The new technologies of high-throughput single-cell RNA sequencing
A wealth of genome and transcriptome data obtained using new generation sequencing (NGS) technologies for whole organisms could not answer many questions in oncology, immunology, physiology, neurobiology, zoology and other fields of science and medicine. Since the cell is the basis for the living of...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2200 |
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| author | E. A. Vodiasova E. S. Chelebieva O. N. Kuleshova |
| author_facet | E. A. Vodiasova E. S. Chelebieva O. N. Kuleshova |
| author_sort | E. A. Vodiasova |
| collection | DOAJ |
| description | A wealth of genome and transcriptome data obtained using new generation sequencing (NGS) technologies for whole organisms could not answer many questions in oncology, immunology, physiology, neurobiology, zoology and other fields of science and medicine. Since the cell is the basis for the living of all unicellular and multicellular organisms, it is necessary to study the biological processes at its level. This understanding gave impetus to the development of a new direction – the creation of technologies that allow working with individual cells (single-cell technology). The rapid development of not only instruments, but also various advanced protocols for working with single cells is due to the relevance of these studies in many fields of science and medicine. Studying the features of various stages of ontogenesis, identifying patterns of cell differentiation and subsequent tissue development, conducting genomic and transcriptome analyses in various areas of medicine (especially in demand in immunology and oncology), identifying cell types and states, patterns of biochemical and physiological processes using single cell technologies, allows the comprehensive research to be conducted at a new level. The first RNA-sequencing technologies of individual cell transcriptomes (scRNA-seq) captured no more than one hundred cells at a time, which was insufficient due to the detection of high cell heterogeneity, existence of the minor cell types (which were not detected by morphology) and complex regulatory pathways. The unique techniques for isolating, capturing and sequencing transcripts of tens of thousands of cells at a time are evolving now. However, new technologies have certain differences both at the sample preparation stage and during the bioinformatics analysis. In the paper we consider the most effective methods of multiple parallel scRNA-seq using the example of 10XGenomics, as well as the specifics of such an experiment, further bioinformatics analysis of the data, future outlook and applications of new high-performance technologies. |
| format | Article |
| id | doaj-art-792a558961f247528a8ddfcc7c08ff73 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-792a558961f247528a8ddfcc7c08ff732025-02-01T09:58:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-08-0123550851810.18699/VJ19.520938The new technologies of high-throughput single-cell RNA sequencingE. A. Vodiasova0E. S. Chelebieva1O. N. Kuleshova2A.O. Kovalevsky Institute of Biology of the Southern Seas, RASA.O. Kovalevsky Institute of Biology of the Southern Seas, RASA.O. Kovalevsky Institute of Biology of the Southern Seas, RASA wealth of genome and transcriptome data obtained using new generation sequencing (NGS) technologies for whole organisms could not answer many questions in oncology, immunology, physiology, neurobiology, zoology and other fields of science and medicine. Since the cell is the basis for the living of all unicellular and multicellular organisms, it is necessary to study the biological processes at its level. This understanding gave impetus to the development of a new direction – the creation of technologies that allow working with individual cells (single-cell technology). The rapid development of not only instruments, but also various advanced protocols for working with single cells is due to the relevance of these studies in many fields of science and medicine. Studying the features of various stages of ontogenesis, identifying patterns of cell differentiation and subsequent tissue development, conducting genomic and transcriptome analyses in various areas of medicine (especially in demand in immunology and oncology), identifying cell types and states, patterns of biochemical and physiological processes using single cell technologies, allows the comprehensive research to be conducted at a new level. The first RNA-sequencing technologies of individual cell transcriptomes (scRNA-seq) captured no more than one hundred cells at a time, which was insufficient due to the detection of high cell heterogeneity, existence of the minor cell types (which were not detected by morphology) and complex regulatory pathways. The unique techniques for isolating, capturing and sequencing transcripts of tens of thousands of cells at a time are evolving now. However, new technologies have certain differences both at the sample preparation stage and during the bioinformatics analysis. In the paper we consider the most effective methods of multiple parallel scRNA-seq using the example of 10XGenomics, as well as the specifics of such an experiment, further bioinformatics analysis of the data, future outlook and applications of new high-performance technologies.https://vavilov.elpub.ru/jour/article/view/2200scrna-seqtranscriptomicschromium 10xgenomicssequencingsingle cell |
| spellingShingle | E. A. Vodiasova E. S. Chelebieva O. N. Kuleshova The new technologies of high-throughput single-cell RNA sequencing Вавиловский журнал генетики и селекции scrna-seq transcriptomics chromium 10xgenomics sequencing single cell |
| title | The new technologies of high-throughput single-cell RNA sequencing |
| title_full | The new technologies of high-throughput single-cell RNA sequencing |
| title_fullStr | The new technologies of high-throughput single-cell RNA sequencing |
| title_full_unstemmed | The new technologies of high-throughput single-cell RNA sequencing |
| title_short | The new technologies of high-throughput single-cell RNA sequencing |
| title_sort | new technologies of high throughput single cell rna sequencing |
| topic | scrna-seq transcriptomics chromium 10xgenomics sequencing single cell |
| url | https://vavilov.elpub.ru/jour/article/view/2200 |
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