsen Sarrafi1R. Abdolahnejad2M. Bahrami-Zonoz3Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran 13185-768, IranDepartment of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran 13185-768, IranLoghman Pharmaceutical Co., P.O. Box 14155, Tehran 1443, IranDepartment of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran 13185-768, IranIn the present study an isocratic reversed-phase high-performance liquid chromatography method was developed for the estimation of zolpidem in bulk drug and pharmaceutical dosage forms. The quantification was carried out on C18 columns. A mixture of acetonitrile-ammonium acetate (pH=8.0, 0.02 M) (60 : 40 v/v) was used as the mobile phase, at flow rate of 1.0 mL/min and the determination wavelength at 245 nm. The retention time of zolpidem was found to be 3–5 min. The validation of the proposed method was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness. The linear dynamic range was from 2.5 to 30 μg mL−1. Regression equation was found to be y=0.1416x+0.0183 with correlation coefficient r=0.9996. The percentage recovery obtained for zolpidem was greater than 96.5%. Limit of quantification and limit of detection were found to be 2.5 μg mL−1 and 0.83 μg mL−1, respectively. The developed method can be used for routine quality control analysis of zolpidem in tablet formulations.http://dx.doi.org/10.1155/2013/357890 |
| spellingShingle | E. Konoz A. H. Mohsen Sarrafi R. Abdolahnejad M. Bahrami-Zonoz Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets Journal of Chemistry |
| title | Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets |
| title_full | Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets |
| title_fullStr | Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets |
| title_full_unstemmed | Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets |
| title_short | Development and Validation of a Reversed-Phase HPLC Method for the Estimation of Zolpidem in Bulk Drug and Tablets |
| title_sort | development and validation of a reversed phase hplc method for the estimation of zolpidem in bulk drug and tablets |
| url | http://dx.doi.org/10.1155/2013/357890 |
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