Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing
Radix polygalae, the dried roots of Polygala tenuifolia and P. sibirica, is one of the most well-known traditional Chinese medicinal plants. Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties. To...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2015/782635 |
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| author | Hongling Tian Xiaoshuang Xu Fusheng Zhang Yaoqin Wang Shuhong Guo Xuemei Qin Guanhua Du |
| author_facet | Hongling Tian Xiaoshuang Xu Fusheng Zhang Yaoqin Wang Shuhong Guo Xuemei Qin Guanhua Du |
| author_sort | Hongling Tian |
| collection | DOAJ |
| description | Radix polygalae, the dried roots of Polygala tenuifolia and P. sibirica, is one of the most well-known traditional Chinese medicinal plants. Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties. To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P. tenuifolia, we applied Illumina sequencing technology and de novo assembly. We also applied this technique to gain an overview of P. tenuifolia transcriptome from samples with different years. Using Illumina sequencing, approximately 67.2% of unique sequences were annotated by basic local alignment search tool similarity searches against public sequence databases. We classified the annotated unigenes by using Nr, Nt, GO, COG, and KEGG databases compared with NCBI. We also obtained many candidates CYP450s and UGTs by the analysis of genes in the secondary metabolite biosynthetic pathways, including putative terpenoid backbone and phenylpropanoid biosynthesis pathway. With this transcriptome sequencing, future genetic and genomics studies related to the molecular mechanisms associated with the chemical composition of P. tenuifolia may be improved. Genes involved in the enrichment of secondary metabolite biosynthesis-related pathways could enhance the potential applications of P. tenuifolia in pharmaceutical industries. |
| format | Article |
| id | doaj-art-7939c2888af54787adf9117aaa884d2f |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-7939c2888af54787adf9117aaa884d2f2025-02-03T01:30:50ZengWileyInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/782635782635Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina SequencingHongling Tian0Xiaoshuang Xu1Fusheng Zhang2Yaoqin Wang3Shuhong Guo4Xuemei Qin5Guanhua Du6Research Institute of Economics Crop, Shanxi Academy of Agriculture Science, Fenyang, Shanxi 032200, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, No. 92 Wucheng Road, Taiyuan, Shanxi 030006, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, No. 92 Wucheng Road, Taiyuan, Shanxi 030006, ChinaResearch Institute of Economics Crop, Shanxi Academy of Agriculture Science, Fenyang, Shanxi 032200, ChinaResearch Institute of Economics Crop, Shanxi Academy of Agriculture Science, Fenyang, Shanxi 032200, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, No. 92 Wucheng Road, Taiyuan, Shanxi 030006, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, No. 92 Wucheng Road, Taiyuan, Shanxi 030006, ChinaRadix polygalae, the dried roots of Polygala tenuifolia and P. sibirica, is one of the most well-known traditional Chinese medicinal plants. Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties. To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P. tenuifolia, we applied Illumina sequencing technology and de novo assembly. We also applied this technique to gain an overview of P. tenuifolia transcriptome from samples with different years. Using Illumina sequencing, approximately 67.2% of unique sequences were annotated by basic local alignment search tool similarity searches against public sequence databases. We classified the annotated unigenes by using Nr, Nt, GO, COG, and KEGG databases compared with NCBI. We also obtained many candidates CYP450s and UGTs by the analysis of genes in the secondary metabolite biosynthetic pathways, including putative terpenoid backbone and phenylpropanoid biosynthesis pathway. With this transcriptome sequencing, future genetic and genomics studies related to the molecular mechanisms associated with the chemical composition of P. tenuifolia may be improved. Genes involved in the enrichment of secondary metabolite biosynthesis-related pathways could enhance the potential applications of P. tenuifolia in pharmaceutical industries.http://dx.doi.org/10.1155/2015/782635 |
| spellingShingle | Hongling Tian Xiaoshuang Xu Fusheng Zhang Yaoqin Wang Shuhong Guo Xuemei Qin Guanhua Du Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing International Journal of Genomics |
| title | Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing |
| title_full | Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing |
| title_fullStr | Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing |
| title_full_unstemmed | Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing |
| title_short | Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing |
| title_sort | analysis of polygala tenuifolia transcriptome and description of secondary metabolite biosynthetic pathways by illumina sequencing |
| url | http://dx.doi.org/10.1155/2015/782635 |
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